RB97D_DROME
ID RB97D_DROME Reviewed; 471 AA.
AC Q02926; Q8IHB8; Q9VBA9;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Ribonucleoprotein RB97D;
GN Name=Rb97D; Synonyms=Hrb97D; ORFNames=CG6354;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS A AND B), AND FUNCTION.
RX PubMed=8502565; DOI=10.1093/nar/21.9.2229;
RA Karsch-Mizrachi I., Haynes S.R.;
RT "The Rb97D gene encodes a potential RNA-binding protein required for
RT spermatogenesis in Drosophila.";
RL Nucleic Acids Res. 21:2229-2235(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Testis;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Required for spermatogenesis. Could be required to process a
CC specific transcript essential for spermatogenesis.
CC {ECO:0000269|PubMed:8502565}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B;
CC IsoId=Q02926-1; Sequence=Displayed;
CC Name=A;
CC IsoId=Q02926-2; Sequence=VSP_005808, VSP_005809;
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DR EMBL; L02106; AAA99873.1; -; Genomic_DNA.
DR EMBL; L02106; AAA99872.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF56633.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN14092.1; -; Genomic_DNA.
DR EMBL; BT001320; AAN71075.1; ALT_SEQ; mRNA.
DR PIR; S33679; S33679.
DR PIR; S33680; S33680.
DR RefSeq; NP_001014671.1; NM_001014671.2. [Q02926-1]
DR RefSeq; NP_001014672.1; NM_001014672.2. [Q02926-1]
DR RefSeq; NP_001014673.1; NM_001014673.2. [Q02926-2]
DR RefSeq; NP_001014674.1; NM_001014674.2. [Q02926-1]
DR RefSeq; NP_001014675.1; NM_001014675.2.
DR RefSeq; NP_001014676.1; NM_001014676.2.
DR RefSeq; NP_001014677.1; NM_001014677.2. [Q02926-2]
DR RefSeq; NP_524520.1; NM_079796.5. [Q02926-1]
DR RefSeq; NP_733172.1; NM_170293.3. [Q02926-2]
DR AlphaFoldDB; Q02926; -.
DR SMR; Q02926; -.
DR BioGRID; 68122; 6.
DR DIP; DIP-18137N; -.
DR IntAct; Q02926; 2.
DR STRING; 7227.FBpp0084463; -.
DR PaxDb; Q02926; -.
DR DNASU; 43231; -.
DR EnsemblMetazoa; FBtr0085092; FBpp0084462; FBgn0004903. [Q02926-2]
DR EnsemblMetazoa; FBtr0085093; FBpp0084463; FBgn0004903. [Q02926-1]
DR EnsemblMetazoa; FBtr0100334; FBpp0099739; FBgn0004903. [Q02926-2]
DR EnsemblMetazoa; FBtr0100337; FBpp0099742; FBgn0004903. [Q02926-1]
DR EnsemblMetazoa; FBtr0100338; FBpp0099743; FBgn0004903. [Q02926-2]
DR EnsemblMetazoa; FBtr0100339; FBpp0099744; FBgn0004903. [Q02926-1]
DR EnsemblMetazoa; FBtr0100340; FBpp0099745; FBgn0004903. [Q02926-1]
DR GeneID; 43231; -.
DR KEGG; dme:Dmel_CG6354; -.
DR CTD; 43231; -.
DR FlyBase; FBgn0004903; Rb97D.
DR VEuPathDB; VectorBase:FBgn0004903; -.
DR eggNOG; KOG0118; Eukaryota.
DR GeneTree; ENSGT00940000167175; -.
DR InParanoid; Q02926; -.
DR PhylomeDB; Q02926; -.
DR BioGRID-ORCS; 43231; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Rb97D; fly.
DR GenomeRNAi; 43231; -.
DR PRO; PR:Q02926; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0004903; Expressed in testis and 27 other tissues.
DR ExpressionAtlas; Q02926; baseline and differential.
DR Genevisible; Q02926; DM.
DR GO; GO:0003729; F:mRNA binding; ISS:FlyBase.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; Differentiation;
KW Reference proteome; Repeat; RNA-binding; Spermatogenesis.
FT CHAIN 1..471
FT /note="Ribonucleoprotein RB97D"
FT /id="PRO_0000081751"
FT DOMAIN 32..109
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 123..198
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 221..471
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 221..239
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 240..320
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 331..370
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 371..418
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 457..471
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 422..427
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000305"
FT /id="VSP_005808"
FT VAR_SEQ 428
FT /note="A -> P (in isoform A)"
FT /evidence="ECO:0000305"
FT /id="VSP_005809"
SQ SEQUENCE 471 AA; 51476 MW; 6B044A253A4E7120 CRC64;
MVKDEPLSDE SADVIVLADR EEDDICELEH LRKLFIGGLA PYTTEENLKL FYGQWGKVVD
VVVMRDAATK RSRGFGFITY TKSLMVDRAQ ENRPHIIDGK TVEAKRALPR PERESRETNI
SVKKLFVGGL KDNHDEECLR EYFLQFGNVV SVKLLTDKTT GKRRGFAFVE FDDYDAVDKA
ILKKQHAIKY VHVDVKKSIY NLDKKEKQQP GGLANAIKPS LNQQQQQQGG GQQPPNGNMQ
APSFRPPVPP QQAMGPYQQQ PPPAPMSAPP PNFNYWGPPP PAMPPYYQQP PPQQMNGWGP
YPPPQQNGWN APPPPPPGAQ QWHANQWGCP PPVQQVPPVG AVPPPMGHNG PPPTAPGNWN
MPPPVPGAAP PSHQQQSSQQ PTPQPNFGTG YQQNYGGGPS KHNNMNANRM NPYSAGPPNS
YHTPQPPAYT GYNAGPLPPN GSVPPPTGAK AVGVSNGSVA TGGSANSKYR R
