RBL1_ARATH
ID RBL1_ARATH Reviewed; 389 AA.
AC Q0WQX7; O81073; Q3C2H8;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=RHOMBOID-like protein 1 {ECO:0000303|PubMed:16223493, ECO:0000303|PubMed:17181860};
DE Short=AtRBL1 {ECO:0000303|PubMed:16223493, ECO:0000303|PubMed:17181860};
DE EC=3.4.21.- {ECO:0000305};
GN Name=RBL1 {ECO:0000303|PubMed:16223493, ECO:0000303|PubMed:17181860};
GN OrderedLocusNames=At2g29050 {ECO:0000312|Araport:AT2G29050};
GN ORFNames=T9I4.13 {ECO:0000312|EMBL:AAC33231.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:BAF00472.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), GENE FAMILY, NOMENCLATURE, TISSUE
RP SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=16223493; DOI=10.1016/j.febslet.2005.09.049;
RA Kanaoka M.M., Urban S., Freeman M., Okada K.;
RT "An Arabidopsis Rhomboid homolog is an intramembrane protease in plants.";
RL FEBS Lett. 579:5723-5728(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16895613; DOI=10.1186/1471-2164-7-200;
RA Tripathi L.P., Sowdhamini R.;
RT "Cross genome comparisons of serine proteases in Arabidopsis and rice.";
RL BMC Genomics 7:200-200(2006).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17181860; DOI=10.1186/1471-2229-6-30;
RA Garcia-Lorenzo M., Sjodin A., Jansson S., Funk C.;
RT "Protease gene families in Populus and Arabidopsis.";
RL BMC Plant Biol. 6:30-30(2006).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17938163; DOI=10.1101/gr.6425307;
RA Lemberg M.K., Freeman M.;
RT "Functional and evolutionary implications of enhanced genomic analysis of
RT rhomboid intramembrane proteases.";
RL Genome Res. 17:1634-1646(2007).
RN [8]
RP REVIEW.
RX PubMed=22007993; DOI=10.1111/j.1399-3054.2011.01532.x;
RA Knopf R.R., Adam Z.;
RT "Rhomboid proteases in plants - still in square one?";
RL Physiol. Plantarum 145:41-51(2012).
CC -!- FUNCTION: Probable rhomboid-type serine protease that catalyzes
CC intramembrane proteolysis. Unable to cleave the Drosophila protein
CC Spitz. {ECO:0000269|PubMed:16223493}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:16223493}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0WQX7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0WQX7-2; Sequence=VSP_057727, VSP_057728, VSP_057729;
CC -!- TISSUE SPECIFICITY: Expressed in roots, seedlings, leaves, stems and
CC flowers. {ECO:0000269|PubMed:16223493}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000305|PubMed:16223493}.
CC -!- SIMILARITY: Belongs to the peptidase S54 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC33231.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB195672; BAE46872.1; -; mRNA.
DR EMBL; AC005315; AAC33231.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC08202.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08203.1; -; Genomic_DNA.
DR EMBL; AK228551; BAF00472.1; -; mRNA.
DR PIR; T02735; T02735.
DR RefSeq; NP_001077973.1; NM_001084504.1. [Q0WQX7-2]
DR RefSeq; NP_180469.3; NM_128462.4. [Q0WQX7-1]
DR AlphaFoldDB; Q0WQX7; -.
DR STRING; 3702.AT2G29050.1; -.
DR MEROPS; S54.A05; -.
DR PaxDb; Q0WQX7; -.
DR PRIDE; Q0WQX7; -.
DR ProteomicsDB; 225972; -. [Q0WQX7-1]
DR EnsemblPlants; AT2G29050.1; AT2G29050.1; AT2G29050. [Q0WQX7-1]
DR EnsemblPlants; AT2G29050.2; AT2G29050.2; AT2G29050. [Q0WQX7-2]
DR GeneID; 817453; -.
DR Gramene; AT2G29050.1; AT2G29050.1; AT2G29050. [Q0WQX7-1]
DR Gramene; AT2G29050.2; AT2G29050.2; AT2G29050. [Q0WQX7-2]
DR KEGG; ath:AT2G29050; -.
DR Araport; AT2G29050; -.
DR TAIR; locus:2066102; AT2G29050.
DR eggNOG; KOG2289; Eukaryota.
DR InParanoid; Q0WQX7; -.
DR OMA; CTEARCA; -.
DR OrthoDB; 1253228at2759; -.
DR PhylomeDB; Q0WQX7; -.
DR PRO; PR:Q0WQX7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q0WQX7; baseline and differential.
DR Genevisible; Q0WQX7; AT.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1540.10; -; 1.
DR InterPro; IPR002610; Peptidase_S54_rhomboid.
DR InterPro; IPR022764; Peptidase_S54_rhomboid_dom.
DR InterPro; IPR035952; Rhomboid-like_sf.
DR PANTHER; PTHR22936; PTHR22936; 1.
DR Pfam; PF01694; Rhomboid; 1.
DR SUPFAM; SSF144091; SSF144091; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Golgi apparatus; Hydrolase; Membrane; Protease;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..389
FT /note="RHOMBOID-like protein 1"
FT /id="PRO_0000433322"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 196
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:P54493"
FT ACT_SITE 248
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:P54493"
FT VAR_SEQ 220
FT /note="K -> KVAKSSLVKQAALSMNDVSIMSLVFLHLQ (in isoform 2)"
FT /id="VSP_057727"
FT VAR_SEQ 310..318
FT /note="AGLVVLLRG -> SFSFIMITK (in isoform 2)"
FT /id="VSP_057728"
FT VAR_SEQ 319..389
FT /note="Missing (in isoform 2)"
FT /id="VSP_057729"
SQ SEQUENCE 389 AA; 43360 MW; 474FE497A127506E CRC64;
MARDRREGLE IKVVNPPAAA TNNVAVETSP ATATRRRRQQ QRASFAEFRP FKLWFPWLVP
AIVVANIALF AISMFINNCP KNSAYCLARF LGRFAFQPMK ENPLLGPSSL TLEKMGALDV
SMVVHKHEVW RLFTCIWLHA GVFHVLANML SLIFIGIRLE QEFGFVRIGL LYMISGFGGS
LLSSLFNRAG ISVGASGALF GLLGAMLSEL LTNWTIYANK FAALLTLIFI IAINLAVGIL
PHVDNFAHLG GFTSGFLLGF VFLIRPQYGY FNQRNNPRGY AAPSAKSKHK PYQYVLWITS
LVLLIAGYTA GLVVLLRGTD LNKHCSWCHY LSCIPTSLWS CKSQNVYCES SQIGQQMNLT
CITNGKTEMY KLSNDIPSRI QQLCSQLCR
