RBL14_ARATH
ID RBL14_ARATH Reviewed; 334 AA.
AC Q8RXW0; A8MS64; Q27GK6; Q682Z5;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Rhomboid-like protein 14, mitochondrial {ECO:0000303|PubMed:17181860};
DE Short=AtRBL14 {ECO:0000303|PubMed:17181860};
DE EC=3.4.21.- {ECO:0000305};
DE Flags: Precursor;
GN Name=RBL14 {ECO:0000303|PubMed:17181860};
GN Synonyms=RBL10 {ECO:0000303|PubMed:17938163};
GN OrderedLocusNames=At3g17611 {ECO:0000312|Araport:AT3G17611};
GN ORFNames=MKP6.17 {ECO:0000312|EMBL:BAB02051.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 196-334.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16895613; DOI=10.1186/1471-2164-7-200;
RA Tripathi L.P., Sowdhamini R.;
RT "Cross genome comparisons of serine proteases in Arabidopsis and rice.";
RL BMC Genomics 7:200-200(2006).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17181860; DOI=10.1186/1471-2229-6-30;
RA Garcia-Lorenzo M., Sjodin A., Jansson S., Funk C.;
RT "Protease gene families in Populus and Arabidopsis.";
RL BMC Plant Biol. 6:30-30(2006).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17938163; DOI=10.1101/gr.6425307;
RA Lemberg M.K., Freeman M.;
RT "Functional and evolutionary implications of enhanced genomic analysis of
RT rhomboid intramembrane proteases.";
RL Genome Res. 17:1634-1646(2007).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=18543065; DOI=10.1007/s11103-008-9359-8;
RA Kmiec-Wisniewska B., Krumpe K., Urantowka A., Sakamoto W., Pratje E.,
RA Janska H.;
RT "Plant mitochondrial rhomboid, AtRBL12, has different substrate specificity
RT from its yeast counterpart.";
RL Plant Mol. Biol. 68:159-171(2008).
RN [9]
RP REVIEW.
RX PubMed=22007993; DOI=10.1111/j.1399-3054.2011.01532.x;
RA Knopf R.R., Adam Z.;
RT "Rhomboid proteases in plants - still in square one?";
RL Physiol. Plantarum 145:41-51(2012).
CC -!- FUNCTION: Probable rhomboid-type serine protease that catalyzes
CC intramembrane proteolysis. May function in the heat-shock response
CC pathway. {ECO:0000303|PubMed:22007993}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}. Note=Might be neither mitochondrial nor
CC chloroplastic. {ECO:0000305|PubMed:18543065}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8RXW0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8RXW0-2; Sequence=VSP_057724;
CC Name=3;
CC IsoId=Q8RXW0-3; Sequence=VSP_057723;
CC -!- SIMILARITY: Belongs to the peptidase S54 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB02051.1; Type=Erroneous gene model prediction; Note=The predicted gene At3g17610 has been split into 2 genes: At3g17609 and At3g17611.; Evidence={ECO:0000305};
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DR EMBL; AB022219; BAB02051.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE75978.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75979.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75980.1; -; Genomic_DNA.
DR EMBL; AY080647; AAL85014.1; -; mRNA.
DR EMBL; AY117194; AAM51269.1; -; mRNA.
DR EMBL; AK175222; BAD42985.1; -; mRNA.
DR RefSeq; NP_001078170.1; NM_001084701.1. [Q8RXW0-3]
DR RefSeq; NP_850606.1; NM_180275.3. [Q8RXW0-1]
DR RefSeq; NP_974329.1; NM_202600.1. [Q8RXW0-2]
DR AlphaFoldDB; Q8RXW0; -.
DR BioGRID; 6361; 6.
DR IntAct; Q8RXW0; 6.
DR STRING; 3702.AT3G17611.1; -.
DR PaxDb; Q8RXW0; -.
DR PRIDE; Q8RXW0; -.
DR ProteomicsDB; 236473; -. [Q8RXW0-1]
DR EnsemblPlants; AT3G17611.1; AT3G17611.1; AT3G17611. [Q8RXW0-1]
DR EnsemblPlants; AT3G17611.2; AT3G17611.2; AT3G17611. [Q8RXW0-2]
DR EnsemblPlants; AT3G17611.3; AT3G17611.3; AT3G17611. [Q8RXW0-3]
DR GeneID; 821028; -.
DR Gramene; AT3G17611.1; AT3G17611.1; AT3G17611. [Q8RXW0-1]
DR Gramene; AT3G17611.2; AT3G17611.2; AT3G17611. [Q8RXW0-2]
DR Gramene; AT3G17611.3; AT3G17611.3; AT3G17611. [Q8RXW0-3]
DR KEGG; ath:AT3G17611; -.
DR Araport; AT3G17611; -.
DR TAIR; locus:1005716534; AT3G17611.
DR eggNOG; KOG2632; Eukaryota.
DR HOGENOM; CLU_056643_0_0_1; -.
DR InParanoid; Q8RXW0; -.
DR OMA; HFFFNMS; -.
DR PhylomeDB; Q8RXW0; -.
DR PRO; PR:Q8RXW0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8RXW0; baseline and differential.
DR Genevisible; Q8RXW0; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1540.10; -; 1.
DR InterPro; IPR022764; Peptidase_S54_rhomboid_dom.
DR InterPro; IPR035952; Rhomboid-like_sf.
DR InterPro; IPR001876; Znf_RanBP2.
DR InterPro; IPR036443; Znf_RanBP2_sf.
DR Pfam; PF01694; Rhomboid; 1.
DR Pfam; PF00641; zf-RanBP; 1.
DR SMART; SM00547; ZnF_RBZ; 1.
DR SUPFAM; SSF144091; SSF144091; 1.
DR SUPFAM; SSF90209; SSF90209; 1.
DR PROSITE; PS01358; ZF_RANBP2_1; 1.
DR PROSITE; PS50199; ZF_RANBP2_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Hydrolase; Membrane; Metal-binding; Mitochondrion;
KW Protease; Reference proteome; Transit peptide; Transmembrane;
KW Transmembrane helix; Zinc; Zinc-finger.
FT TRANSIT 1..87
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 88..334
FT /note="Rhomboid-like protein 14, mitochondrial"
FT /id="PRO_0000223928"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ZN_FING 273..302
FT /note="RanBP2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00322"
FT ACT_SITE 156
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:P54493"
FT ACT_SITE 206
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:P54493"
FT VAR_SEQ 1..161
FT /note="Missing (in isoform 3)"
FT /id="VSP_057723"
FT VAR_SEQ 1..95
FT /note="Missing (in isoform 2)"
FT /id="VSP_057724"
SQ SEQUENCE 334 AA; 37150 MW; 8889A08D64484AD6 CRC64;
MENFGEGRRS GGGMLPLLAL SAVAEYYRLP WKPPVTASLL AANTLVYLRP AFIDPVIPHI
SEVWFNPHLI FKHKDLKRLF LSAFYHVNEP HLVYNMMSLL WKGIKLETSM GSSEFASMVF
TLIGMSQGVT LLLAKSLLLL FDYDRAYYNE YAVGFSGVLF AMKVVLNSQA EDYSSVYGIL
VPTKYAAWAE LILVQMFVPN ASFLGHLGGI LAGIIYLKLK GSYSGSDPVT MAVRGVSRLV
TWPLRFLNGM VRSRRRRITG RGRVGRGQTG IAGPGIWRCQ SCTYDNSGWL SACEMCGSGR
ARGNGWSLNQ GPALSSSNDL PLDELRRRRV ERFS
