RBL4_ARATH
ID RBL4_ARATH Reviewed; 394 AA.
AC F4JBM4; A1A6L3; Q9M348;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=RHOMBOID-like protein 4 {ECO:0000303|PubMed:16223493, ECO:0000303|PubMed:17181860};
DE Short=AtRBL4 {ECO:0000303|PubMed:16223493, ECO:0000303|PubMed:17181860};
DE EC=3.4.21.- {ECO:0000305};
GN Name=RBL4 {ECO:0000303|PubMed:16223493, ECO:0000303|PubMed:17181860};
GN OrderedLocusNames=At3g53780 {ECO:0000312|Araport:AT3G53780};
GN ORFNames=F5K20.80 {ECO:0000312|EMBL:CAB88340.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=16223493; DOI=10.1016/j.febslet.2005.09.049;
RA Kanaoka M.M., Urban S., Freeman M., Okada K.;
RT "An Arabidopsis Rhomboid homolog is an intramembrane protease in plants.";
RL FEBS Lett. 579:5723-5728(2005).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16895613; DOI=10.1186/1471-2164-7-200;
RA Tripathi L.P., Sowdhamini R.;
RT "Cross genome comparisons of serine proteases in Arabidopsis and rice.";
RL BMC Genomics 7:200-200(2006).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17181860; DOI=10.1186/1471-2229-6-30;
RA Garcia-Lorenzo M., Sjodin A., Jansson S., Funk C.;
RT "Protease gene families in Populus and Arabidopsis.";
RL BMC Plant Biol. 6:30-30(2006).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17938163; DOI=10.1101/gr.6425307;
RA Lemberg M.K., Freeman M.;
RT "Functional and evolutionary implications of enhanced genomic analysis of
RT rhomboid intramembrane proteases.";
RL Genome Res. 17:1634-1646(2007).
RN [8]
RP REVIEW.
RX PubMed=22007993; DOI=10.1111/j.1399-3054.2011.01532.x;
RA Knopf R.R., Adam Z.;
RT "Rhomboid proteases in plants - still in square one?";
RL Physiol. Plantarum 145:41-51(2012).
CC -!- FUNCTION: Probable rhomboid-type serine protease that catalyzes
CC intramembrane proteolysis. {ECO:0000303|PubMed:22007993}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4JBM4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4JBM4-2; Sequence=VSP_057731;
CC -!- SIMILARITY: Belongs to the peptidase S54 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB88340.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL132960; CAB88340.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE79142.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79143.1; -; Genomic_DNA.
DR EMBL; BT029533; ABL66789.1; -; mRNA.
DR PIR; T45918; T45918.
DR RefSeq; NP_566989.1; NM_115238.2. [F4JBM4-2]
DR RefSeq; NP_850698.1; NM_180367.2. [F4JBM4-1]
DR AlphaFoldDB; F4JBM4; -.
DR STRING; 3702.AT3G53780.2; -.
DR MEROPS; S54.A06; -.
DR PaxDb; F4JBM4; -.
DR PRIDE; F4JBM4; -.
DR ProteomicsDB; 236524; -. [F4JBM4-1]
DR EnsemblPlants; AT3G53780.1; AT3G53780.1; AT3G53780. [F4JBM4-2]
DR EnsemblPlants; AT3G53780.2; AT3G53780.2; AT3G53780. [F4JBM4-1]
DR GeneID; 824545; -.
DR Gramene; AT3G53780.1; AT3G53780.1; AT3G53780. [F4JBM4-2]
DR Gramene; AT3G53780.2; AT3G53780.2; AT3G53780. [F4JBM4-1]
DR KEGG; ath:AT3G53780; -.
DR Araport; AT3G53780; -.
DR TAIR; locus:2084465; AT3G53780.
DR eggNOG; KOG2289; Eukaryota.
DR InParanoid; F4JBM4; -.
DR OrthoDB; 1253228at2759; -.
DR PRO; PR:F4JBM4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4JBM4; baseline and differential.
DR Genevisible; F4JBM4; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1540.10; -; 1.
DR InterPro; IPR002610; Peptidase_S54_rhomboid.
DR InterPro; IPR022764; Peptidase_S54_rhomboid_dom.
DR InterPro; IPR035952; Rhomboid-like_sf.
DR PANTHER; PTHR22936; PTHR22936; 1.
DR Pfam; PF01694; Rhomboid; 1.
DR SUPFAM; SSF144091; SSF144091; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Hydrolase; Membrane; Protease; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..394
FT /note="RHOMBOID-like protein 4"
FT /id="PRO_0000433325"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..195
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 231..251
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..23
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..51
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 206
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:P54493"
FT ACT_SITE 258
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:P54493"
FT VAR_SEQ 1..124
FT /note="Missing (in isoform 2)"
FT /id="VSP_057731"
SQ SEQUENCE 394 AA; 43508 MW; B562075ACE518E35 CRC64;
MGEKDSETAP IWGKTRERER SNNNNIQPMD LESSSSVSGQ QRSLTQSRSS YEERGRGVKE
FRSWFPWLIP CFVVANVAVF VITMYVNNCP KKSGDCFADF LGRFSFQNTR ENPLLGPSSL
TLQTMGGLDV KKVVKGDEGW RLLSCNWLHG GVVHLLMNML TLLFIGIRME REFGFIRIGL
LYLISGFGGS ILSALFLRSN ISVGASGAVF GLLGGMLSEI FINWTIYSNK VVTIVTLVLI
VAVNLGLGVL PGVDNFAHIG GFATGFLLGF VLLIRPHYGW INQRNGPGAK PHRFKIYQGI
LWTISLLILV AGFIVGLISL FNNVDGNEHC SWCHYLSCVP TSKWSCNREP ASCTTTQLGN
QLSMTCLRNG KSASYILANP SDSRINSLCV QLCR
