RB32A_DICDI
ID RB32A_DICDI Reviewed; 219 AA.
AC Q54QR3; Q9XYN6;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Ras-related protein Rab-32A;
GN Name=rab32A; Synonyms=rabE; ORFNames=DDB_G0283603;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RX PubMed=10025509; DOI=10.1089/104454999315628;
RA Norian L., Dragoi I.A., O'Halloran T.;
RT "Molecular characterization of rabE, a developmentally regulated
RT Dictyostelium homolog of mammalian rab GTPases.";
RL DNA Cell Biol. 18:59-64(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=AX2;
RX PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA Soldati T.;
RT "Proteomics fingerprinting of phagosome maturation and evidence for the
RT role of a Galpha during uptake.";
RL Mol. Cell. Proteomics 5:2228-2243(2006).
CC -!- DEVELOPMENTAL STAGE: Expression under developmental regulation, the
CC mRNA starts to be expressed after 8 hours of development.
CC {ECO:0000269|PubMed:10025509}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
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DR EMBL; AF116859; AAD23450.1; -; Genomic_DNA.
DR EMBL; AAFI02000056; EAL65568.1; -; Genomic_DNA.
DR RefSeq; XP_638960.1; XM_633868.1.
DR AlphaFoldDB; Q54QR3; -.
DR SMR; Q54QR3; -.
DR STRING; 44689.DDB0201639; -.
DR PaxDb; Q54QR3; -.
DR EnsemblProtists; EAL65568; EAL65568; DDB_G0283603.
DR GeneID; 8624206; -.
DR KEGG; ddi:DDB_G0283603; -.
DR dictyBase; DDB_G0283603; rab32A.
DR eggNOG; KOG4423; Eukaryota.
DR HOGENOM; CLU_041217_10_6_1; -.
DR InParanoid; Q54QR3; -.
DR OMA; MHYKSTI; -.
DR PhylomeDB; Q54QR3; -.
DR Reactome; R-DDI-8873719; RAB geranylgeranylation.
DR Reactome; R-DDI-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR PRO; PR:Q54QR3; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005811; C:lipid droplet; HDA:dictyBase.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0140220; C:pathogen-containing vacuole; HDA:dictyBase.
DR GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR GO; GO:0005802; C:trans-Golgi network; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0032438; P:melanosome organization; IBA:GO_Central.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:InterPro.
DR CDD; cd04107; Rab32_Rab38; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR030697; Rab29/Rab38/Rab32.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 1: Evidence at protein level;
KW GTP-binding; Lipoprotein; Nucleotide-binding; Prenylation;
KW Reference proteome.
FT CHAIN 1..219
FT /note="Ras-related protein Rab-32A"
FT /id="PRO_0000330641"
FT REGION 192..219
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 44..52
FT /note="Effector region"
FT /evidence="ECO:0000250"
FT COMPBIAS 198..219
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 22..29
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 71..75
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 134..137
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT LIPID 217
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
FT LIPID 218
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
FT CONFLICT 1..8
FT /note="MSNNPADD -> MTNKNKLLNYLCGGADA (in Ref. 1; AAD23450)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 219 AA; 24705 MW; 71C862E6012E5A0C CRC64;
MSNNPADDEG YNEYLYKILV VGDIGTGKTS IIKRFVHNIF SMHYKSTIGV DFALKVINWD
PKTEVRLQLW DIAGQERFGS MTRVYYKEAV GAMITFDVTR MSTFEAVAKW KADIDSKVTY
GADEKPIPVV LLANKCDLGK DAFIKTANDM DKYCKDNGFI GWFETSAKEN MNIEKAARFL
VDHILKNDVR RNQPIEGTIQ PGDLNKQPQP TSTGPSCCK
