RBL5_ARATH
ID RBL5_ARATH Reviewed; 309 AA.
AC Q9SSR0;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=RHOMBOID-like protein 5 {ECO:0000303|PubMed:16223493, ECO:0000303|PubMed:17181860};
DE Short=AtRBL5 {ECO:0000303|PubMed:16223493, ECO:0000303|PubMed:17181860};
DE EC=3.4.21.- {ECO:0000305};
GN Name=RBL5 {ECO:0000303|PubMed:16223493, ECO:0000303|PubMed:17181860};
GN OrderedLocusNames=At1g52580 {ECO:0000312|Araport:AT1G52580};
GN ORFNames=F6D8.20 {ECO:0000312|EMBL:AAD55606.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=16223493; DOI=10.1016/j.febslet.2005.09.049;
RA Kanaoka M.M., Urban S., Freeman M., Okada K.;
RT "An Arabidopsis Rhomboid homolog is an intramembrane protease in plants.";
RL FEBS Lett. 579:5723-5728(2005).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16895613; DOI=10.1186/1471-2164-7-200;
RA Tripathi L.P., Sowdhamini R.;
RT "Cross genome comparisons of serine proteases in Arabidopsis and rice.";
RL BMC Genomics 7:200-200(2006).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17181860; DOI=10.1186/1471-2229-6-30;
RA Garcia-Lorenzo M., Sjodin A., Jansson S., Funk C.;
RT "Protease gene families in Populus and Arabidopsis.";
RL BMC Plant Biol. 6:30-30(2006).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17938163; DOI=10.1101/gr.6425307;
RA Lemberg M.K., Freeman M.;
RT "Functional and evolutionary implications of enhanced genomic analysis of
RT rhomboid intramembrane proteases.";
RL Genome Res. 17:1634-1646(2007).
RN [7]
RP REVIEW.
RX PubMed=22007993; DOI=10.1111/j.1399-3054.2011.01532.x;
RA Knopf R.R., Adam Z.;
RT "Rhomboid proteases in plants - still in square one?";
RL Physiol. Plantarum 145:41-51(2012).
CC -!- FUNCTION: Probable rhomboid-type serine protease that catalyzes
CC intramembrane proteolysis. May function in reproductive organs
CC maturation. {ECO:0000303|PubMed:22007993}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the peptidase S54 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC008016; AAD55606.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32826.1; -; Genomic_DNA.
DR PIR; E96566; E96566.
DR RefSeq; NP_175667.1; NM_104136.3.
DR AlphaFoldDB; Q9SSR0; -.
DR STRING; 3702.AT1G52580.1; -.
DR MEROPS; S54.A01; -.
DR PaxDb; Q9SSR0; -.
DR PRIDE; Q9SSR0; -.
DR ProteomicsDB; 225969; -.
DR EnsemblPlants; AT1G52580.1; AT1G52580.1; AT1G52580.
DR GeneID; 841690; -.
DR Gramene; AT1G52580.1; AT1G52580.1; AT1G52580.
DR KEGG; ath:AT1G52580; -.
DR Araport; AT1G52580; -.
DR TAIR; locus:2035190; AT1G52580.
DR eggNOG; KOG2289; Eukaryota.
DR HOGENOM; CLU_011531_0_0_1; -.
DR InParanoid; Q9SSR0; -.
DR OMA; HVDNSAH; -.
DR OrthoDB; 1253228at2759; -.
DR PhylomeDB; Q9SSR0; -.
DR PRO; PR:Q9SSR0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SSR0; baseline and differential.
DR Genevisible; Q9SSR0; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1540.10; -; 1.
DR InterPro; IPR002610; Peptidase_S54_rhomboid.
DR InterPro; IPR022764; Peptidase_S54_rhomboid_dom.
DR InterPro; IPR035952; Rhomboid-like_sf.
DR PANTHER; PTHR22936; PTHR22936; 1.
DR Pfam; PF01694; Rhomboid; 1.
DR SUPFAM; SSF144091; SSF144091; 1.
PE 3: Inferred from homology;
KW Hydrolase; Membrane; Protease; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..309
FT /note="RHOMBOID-like protein 5"
FT /id="PRO_0000433326"
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 175
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:P54493"
FT ACT_SITE 227
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:P54493"
SQ SEQUENCE 309 AA; 34555 MW; B30B58B967C614A0 CRC64;
MGKRPPIPPD IENGPPPPAR PHFRPPIPVP WVAWLVPLIL AANFVTFATT MYVNDCPARS
DECLLFDVLG RLSFQPIKEN MLLGPSIPTL RKLGALERRL VEEGERWRLI SCIWLHGGFL
HLMANMISLM CIGMRLEQEF GFMRIGALYV ISGLGGSLVS CLTDSQGERV SVGASGALFG
LLGAMLSELI TNWTIYENKC TALMTLILII VLNLSVGFLP RVDNSAHFGG FLAGFFLGFV
LLLRPQYGYV NPKYIPPGYD MKHKKSKHKC YQHIFRFTSL AILLAGFIAG YTKLLREHTI
QSMPFRDFN
