RBL16_ARATH
ID RBL16_ARATH Reviewed; 322 AA.
AC Q84WG3; F4HTU5; Q9C6A2;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Rhomboid-like protein 16, chloroplastic {ECO:0000303|PubMed:16895613};
DE Short=AtRBL16 {ECO:0000303|PubMed:16895613};
DE Flags: Precursor;
GN Name=RBL16 {ECO:0000303|PubMed:16895613};
GN OrderedLocusNames=At1g74130 {ECO:0000312|Araport:AT1G74130};
GN ORFNames=F9E11.2 {ECO:0000312|EMBL:AAG51877.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAO41899.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16895613; DOI=10.1186/1471-2164-7-200;
RA Tripathi L.P., Sowdhamini R.;
RT "Cross genome comparisons of serine proteases in Arabidopsis and rice.";
RL BMC Genomics 7:200-200(2006).
RN [5]
RP GENE FAMILY.
RX PubMed=17938163; DOI=10.1101/gr.6425307;
RA Lemberg M.K., Freeman M.;
RT "Functional and evolutionary implications of enhanced genomic analysis of
RT rhomboid intramembrane proteases.";
RL Genome Res. 17:1634-1646(2007).
RN [6]
RP FUNCTION.
RX PubMed=17327256; DOI=10.1093/pcp/pcm031;
RA Karakasis K., Taylor D., Ko K.;
RT "Uncovering a link between a plastid translocon component and rhomboid
RT proteases using yeast mitochondria-based assays.";
RL Plant Cell Physiol. 48:655-661(2007).
CC -!- FUNCTION: Rhomboid-type serine protease that catalyzes intramembrane
CC proteolysis. May cleave the plastid translocon component Tic40.
CC {ECO:0000303|PubMed:17327256}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000255};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q84WG3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q84WG3-2; Sequence=VSP_057733, VSP_057734;
CC -!- SIMILARITY: Belongs to the peptidase S54 family. {ECO:0000305}.
CC -!- CAUTION: Might be an inactive rhomboid-type serine protease due to
CC mismatches with the consensus active sites.
CC {ECO:0000303|PubMed:17938163}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51877.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC079678; AAG51877.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35553.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35554.1; -; Genomic_DNA.
DR EMBL; BT003849; AAO41899.1; -; mRNA.
DR PIR; D96769; D96769.
DR RefSeq; NP_177553.3; NM_106073.5. [Q84WG3-1]
DR RefSeq; NP_974141.1; NM_202412.1. [Q84WG3-2]
DR AlphaFoldDB; Q84WG3; -.
DR STRING; 3702.AT1G74130.1; -.
DR MEROPS; S54.A07; -.
DR PaxDb; Q84WG3; -.
DR PRIDE; Q84WG3; -.
DR EnsemblPlants; AT1G74130.1; AT1G74130.1; AT1G74130. [Q84WG3-1]
DR EnsemblPlants; AT1G74130.2; AT1G74130.2; AT1G74130. [Q84WG3-2]
DR GeneID; 843753; -.
DR Gramene; AT1G74130.1; AT1G74130.1; AT1G74130. [Q84WG3-1]
DR Gramene; AT1G74130.2; AT1G74130.2; AT1G74130. [Q84WG3-2]
DR KEGG; ath:AT1G74130; -.
DR Araport; AT1G74130; -.
DR TAIR; locus:2037333; AT1G74130.
DR eggNOG; KOG2980; Eukaryota.
DR HOGENOM; CLU_048192_0_0_1; -.
DR InParanoid; Q84WG3; -.
DR OMA; FSHWSQS; -.
DR OrthoDB; 1554324at2759; -.
DR PhylomeDB; Q84WG3; -.
DR PRO; PR:Q84WG3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q84WG3; baseline and differential.
DR Genevisible; Q84WG3; AT.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1540.10; -; 1.
DR InterPro; IPR022764; Peptidase_S54_rhomboid_dom.
DR InterPro; IPR035952; Rhomboid-like_sf.
DR Pfam; PF01694; Rhomboid; 1.
DR SUPFAM; SSF144091; SSF144091; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Hydrolase; Membrane; Plastid; Protease;
KW Reference proteome; Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..52
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 53..322
FT /note="Rhomboid-like protein 16, chloroplastic"
FT /id="PRO_0000433335"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..258
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 290..296
FT /note="GKNNNTL -> VGKTTIP (in isoform 2)"
FT /id="VSP_057733"
FT VAR_SEQ 297..322
FT /note="Missing (in isoform 2)"
FT /id="VSP_057734"
SQ SEQUENCE 322 AA; 35451 MW; EF1E177F363B2769 CRC64;
MHAIFCRRVA VGCSSPQLTK LVTKQASQSR HSLSHLLPFD LSSRFVPPYV VSRSARVHGF
FAGKLGNTNL KLKFGNVMES RAGFFSSELP SHGFESGGFT GFQKRGWKSW INGANGVVFG
LVIANAAVFT MWRVLGKDNM WMVKNFMLSR YSFMTGRIHT LITSGFSHVG ATHIILNMMG
LCYFGARIAR SFGPRYLLKL YFAGALGGSV FFLSSHALSV ISLKGQRVVP KDQLKVPIGK
LGANGPVYAI TLLDMLLYPK VTTYFGLMLR VPVFAGIYSL GLNIIKMLEG KNNNTLTSLD
QLGGVVVAVI AWARIRKGRF CY
