ID   RBM22_DANRE             Reviewed;         425 AA.
AC   Q6NZZ9;
DT   03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Pre-mRNA-splicing factor RBM22;
DE   AltName: Full=RNA-binding motif protein 22;
GN   Name=rbm22; ORFNames=zgc:77910;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RX   PubMed=15256591; DOI=10.1073/pnas.0403929101;
RA   Amsterdam A., Nissen R.M., Sun Z., Swindell E.C., Farrington S.,
RA   Hopkins N.;
RT   "Identification of 315 genes essential for early zebrafish development.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:12792-12797(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=20013661; DOI=10.4238/vol8-4gmr683;
RA   He F., Wang C.T., Gou L.T.;
RT   "RNA-binding motif protein RBM22 is required for normal development of
RT   zebrafish embryos.";
RL   Genet. Mol. Res. 8:1466-1473(2009).
CC   -!- FUNCTION: Required for pre-mRNA splicing as component of the activated
CC       spliceosome. Involved in the first step of pre-mRNA splicing. Binds
CC       directly to the internal stem-loop (ISL) domain of the U6 snRNA and to
CC       the pre-mRNA intron near the 5' splice site during the activation and
CC       catalytic phases of the spliceosome cycle (By similarity). Required for
CC       normal early embryogenesis (PubMed:20013661).
CC       {ECO:0000250|UniProtKB:Q9NW64, ECO:0000269|PubMed:20013661}.
CC   -!- SUBUNIT: Component of the pre-catalytic and catalytic spliceosome
CC       complexes. Component of the postcatalytic spliceosome P complex.
CC       {ECO:0000250|UniProtKB:Q9NW64}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9NW64}. Cytoplasm
CC       {ECO:0000250|UniProtKB:Q9NW64}. Note=Nearly exclusively nuclear. May be
CC       shuttling between the nucleus and the cytosol.
CC       {ECO:0000250|UniProtKB:Q9NW64}.
CC   -!- DEVELOPMENTAL STAGE: Maternally expressed. Expressed as early as the 1-
CC       cell stage and continued through the 8-cell and 32-cell stages and 15-
CC       hpf and 24-hpf stages. {ECO:0000269|PubMed:20013661}.
CC   -!- DOMAIN: The C-terminal RRM domain and the zinc finger motif are
CC       necessary for RNA-binding. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SLT11 family. {ECO:0000305}.
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DR   EMBL; AY648794; AAT68112.1; -; mRNA.
DR   EMBL; BC065892; AAH65892.1; -; mRNA.
DR   RefSeq; NP_998379.1; NM_213214.1.
DR   AlphaFoldDB; Q6NZZ9; -.
DR   SMR; Q6NZZ9; -.
DR   STRING; 7955.ENSDARP00000004055; -.
DR   PaxDb; Q6NZZ9; -.
DR   Ensembl; ENSDART00000003983; ENSDARP00000004055; ENSDARG00000010238.
DR   GeneID; 406495; -.
DR   KEGG; dre:406495; -.
DR   CTD; 55696; -.
DR   ZFIN; ZDB-GENE-040426-2298; rbm22.
DR   eggNOG; KOG0153; Eukaryota.
DR   GeneTree; ENSGT00390000002792; -.
DR   HOGENOM; CLU_027112_3_0_1; -.
DR   InParanoid; Q6NZZ9; -.
DR   OMA; QGQHYPY; -.
DR   OrthoDB; 1272857at2759; -.
DR   PhylomeDB; Q6NZZ9; -.
DR   TreeFam; TF314284; -.
DR   Reactome; R-DRE-72163; mRNA Splicing - Major Pathway.
DR   PRO; PR:Q6NZZ9; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 21.
DR   Bgee; ENSDARG00000010238; Expressed in gastrula and 28 other tissues.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IDA:ZFIN.
DR   GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR   GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; IBA:GO_Central.
DR   GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0036002; F:pre-mRNA binding; ISS:UniProtKB.
DR   GO; GO:0017070; F:U6 snRNA binding; ISS:UniProtKB.
DR   GO; GO:0043009; P:chordate embryonic development; IMP:ZFIN.
DR   GO; GO:0045292; P:mRNA cis splicing, via spliceosome; ISS:UniProtKB.
DR   GO; GO:0033120; P:positive regulation of RNA splicing; ISS:UniProtKB.
DR   Gene3D; 3.30.70.330; -; 1.
DR   InterPro; IPR039171; Cwc2/Slt11.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   InterPro; IPR000571; Znf_CCCH.
DR   InterPro; IPR036855; Znf_CCCH_sf.
DR   PANTHER; PTHR14089; PTHR14089; 1.
DR   Pfam; PF00076; RRM_1; 1.
DR   SMART; SM00360; RRM; 1.
DR   SMART; SM00356; ZnF_C3H1; 1.
DR   SUPFAM; SSF54928; SSF54928; 1.
DR   SUPFAM; SSF90229; SSF90229; 1.
DR   PROSITE; PS50102; RRM; 1.
DR   PROSITE; PS50103; ZF_C3H1; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Developmental protein; Metal-binding; mRNA processing;
KW   mRNA splicing; Nucleus; Reference proteome; RNA-binding; Spliceosome; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..425
FT                   /note="Pre-mRNA-splicing factor RBM22"
FT                   /id="PRO_0000250552"
FT   DOMAIN          232..305
FT                   /note="RRM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   ZN_FING         159..186
FT                   /note="C3H1-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT   REGION          304..331
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          384..425
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        384..402
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   425 AA;  47440 MW;  BD31C2638495E7F1 CRC64;
     MATSLGSNTY NRQNWEDSDF PILCQTCLGE NPYIRMTKEK FGKECKICAR PFTVFRWCPG
     VRMRFKKTEV CQTCSKMKNV CQTCLLDLEY GLPIQVRDTG LSVKDEVPRS DVNKEYYTQN
     MEREIANSDG TRPVGLLGKA PSSSDMLLKL ARTTPYYKRN RPHICSFWVK GECKRGEECP
     YRHEKPTDPD DPLADQNIKD RYYGINDPVA NKLLMRASTM PRLDVPDDKS ITTLYIGGLG
     ENVTDSELRN HFYQFGEIRT ITIVQRQQCA FIQFATRQAA ETAAEKSFNK LIINGRRLNV
     KWGRSQAARG KGEKDGVTES GIRLEPVPGL PGALPPPPVS DEDASTNYFN LAPTPSPAVM
     NLGLPPPPGV TMPPPPGFGP PMFHTMDPMA PPVPPPMALR PPGQVHYPSQ DPQRMGAHAS
     RHGGP