RBBP5_CAEEL
ID RBBP5_CAEEL Reviewed; 454 AA.
AC Q09309;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 23-APR-2003, sequence version 3.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Retinoblastoma-binding protein homolog 5;
GN Name=rbbp-5; Synonyms=lsy-15 {ECO:0000303|PubMed:21698137};
GN ORFNames=F21H12.1;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=21455483; DOI=10.1371/journal.pgen.1001349;
RA Li T., Kelly W.G.;
RT "A role for Set1/MLL-related components in epigenetic regulation of the
RT Caenorhabditis elegans germ line.";
RL PLoS Genet. 7:E1001349-E1001349(2011).
RN [3] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP 320-TRP--LYS-454.
RX PubMed=21698137; DOI=10.1371/journal.pgen.1002109;
RA Poole R.J., Bashllari E., Cochella L., Flowers E.B., Hobert O.;
RT "A Genome-Wide RNAi Screen for Factors Involved in Neuronal Specification
RT in Caenorhabditis elegans.";
RL PLoS Genet. 7:e1002109-e1002109(2011).
RN [4]
RP IDENTIFICATION IN THE SET2 COMPLEX.
RX PubMed=31602465; DOI=10.1093/nar/gkz880;
RA Beurton F., Stempor P., Caron M., Appert A., Dong Y., Chen R.A., Cluet D.,
RA Coute Y., Herbette M., Huang N., Polveche H., Spichty M., Bedet C.,
RA Ahringer J., Palladino F.;
RT "Physical and functional interaction between SET1/COMPASS complex component
RT CFP-1 and a Sin3S HDAC complex in C. elegans.";
RL Nucleic Acids Res. 47:11164-11180(2019).
CC -!- FUNCTION: Required for di- and trimethylation at 'Lys-4' of histone H3.
CC Regulates left/right asymmetry of ASE sensory neurons, via its role as
CC a component of the SET2 complex (PubMed:21698137).
CC {ECO:0000269|PubMed:21455483, ECO:0000269|PubMed:21698137}.
CC -!- SUBUNIT: Component of the SET2 complex (also known as the SET1/COMPASS
CC complex), which contains at least set-2, swd-2.1, cfp-1, rbbp-5, wdr-
CC 5.1, dpy-30 and ash-2. {ECO:0000269|PubMed:31602465}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21698137}.
CC -!- DEVELOPMENTAL STAGE: Expressed widely during early embryogenesis
CC (PubMed:21698137). Expression is significantly reduced throughout the
CC embryo after formation of the ASE neurons (PubMed:21698137). Expressed
CC in the maternal germline (PubMed:21698137).
CC {ECO:0000269|PubMed:21698137}.
CC -!- DISRUPTION PHENOTYPE: Strongly reduced di- and trimethylation at 'Lys-
CC 4' of histone H3 in embryos and adult germline stem cells. Mutants also
CC display defects in egg laying and fertility with defective germ cell
CC development. RNAi-mediated knockdown disrupts development of the ASE
CC left (ASEL) neuron, causing it to adopt the ASE right (ASER) cell fate
CC (PubMed:21698137). {ECO:0000269|PubMed:21455483,
CC ECO:0000269|PubMed:21698137}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FO080717; CCD66115.1; -; Genomic_DNA.
DR PIR; T16130; T16130.
DR RefSeq; NP_495222.2; NM_062821.8.
DR AlphaFoldDB; Q09309; -.
DR SMR; Q09309; -.
DR BioGRID; 39360; 4.
DR STRING; 6239.F21H12.1; -.
DR iPTMnet; Q09309; -.
DR EPD; Q09309; -.
DR PaxDb; Q09309; -.
DR PeptideAtlas; Q09309; -.
DR EnsemblMetazoa; F21H12.1.1; F21H12.1.1; WBGene00017683.
DR GeneID; 174019; -.
DR KEGG; cel:CELE_F21H12.1; -.
DR UCSC; F21H12.1; c. elegans.
DR CTD; 174019; -.
DR WormBase; F21H12.1; CE32634; WBGene00017683; rbbp-5.
DR eggNOG; KOG1273; Eukaryota.
DR GeneTree; ENSGT00530000064100; -.
DR HOGENOM; CLU_032142_2_2_1; -.
DR InParanoid; Q09309; -.
DR OMA; DYEDDIM; -.
DR OrthoDB; 1001705at2759; -.
DR PhylomeDB; Q09309; -.
DR Reactome; R-CEL-201722; Formation of the beta-catenin:TCF transactivating complex.
DR Reactome; R-CEL-3214841; PKMTs methylate histone lysines.
DR Reactome; R-CEL-8936459; RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function.
DR Reactome; R-CEL-8951664; Neddylation.
DR PRO; PR:Q09309; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00017683; Expressed in embryo and 3 other tissues.
DR GO; GO:0044666; C:MLL3/4 complex; IDA:WormBase.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0048188; C:Set1C/COMPASS complex; IBA:GO_Central.
DR GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR GO; GO:0007281; P:germ cell development; IMP:UniProtKB.
DR GO; GO:0044648; P:histone H3-K4 dimethylation; IMP:UniProtKB.
DR GO; GO:0051568; P:histone H3-K4 methylation; IBA:GO_Central.
DR GO; GO:0080182; P:histone H3-K4 trimethylation; IMP:UniProtKB.
DR GO; GO:0018991; P:oviposition; IMP:UniProtKB.
DR GO; GO:0012501; P:programmed cell death; IMP:UniProtKB.
DR GO; GO:0060290; P:transdifferentiation; IMP:WormBase.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR037850; RBBP5/Swd1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR44040; PTHR44040; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Chromatin regulator; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; WD repeat.
FT CHAIN 1..454
FT /note="Retinoblastoma-binding protein homolog 5"
FT /id="PRO_0000051507"
FT REPEAT 23..64
FT /note="WD 1"
FT REPEAT 65..104
FT /note="WD 2"
FT REPEAT 153..192
FT /note="WD 3"
FT REPEAT 196..235
FT /note="WD 4"
FT REPEAT 248..288
FT /note="WD 5"
FT REPEAT 292..330
FT /note="WD 6"
FT MUTAGEN 320..454
FT /note="Missing: In ot86; defects in left/right asymmetry of
FT ASE sensory neurons."
FT /evidence="ECO:0000269|PubMed:21698137"
SQ SEQUENCE 454 AA; 51336 MW; DB813F64E33433CF CRC64;
MVEILDRTYG AQFPEELECH LDLQNASANC CKFNRWGSIV AVGCTDGRVL IYDFMTRNIA
RTFSAHCLPV SCLSWSRDGR KLLTSSADNS IAMFDVLAGT LLHRIRFNSM VTFAMFHPRN
DNKAIVLQVN KQPTVEQFSP RIQTVLANDT PGSSDESASC VSYDRKGKYI IAGTGKGKLL
IYNAETLKCV AWCKQNTVQQ IRQIIVPMKS RFIITNTQDR VIRTYELEDL LHQRGQMVEA
KYKVLDMVNK AAWKNVCTDS DGLYVCGAST KAHSLYIWES NTGSLIKILH GNKGEALLDV
QWHPTRPIIL SIAQGTVSMW TQAHVENWSA FAPEFQELEE NEKYIEKENE FDMEDEDADE
DMTSKSQDAE DDIIDVVNVR PEDYLASSDE EDCNIMKPAR NLESGPLWYI PVPLDIENAD
NDQRLPDDIR NLDNLLNPAW VASAKGYELS PKSK
