ID   RB33B_PONAB             Reviewed;         229 AA.
AC   Q5R615;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Ras-related protein Rab-33B;
GN   Name=RAB33B;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain cortex;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Protein transport. Acts, in coordination with RAB6A, to
CC       regulate intra-Golgi retrograde trafficking (By similarity). It is
CC       involved in autophagy, acting as a modulator of autophagosome formation
CC       (By similarity). {ECO:0000250}.
CC   -!- ACTIVITY REGULATION: Regulated by a guanine nucleotide-exchange factor
CC       (GEF) and a GTPase-activating protein (GAP) and alternates between an
CC       inactive GDP-bound and an active GTP-bound form. In vitro, SGSM2 acts
CC       as its GAP and inactivates it by stimulating its GTPase activity.
CC       {ECO:0000250|UniProtKB:Q9H082}.
CC   -!- SUBUNIT: Interacts with ATG16L1; the interaction is important for
CC       autophagosome formation. Interacts with ATG16L2; however interaction is
CC       approximately hundred times lower than for ATG16L1 (By similarity).
CC       Interacts with RIC1 (via C-terminus domain); the interaction is direct
CC       with a preference for RAB33B-GTP. Interacts with RGP1.
CC       {ECO:0000250|UniProtKB:O35963, ECO:0000250|UniProtKB:Q9H082}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC       {ECO:0000250|UniProtKB:O35963}; Lipid-anchor {ECO:0000305}. Golgi
CC       apparatus, cis-Golgi network {ECO:0000250|UniProtKB:O35963}. Note=Under
CC       starvation conditions punctate RAB33B-positive structures are often
CC       observed in the cytoplasm. {ECO:0000250|UniProtKB:O35963}.
CC   -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC       {ECO:0000305}.
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DR   EMBL; CR860685; CAH92801.1; -; mRNA.
DR   RefSeq; NP_001126634.1; NM_001133162.1.
DR   AlphaFoldDB; Q5R615; -.
DR   SMR; Q5R615; -.
DR   STRING; 9601.ENSPPYP00000016838; -.
DR   PRIDE; Q5R615; -.
DR   Ensembl; ENSPPYT00000037899; ENSPPYP00000043708; ENSPPYG00000015072.
DR   GeneID; 100173632; -.
DR   KEGG; pon:100173632; -.
DR   CTD; 83452; -.
DR   eggNOG; KOG0084; Eukaryota.
DR   GeneTree; ENSGT00940000157090; -.
DR   HOGENOM; CLU_041217_10_3_1; -.
DR   InParanoid; Q5R615; -.
DR   OMA; QQFADSH; -.
DR   OrthoDB; 1081271at2759; -.
DR   TreeFam; TF300097; -.
DR   Proteomes; UP000001595; Chromosome 4.
DR   GO; GO:0005796; C:Golgi lumen; ISS:UniProtKB.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:Ensembl.
DR   GO; GO:0000045; P:autophagosome assembly; ISS:UniProtKB.
DR   GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IEA:Ensembl.
DR   GO; GO:1903434; P:negative regulation of constitutive secretory pathway; IEA:Ensembl.
DR   GO; GO:0034067; P:protein localization to Golgi apparatus; IEA:Ensembl.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0032482; P:Rab protein signal transduction; IEA:InterPro.
DR   GO; GO:1903358; P:regulation of Golgi organization; IEA:Ensembl.
DR   GO; GO:2000156; P:regulation of retrograde vesicle-mediated transport, Golgi to ER; ISS:UniProtKB.
DR   GO; GO:0048705; P:skeletal system morphogenesis; IEA:Ensembl.
DR   CDD; cd04115; Rab33B_Rab33A; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR041822; Rab33A/B.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR001806; Small_GTPase.
DR   Pfam; PF00071; Ras; 1.
DR   SMART; SM00174; RHO; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51419; RAB; 1.
PE   2: Evidence at transcript level;
KW   Autophagy; Golgi apparatus; GTP-binding; Lipoprotein; Membrane;
KW   Methylation; Nucleotide-binding; Prenylation; Protein transport;
KW   Reference proteome; Transport.
FT   CHAIN           1..229
FT                   /note="Ras-related protein Rab-33B"
FT                   /id="PRO_0000261125"
FT   BINDING         39..46
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         88..92
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         148..151
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         229
FT                   /note="Cysteine methyl ester"
FT                   /evidence="ECO:0000250"
FT   LIPID           227
FT                   /note="S-geranylgeranyl cysteine"
FT                   /evidence="ECO:0000250"
FT   LIPID           229
FT                   /note="S-geranylgeranyl cysteine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   229 AA;  25690 MW;  FE28AD6CC8170099 CRC64;
     MAEEMESSLE ASFSSSGAVS GASGFLPPAR SRIFKIIVIG DSNVGKTCLT YRFCAGRFPD
     RTEATIGVDF RERAVEIDGE RIKIQLWDTA GQERFRKSMV QHYYRNVHAV VFVYDMTNMA
     SFHSLPSWIE ECKQHLLASD IPRILVGNKC DLRSAIQVPT DLAQKFADTH SMPLFETSAK
     NPNDNDHVEA IFMTLAHKLK SHKPLMLSQP PDNGIILKPE PKPAMTCWC