ID   RBFA_THEMA              Reviewed;         131 AA.
AC   Q9WZV9;
DT   15-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-MAY-2022, entry version 118.
DE   RecName: Full=Ribosome-binding factor A {ECO:0000255|HAMAP-Rule:MF_00003};
GN   Name=rbfA {ECO:0000255|HAMAP-Rule:MF_00003}; OrderedLocusNames=TM_0855;
OS   Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS   / MSB8).
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX   NCBI_TaxID=243274;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX   PubMed=10360571; DOI=10.1038/20601;
RA   Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA   Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA   Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA   Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA   Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA   Smith H.O., Venter J.C., Fraser C.M.;
RT   "Evidence for lateral gene transfer between Archaea and Bacteria from
RT   genome sequence of Thermotoga maritima.";
RL   Nature 399:323-329(1999).
RN   [2]
RP   NMR ASSIGNMENTS FOR 1-120.
RC   STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX   PubMed=15692745; DOI=10.1007/s10858-004-6065-y;
RA   Grimm S.K., Woehnert J.;
RT   "NMR assignments of the cold-shock protein ribosome-binding factor A (RbfA)
RT   from Thermotoga maritima.";
RL   J. Biomol. NMR 31:73-74(2005).
RN   [3]
RP   STRUCTURE BY NMR OF 1-105, AND SUBUNIT.
RA   Serrano P., Jaudzems K., Horst R., Wilson I.A., Wuthrich K.;
RT   "Solution NMR structure of the Thermotoga maritima protein TM0855.";
RL   Submitted (JUN-2010) to the PDB data bank.
CC   -!- FUNCTION: One of several proteins that assist in the late maturation
CC       steps of the functional core of the 30S ribosomal subunit. Associates
CC       with free 30S ribosomal subunits (but not with 30S subunits that are
CC       part of 70S ribosomes or polysomes). Required for efficient processing
CC       of 16S rRNA. May interact with the 5'-terminal helix region of 16S
CC       rRNA. {ECO:0000255|HAMAP-Rule:MF_00003}.
CC   -!- SUBUNIT: Monomer (Ref.3). Binds 30S ribosomal subunits, but not 50S
CC       ribosomal subunits or 70S ribosomes. {ECO:0000255|HAMAP-Rule:MF_00003,
CC       ECO:0000305|Ref.3}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00003}.
CC   -!- SIMILARITY: Belongs to the RbfA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00003}.
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DR   EMBL; AE000512; AAD35937.1; -; Genomic_DNA.
DR   PIR; H72324; H72324.
DR   RefSeq; NP_228664.1; NC_000853.1.
DR   RefSeq; WP_004080768.1; NZ_CP011107.1.
DR   PDB; 2KZF; NMR; -; A=1-105.
DR   PDBsum; 2KZF; -.
DR   AlphaFoldDB; Q9WZV9; -.
DR   BMRB; Q9WZV9; -.
DR   SMR; Q9WZV9; -.
DR   STRING; 243274.THEMA_00360; -.
DR   EnsemblBacteria; AAD35937; AAD35937; TM_0855.
DR   KEGG; tma:TM0855; -.
DR   eggNOG; COG0858; Bacteria.
DR   InParanoid; Q9WZV9; -.
DR   OMA; GDLQHCK; -.
DR   OrthoDB; 1971380at2; -.
DR   EvolutionaryTrace; Q9WZV9; -.
DR   Proteomes; UP000008183; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0043024; F:ribosomal small subunit binding; IBA:GO_Central.
DR   GO; GO:0030490; P:maturation of SSU-rRNA; IEA:UniProtKB-UniRule.
DR   GO; GO:0042254; P:ribosome biogenesis; IBA:GO_Central.
DR   Gene3D; 3.30.300.20; -; 1.
DR   HAMAP; MF_00003; RbfA; 1.
DR   InterPro; IPR015946; KH_dom-like_a/b.
DR   InterPro; IPR000238; RbfA.
DR   InterPro; IPR023799; RbfA_dom_sf.
DR   InterPro; IPR020053; Ribosome-bd_factorA_CS.
DR   PANTHER; PTHR33515; PTHR33515; 1.
DR   Pfam; PF02033; RBFA; 1.
DR   SUPFAM; SSF89919; SSF89919; 1.
DR   TIGRFAMs; TIGR00082; rbfA; 1.
DR   PROSITE; PS01319; RBFA; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Reference proteome; Ribosome biogenesis.
FT   CHAIN           1..131
FT                   /note="Ribosome-binding factor A"
FT                   /id="PRO_0000102758"
FT   TURN            5..7
FT                   /evidence="ECO:0007829|PDB:2KZF"
FT   HELIX           8..23
FT                   /evidence="ECO:0007829|PDB:2KZF"
FT   HELIX           32..34
FT                   /evidence="ECO:0007829|PDB:2KZF"
FT   STRAND          37..42
FT                   /evidence="ECO:0007829|PDB:2KZF"
FT   STRAND          48..53
FT                   /evidence="ECO:0007829|PDB:2KZF"
FT   HELIX           59..83
FT                   /evidence="ECO:0007829|PDB:2KZF"
FT   STRAND          86..88
FT                   /evidence="ECO:0007829|PDB:2KZF"
FT   STRAND          91..97
FT                   /evidence="ECO:0007829|PDB:2KZF"
SQ   SEQUENCE   131 AA;  15547 MW;  C1C58F7E8C893763 CRC64;
     MNPAYRKAML ESEIQKLLME ALQQLRDPRL KKDFVTFSRV ELSKDKRYAD VYVSFLGTPE
     ERKETVEILN RAKGFFRTFI AKNLRLYVAP EIRFYEDKGI EASVKVHQLL VQLGYDPLKD
     KEKKEEDKEE E