RBLOT_ARATH
ID RBLOT_ARATH Reviewed; 594 AA.
AC Q0WVH0; Q8LDH9; Q9M2S8;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Protein REBELOTE {ECO:0000303|PubMed:30338215};
GN Name=RBL {ECO:0000303|PubMed:30338215};
GN OrderedLocusNames=At3g55510 {ECO:0000312|Araport:AT3G55510};
GN ORFNames=T22E16.170 {ECO:0000312|EMBL:CAB75909.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, MUTAGENESIS OF ALA-8, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY,
RP AND SUBCELLULAR LOCATION.
RX PubMed=18441215; DOI=10.1105/tpc.107.053306;
RA Prunet N., Morel P., Thierry A.-M., Eshed Y., Bowman J.L., Negrutiu I.,
RA Trehin C.;
RT "REBELOTE, SQUINT, and ULTRAPETALA1 function redundantly in the temporal
RT regulation of floral meristem termination in Arabidopsis thaliana.";
RL Plant Cell 20:901-919(2008).
RN [6]
RP INTERACTION WITH ENAP1; OBE1; SWA2; NOC2 AND NOC3, AND SUBCELLULAR
RP LOCATION.
RX PubMed=30338215; DOI=10.1002/2211-5463.12504;
RA de Bossoreille S., Morel P., Trehin C., Negrutiu I.;
RT "REBELOTE, a regulator of floral determinacy in Arabidopsis thaliana,
RT interacts with both nucleolar and nucleoplasmic proteins.";
RL FEBS Open Bio 8:1636-1648(2018).
CC -!- FUNCTION: Collaboratively with CYP40/SQN and ULT1, influences floral
CC meristem (FM) determinacy in an AGAMOUS and SUPERMAN-dependent manner,
CC thus contributing to the floral developmental homeostasis.
CC {ECO:0000269|PubMed:18441215}.
CC -!- SUBUNIT: Interacts with SWA2, NOC2 and NOC3 in both the nucleolus and
CC nucleoplasm (PubMed:30338215). Binds to ENAP1 and OBE1
CC (PubMed:30338215). {ECO:0000269|PubMed:30338215}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768,
CC ECO:0000269|PubMed:18441215}. Nucleus, nucleolus
CC {ECO:0000269|PubMed:30338215}. Nucleus, nucleoplasm
CC {ECO:0000269|PubMed:30338215}. Note=Observed in the nucleolus of root
CC cells in interphase and colocalizes with DNA in dividing root cells.
CC {ECO:0000269|PubMed:30338215}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in roots, shoots, leaves,
CC stems, inflorescences, flowers and siliques, with highest levels
CC dividing tissues. {ECO:0000269|PubMed:18441215}.
CC -!- DISRUPTION PHENOTYPE: Plants lacking both CRC and RBL exhibit strong
CC floral meristem (FM) indeterminacy with reiterations of extra floral
CC whorls in the center of the flower associated with reduced AGAMOUS and
CC SUPERMAN levels. {ECO:0000269|PubMed:18441215}.
CC -!- MISCELLANEOUS: 'Rebelote' means once again in French.
CC {ECO:0000305|PubMed:18441215}.
CC -!- SIMILARITY: Belongs to the NOC2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB75909.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL132975; CAB75909.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE79394.1; -; Genomic_DNA.
DR EMBL; AK226780; BAE98878.1; -; mRNA.
DR EMBL; AY085997; AAM63207.1; -; mRNA.
DR PIR; T47690; T47690.
DR RefSeq; NP_567023.1; NM_115408.4.
DR AlphaFoldDB; Q0WVH0; -.
DR STRING; 3702.AT3G55510.1; -.
DR PaxDb; Q0WVH0; -.
DR PRIDE; Q0WVH0; -.
DR ProteomicsDB; 175072; -.
DR EnsemblPlants; AT3G55510.1; AT3G55510.1; AT3G55510.
DR GeneID; 824716; -.
DR Gramene; AT3G55510.1; AT3G55510.1; AT3G55510.
DR Araport; AT3G55510; -.
DR TAIR; locus:2100006; AT3G55510.
DR eggNOG; KOG2256; Eukaryota.
DR HOGENOM; CLU_011272_2_1_1; -.
DR InParanoid; Q0WVH0; -.
DR OMA; HEQTTIE; -.
DR OrthoDB; 1194328at2759; -.
DR PhylomeDB; Q0WVH0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q0WVH0; baseline and differential.
DR GO; GO:0030690; C:Noc1p-Noc2p complex; IBA:GO_Central.
DR GO; GO:0030691; C:Noc2p-Noc3p complex; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0010582; P:floral meristem determinacy; IGI:TAIR.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; IBA:GO_Central.
DR InterPro; IPR005343; Noc2.
DR PANTHER; PTHR12687; PTHR12687; 2.
DR Pfam; PF03715; Noc2; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..594
FT /note="Protein REBELOTE"
FT /id="PRO_0000454495"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 35..58
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 8..15
FT /note="Nuclear localization signal 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT MOTIF 35..42
FT /note="Nuclear localization signal 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT MOTIF 512..519
FT /note="Nuclear localization signal 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 38..58
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 8
FT /note="A->T: In rbl-1; indeterminate flowers in crc-1
FT background."
FT /evidence="ECO:0000269|PubMed:18441215"
FT CONFLICT 380
FT /note="S -> A (in Ref. 4; AAM63207)"
FT /evidence="ECO:0000305"
FT CONFLICT 511
FT /note="R -> T (in Ref. 4; AAM63207)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 594 AA; 68367 MW; 26200EA2FADAF5DB CRC64;
MGKLGKKARK FAKKNLQSVE KRSRKLKPFI KKKFAKRNER HQAGDKQEKK VEQQPKKRCQ
EEEFQDIAID AVFGKDDDEV LRDGDSDSDG YLDELVNETD SDIMKCKVLS RSFLATCCDM
VDKEQYVPAL VRLLNWYRAA CQYGHEPSGI ARPNIYYDIE DSETFAKVII FVLQKADHTF
RSILGLSDSS TKEKILKLKN NPKWDSLKPL VKSFFRSTLH LVKQAGDLEI ISFTLTQLRV
SIVFLAAFPD LLKKLIKISV HLWVTGEETI SQQAFLILKD ISMVFNSECF DSCLINMYKA
FLHDCDIPKA NSEQRPFLRD SLVELCSQDV QKSYTKASVS ITQLAKLLKM ALATKNKEAV
EKIHSGEYIN CVDLWVNFIS ANVQDCDLQP LLYTIVQVIN GVAQLIIGPR YLLLRVKCIH
WLNHLSRTSG IFIPIASLVL DMLEYKTTND GEKQEQKLEA VSTVKLPKNW LKSQNFQEQC
IFSVIELLAV HFAQWSFHIS FPDLATIPVM RLKKFHERST MEGLKRVVKR FIEQVESNIE
FVQRKRDDVT FSPNDQQSAD TFMQLEKQNA NAPYTQYYQS IIDKALGTNK KKKK
