RBM12_MOUSE
ID RBM12_MOUSE Reviewed; 992 AA.
AC Q8R4X3; B6ICZ5; Q3TKT7; Q7TT14; Q8K373; Q8R302; Q9CS80;
DT 23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 03-NOV-2009, sequence version 3.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=RNA-binding protein 12;
DE AltName: Full=RNA-binding motif protein 12;
DE AltName: Full=SH3/WW domain anchor protein in the nucleus;
DE Short=SWAN;
GN Name=Rbm12;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Huang C.-H.;
RT "Identification of SWAN as a novel hnRNP-like adaptor protein with multiple
RT domains and broadly expressed in mammalian tissues.";
RL Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Blastocyst, Embryo, and Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Lung, and Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP STRUCTURE BY NMR OF 908-992.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the RNA binding domain of RNA-binding protein 12.";
RL Submitted (NOV-2005) to the PDB data bank.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH27810.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF345334; AAL83754.1; -; mRNA.
DR EMBL; AF393215; AAM73683.1; -; mRNA.
DR EMBL; AF393216; AAM73684.1; -; mRNA.
DR EMBL; AK017591; BAB30825.1; -; mRNA.
DR EMBL; AK029180; BAC26338.1; -; mRNA.
DR EMBL; AK035014; BAC28911.1; -; mRNA.
DR EMBL; AK080772; BAC38017.1; -; mRNA.
DR EMBL; AK166833; BAE39056.1; -; mRNA.
DR EMBL; AL833786; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC026891; AAH26891.2; -; mRNA.
DR EMBL; BC027810; AAH27810.2; ALT_INIT; mRNA.
DR EMBL; BC052473; AAH52473.1; -; mRNA.
DR CCDS; CCDS16961.1; -.
DR RefSeq; NP_083673.3; NM_029397.3.
DR RefSeq; NP_733486.2; NM_170598.2.
DR PDB; 2CQP; NMR; -; A=908-992.
DR PDBsum; 2CQP; -.
DR AlphaFoldDB; Q8R4X3; -.
DR SMR; Q8R4X3; -.
DR BioGRID; 217686; 6.
DR IntAct; Q8R4X3; 2.
DR STRING; 10090.ENSMUSP00000050461; -.
DR iPTMnet; Q8R4X3; -.
DR PhosphoSitePlus; Q8R4X3; -.
DR EPD; Q8R4X3; -.
DR jPOST; Q8R4X3; -.
DR MaxQB; Q8R4X3; -.
DR PaxDb; Q8R4X3; -.
DR PeptideAtlas; Q8R4X3; -.
DR PRIDE; Q8R4X3; -.
DR ProteomicsDB; 255036; -.
DR Antibodypedia; 35036; 156 antibodies from 22 providers.
DR DNASU; 75710; -.
DR Ensembl; ENSMUST00000059647; ENSMUSP00000050461; ENSMUSG00000089824.
DR Ensembl; ENSMUST00000109604; ENSMUSP00000105233; ENSMUSG00000089824.
DR GeneID; 75710; -.
DR KEGG; mmu:75710; -.
DR UCSC; uc008nmh.1; mouse.
DR CTD; 10137; -.
DR MGI; MGI:1922960; Rbm12.
DR VEuPathDB; HostDB:ENSMUSG00000089824; -.
DR eggNOG; KOG4307; Eukaryota.
DR GeneTree; ENSGT00940000160611; -.
DR HOGENOM; CLU_004368_1_0_1; -.
DR InParanoid; Q8R4X3; -.
DR OMA; PFSCSEV; -.
DR OrthoDB; 1067545at2759; -.
DR PhylomeDB; Q8R4X3; -.
DR TreeFam; TF331899; -.
DR BioGRID-ORCS; 75710; 5 hits in 72 CRISPR screens.
DR EvolutionaryTrace; Q8R4X3; -.
DR PRO; PR:Q8R4X3; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8R4X3; protein.
DR Bgee; ENSMUSG00000089824; Expressed in primitive streak and 242 other tissues.
DR ExpressionAtlas; Q8R4X3; baseline and differential.
DR Genevisible; Q8R4X3; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0043484; P:regulation of RNA splicing; IBA:GO_Central.
DR CDD; cd12745; RRM1_RBM12; 1.
DR CDD; cd12747; RRM2_RBM12; 1.
DR CDD; cd12512; RRM3_RBM12; 1.
DR CDD; cd12749; RRM4_RBM12; 1.
DR CDD; cd12751; RRM5_RBM12; 1.
DR Gene3D; 3.30.70.330; -; 5.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR034591; RBM12_RRM1.
DR InterPro; IPR034594; RBM12_RRM2.
DR InterPro; IPR034855; RBM12_RRM3.
DR InterPro; IPR034856; RBM12_RRM4.
DR InterPro; IPR034854; RBM12_RRM5.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 3.
DR SMART; SM00360; RRM; 4.
DR SUPFAM; SSF54928; SSF54928; 4.
DR PROSITE; PS50102; RRM; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Nucleus; Phosphoprotein; Reference proteome; Repeat;
KW RNA-binding.
