ID   RBCMT_TOBAC             Reviewed;         491 AA.
AC   P94026;
DT   24-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic;
DE            EC=2.1.1.127;
DE   AltName: Full=[Ribulose-bisphosphate carboxylase]-lysine N-methyltransferase;
DE            Short=RuBisCO LSMT;
DE            Short=RuBisCO methyltransferase;
DE            Short=rbcMT;
DE   Flags: Precursor;
GN   Name=RBCMT;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=cv. KY14;
RX   PubMed=8980518; DOI=10.1007/bf00020207;
RA   Ying Z., Janney N., Houtz R.L.;
RT   "Organization and characterization of the ribulose-1,5-bisphosphate
RT   carboxylase/oxygenase large subunit epsilon N-methyltransferase gene in
RT   tobacco.";
RL   Plant Mol. Biol. 32:663-671(1996).
CC   -!- FUNCTION: Methylates 'Lys-14' of the large subunit of RuBisCO.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-lysyl-[ribulose-1,5-bisphosphate carboxylase] + 3 S-
CC         adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl-
CC         [ribulose-1,5-bisphosphate carboxylase] + 3 S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:50996, Rhea:RHEA-COMP:12858, Rhea:RHEA-
CC         COMP:12859, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:61961; EC=2.1.1.127;
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase
CC       superfamily. Plant protein-lysine LSMT methyltransferase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00916}.
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DR   EMBL; U35620; AAC49566.1; -; mRNA.
DR   EMBL; U35619; AAC49565.1; -; Genomic_DNA.
DR   PIR; T03759; T03759.
DR   RefSeq; NP_001312185.1; NM_001325256.1.
DR   AlphaFoldDB; P94026; -.
DR   SMR; P94026; -.
DR   STRING; 4097.P94026; -.
DR   GeneID; 107778201; -.
DR   KEGG; ag:AAC49566; -.
DR   KEGG; nta:107778201; -.
DR   OMA; LIPFWDF; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0030785; F:[ribulose-bisphosphate carboxylase]-lysine N-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016279; F:protein-lysine N-methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0018026; P:peptidyl-lysine monomethylation; IBA:GO_Central.
DR   CDD; cd19179; SET_RBCMT; 1.
DR   Gene3D; 3.90.1420.10; -; 1.
DR   InterPro; IPR011192; Rubisco_LSMT_MeTrfase_plant.
DR   InterPro; IPR015353; Rubisco_LSMT_subst-bd.
DR   InterPro; IPR036464; Rubisco_LSMT_subst-bd_sf.
DR   InterPro; IPR001214; SET_dom.
DR   InterPro; IPR046341; SET_dom_sf.
DR   InterPro; IPR044431; SET_RBCMT.
DR   Pfam; PF09273; Rubis-subs-bind; 1.
DR   Pfam; PF00856; SET; 1.
DR   PIRSF; PIRSF009328; RMT_SET; 1.
DR   SMART; SM00317; SET; 1.
DR   SUPFAM; SSF81822; SSF81822; 1.
DR   SUPFAM; SSF82199; SSF82199; 1.
DR   PROSITE; PS51583; SAM_MT127; 1.
DR   PROSITE; PS50280; SET; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Methyltransferase; Plastid; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase; Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Chloroplast"
FT   CHAIN           ?..491
FT                   /note="Ribulose-1,5 bisphosphate carboxylase/oxygenase
FT                   large subunit N-methyltransferase, chloroplastic"
FT                   /id="PRO_0000022200"
FT   DOMAIN          67..291
FT                   /note="SET"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00190"
SQ   SEQUENCE   491 AA;  56000 MW;  4EA7DE9E9497D286 CRC64;
     MASVFSVHPL PSSSFLCPLK TTKSRTKHHQ TFYTYQKTIL INSLQLTELD PKIPQPVQTF
     WQWLCKEGVV TTKTPVKPGI VPEGLGLVAK RDIAKGETVL QVPKRFWINP DAVAESEIGN
     VCSGLKPWIS VALFLLREKW RDDSKWKYYM DVLPKSTDST IYWSEEELSE IQGTQLLSTT
     MSVKDYVQNE FQKVEEEVIL RNKQLFPFPI TLDDFFWAFG ILRSRAFSRL RNQNLILVPF
     ADLTNHNARV TTEDHAHEVR GPAGLFSWDL LFSLRSPLKL KAGDQLFIQY DLNKSNADMA
     LDYGFIEPSS ARDAFTLTLE ISESDEFYGD KLDIAETNGI GETAYFDIKI GQSLPPTMIP
     YLRLVALGGT DAFLLESIFR NSVWGHLGLP VSRANEELIC KVVRDACKSA LSGYHTTIEE
     DEKLMEEGNL STRLQIAVGI RLGEKRVLKQ IDDIFREREL ELDELEYYGE RRLKDLGLVG
     EQGDIIFWEP K