ID   RBFA_THET8              Reviewed;          95 AA.
AC   Q5SJV1;
DT   15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Ribosome-binding factor A {ECO:0000303|PubMed:17996707};
DE   AltName: Full=30S ribosome-binding protein;
GN   Name=rbfA {ECO:0000303|PubMed:17996707}; OrderedLocusNames=TTHA0907;
OS   Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC   Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX   NCBI_TaxID=300852;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27634 / DSM 579 / HB8;
RA   Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA   Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT   "Complete genome sequence of Thermus thermophilus HB8.";
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0007744|PDB:2DYJ, ECO:0007744|PDB:2R1C}
RP   X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS), STRUCTURE BY ELECTRON MICROSCOPY
RP   (12.5 ANGSTROMS) ON THE 30S RIBOSOMAL SUBUNIT, FUNCTION, SUBUNIT,
RP   SUBCELLULAR LOCATION, AND RRNA-BINDING.
RC   STRAIN=ATCC 27634 / DSM 579 / HB8;
RX   PubMed=17996707; DOI=10.1016/j.molcel.2007.08.026;
RA   Datta P.P., Wilson D.N., Kawazoe M., Swami N.K., Kaminishi T., Sharma M.R.,
RA   Booth T.M., Takemoto C., Fucini P., Yokoyama S., Agrawal R.K.;
RT   "Structural aspects of RbfA action during small ribosomal subunit
RT   assembly.";
RL   Mol. Cell 28:434-445(2007).
CC   -!- FUNCTION: One of several proteins that assist in the late maturation
CC       steps of the functional core of the 30S ribosomal subunit. Associates
CC       with free 30S ribosomal subunits (but not with 30S subunits that are
CC       part of 70S ribosomes or polysomes) (PubMed:17996707). Required for
CC       efficient processing of 16S rRNA. Probably interacts with the 5'-
CC       terminal helix region of 16S rRNA, bringing together different domains
CC       of the 30S ribosomal subunit which aids assembly (PubMed:17996707).
CC       {ECO:0000269|PubMed:17996707}.
CC   -!- SUBUNIT: Interacts with the 30S ribosomal subunit as a monomer, binding
CC       in a position overlapping the sites of the A and P site tRNAs, and
CC       displacing segments of the 16S rRNA. Probably contacts 16S rRNA and
CC       ribosomal protein S9 and S13. {ECO:0000269|PubMed:17996707}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:17996707}.
CC       Note=Binds to the mature 30S ribosomal subunit.
CC       {ECO:0000269|PubMed:17996707}.
CC   -!- SIMILARITY: Belongs to the RbfA family. {ECO:0000305}.
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DR   EMBL; AP008226; BAD70730.1; -; Genomic_DNA.
DR   RefSeq; WP_011172996.1; NC_006461.1.
DR   RefSeq; YP_144173.1; NC_006461.1.
DR   PDB; 2DYJ; X-ray; 1.84 A; A/B=1-95.
DR   PDB; 2R1C; EM; 12.50 A; A=1-95.
DR   PDBsum; 2DYJ; -.
DR   PDBsum; 2R1C; -.
DR   AlphaFoldDB; Q5SJV1; -.
DR   SMR; Q5SJV1; -.
DR   STRING; 300852.55772289; -.
DR   EnsemblBacteria; BAD70730; BAD70730; BAD70730.
DR   GeneID; 3169952; -.
DR   KEGG; ttj:TTHA0907; -.
DR   PATRIC; fig|300852.9.peg.899; -.
DR   eggNOG; COG0858; Bacteria.
DR   HOGENOM; CLU_089475_6_4_0; -.
DR   OMA; RTPTLEF; -.
DR   EvolutionaryTrace; Q5SJV1; -.
DR   Proteomes; UP000000532; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0030490; P:maturation of SSU-rRNA; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.300.20; -; 1.
DR   HAMAP; MF_00003; RbfA; 1.
DR   InterPro; IPR015946; KH_dom-like_a/b.
DR   InterPro; IPR000238; RbfA.
DR   InterPro; IPR023799; RbfA_dom_sf.
DR   Pfam; PF02033; RBFA; 1.
DR   SUPFAM; SSF89919; SSF89919; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Reference proteome; Ribosome biogenesis;
KW   RNA-binding; rRNA-binding.
FT   CHAIN           1..95
FT                   /note="Ribosome-binding factor A"
FT                   /id="PRO_0000438983"
FT   HELIX           5..21
FT                   /evidence="ECO:0007829|PDB:2DYJ"
FT   HELIX           26..28
FT                   /evidence="ECO:0007829|PDB:2DYJ"
FT   STRAND          32..38
FT                   /evidence="ECO:0007829|PDB:2DYJ"
FT   STRAND          42..50
FT                   /evidence="ECO:0007829|PDB:2DYJ"
FT   HELIX           55..64
FT                   /evidence="ECO:0007829|PDB:2DYJ"
FT   HELIX           66..74
FT                   /evidence="ECO:0007829|PDB:2DYJ"
FT   STRAND          84..89
FT                   /evidence="ECO:0007829|PDB:2DYJ"
FT   HELIX           90..92
FT                   /evidence="ECO:0007829|PDB:2DYJ"
SQ   SEQUENCE   95 AA;  10857 MW;  2807A8979E74A3C9 CRC64;
     MAYGKAHLEA QLKRALAEEI QALEDPRLFL LTVEAVRLSK DGSVLSVYVE AFREEEGALR
     ALSRAERRLV AALARRVRMR RLPRLEFLPW RASPA