FT CHAIN 1..992
FT /note="RNA-binding protein 12"
FT /id="PRO_0000081772"
FT DOMAIN 304..379
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 430..507
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 916..992
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 393..424
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 849..913
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 886..900
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 352
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NTZ6"
FT MOD_RES 375
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NTZ6"
FT MOD_RES 420
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NTZ6"
FT MOD_RES 422
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NTZ6"
FT MOD_RES 424
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NTZ6"
FT MOD_RES 525
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NTZ6"
FT CONFLICT 122
FT /note="I -> T (in Ref. 1; AAL83754/AAM73683/AAM73684 and 4;
FT AAH26891)"
FT /evidence="ECO:0000305"
FT CONFLICT 572
FT /note="S -> N (in Ref. 1; AAL83754/AAM73683/AAM73684 and 4;
FT AAH26891)"
FT /evidence="ECO:0000305"
FT CONFLICT 719
FT /note="G -> S (in Ref. 2; BAC28911)"
FT /evidence="ECO:0000305"
FT CONFLICT 729
FT /note="G -> S (in Ref. 2; BAB30825)"
FT /evidence="ECO:0000305"
FT CONFLICT 739
FT /note="G -> C (in Ref. 2; BAB30825)"
FT /evidence="ECO:0000305"
FT CONFLICT 756
FT /note="A -> AIPGPA (in Ref. 2; BAC26338)"
FT /evidence="ECO:0000305"
FT CONFLICT 756
FT /note="A -> AIPGPAIPGPT (in Ref. 1; AAL83754/AAM73683/
FT AAM73684 and 4; AAH26891/AAH27810)"
FT /evidence="ECO:0000305"
FT CONFLICT 808
FT /note="G -> GGG (in Ref. 1; AAL83754/AAM73683/AAM73684)"
FT /evidence="ECO:0000305"
FT CONFLICT 814
FT /note="V -> A (in Ref. 1; AAL83754/AAM73683/AAM73684)"
FT /evidence="ECO:0000305"
FT CONFLICT 817
FT /note="V -> G (in Ref. 1; AAL83754/AAM73683/AAM73684)"
FT /evidence="ECO:0000305"
FT CONFLICT 832
FT /note="E -> D (in Ref. 1; AAL83754/AAM73683/AAM73684)"
FT /evidence="ECO:0000305"
FT CONFLICT 839..840
FT /note="AP -> G (in Ref. 1; AAL83754/AAM73683/AAM73684)"
FT /evidence="ECO:0000305"
FT CONFLICT 897
FT /note="P -> L (in Ref. 1; AAL83754/AAM73683/AAM73684)"
FT /evidence="ECO:0000305"
FT STRAND 914..922
FT /evidence="ECO:0007829|PDB:2CQP"
FT HELIX 929..935
FT /evidence="ECO:0007829|PDB:2CQP"
FT TURN 936..938
FT /evidence="ECO:0007829|PDB:2CQP"
FT TURN 943..945
FT /evidence="ECO:0007829|PDB:2CQP"
FT STRAND 947..950
FT /evidence="ECO:0007829|PDB:2CQP"
FT STRAND 952..954
FT /evidence="ECO:0007829|PDB:2CQP"
FT STRAND 956..965
FT /evidence="ECO:0007829|PDB:2CQP"
FT HELIX 967..976
FT /evidence="ECO:0007829|PDB:2CQP"
FT TURN 977..979
FT /evidence="ECO:0007829|PDB:2CQP"
FT STRAND 988..992
FT /evidence="ECO:0007829|PDB:2CQP"
SQ SEQUENCE 992 AA; 102796 MW; 80FEBFDF17EE564F CRC64;
MAVVIRLQGL PIVAGTMDIR HFFSGLTIPD GGVHIVGGEL GEAFIVFATD EDARLGMMRT
GGTIKGSKVT LLLSSKTEMQ NMIELSRRRF ETANLDIPPA NASRSGPPPS SGMSSRVNLP
AIVPNFNNPS PSVVTATTSV HESNKNIQTF STASVGTAPP SMGTSFGSPT FSSTIPSTAS
PMNTVPPPPI PPIPAMPSLP PLPSIPPIPV PPPVPTLPPV PPVPPIPPVP SVPPMTTLPP
MSGMPPLNPP PVAPLPAGMN GSGAPIGLNN NMNPVFLGPL NPVNSIQMNS QSSVKSLPIN
PDDLYVSVHG MPFSAMENDV REFFHGLRVD AVHLLKDHVG RNNGNGLVKF LSPQDTFEAL
KRNRMLMIQR YVEVSPATER QWVAAGGHIT FKQSMGPSGQ AHPPPQTLPR SKSPSGQKRS
RSRSPHEAGF CVYLKGLPFE AENKHVIDFF KKLDIVEDSI YIAYGPNGKA TGEGFVEFRN
DADYKAALCR HKQYMGNRFI QVHPITKKGM LEKIDMIRKR LQNFSYDQRE LVLNPEGEVS
SAKVCAHITN IPFSITKMDV LQFLEGIPVD ESAVHVLVDN NGQGLGQALV QFKTEDDAHK
SEHLHRKKLN GREAFVHIVT LEDMREIEKN PPAQGKKGLK ISVPGNPAVP VIPSAGMPAA
GIPTAGIPGA GLPSAGMPGA GMPSSGMPGP GMPGPGIPGA GIPGPAMPGP AMPGPAMPGP
AMPGPAMPGP AMPGPAMPGP AMPGPAIPGP AIPGPAIPGA GIPSAGGEEH VFLTVGSKEA
NNGPPFNFPG NFGGPNAFGP PLPPPGLGGA FGDVRPVMPS VGNSGLPGLG LEVPGFGGAP
NNISGPSGFG GIPQNFGNGP GSLNAPPGFG SGPPGLGSVP GHLSGPPAFG PGPGPGPIHI
GGPPGFGASS GKPGPTIIKV QNMPFTVSID EILDFFYGYQ VIPGSVCLKY NEKGMPTGEA
MVAFESRDEA TAAVIDLNDR PIGSRKVKLV LG
