RBM24_DANRE
ID RBM24_DANRE Reviewed; 230 AA.
AC Q76LC6;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=RNA-binding protein 24 {ECO:0000305};
DE AltName: Full=RNA-binding motif protein 24 {ECO:0000250|UniProtKB:Q9BX46};
DE AltName: Full=RNA-binding protein SEB-4 {ECO:0000250|UniProtKB:Q6GQD3};
GN Name=rbm24;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Yamamoto S., Jin Y., Suzuki H., Maegawa S., Yasuda K., Inoue K.;
RT "The tissue-specific RNA binding protein SEB-4 binds UG dinucleotides
RT repeats in a SELEX system.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=22345307; DOI=10.1093/cvr/cvs095;
RA Poon K.L., Tan K.T., Wei Y.Y., Ng C.P., Colman A., Korzh V., Xu X.Q.;
RT "RNA-binding protein RBM24 is required for sarcomere assembly and heart
RT contractility.";
RL Cardiovasc. Res. 94:418-427(2012).
CC -!- FUNCTION: Multifunctional RNA-binding protein involved in the
CC regulation of pre-mRNA splicing, mRNA stability and mRNA translation
CC important for cell fate decision and differentiation. Plays a major
CC role in pre-mRNA alternative splicing regulation. Mediates
CC preferentially muscle-specific exon inclusion in numerous mRNAs
CC important for striated cardiac and skeletal muscle cell
CC differentiation. Binds to intronic splicing enhancer (ISE) composed of
CC stretches of GU-rich motifs localized in flanking intron of exon that
CC will be included by alternative splicing. Involved in embryonic stem
CC cell (ESC) transition to cardiac cell differentiation by promoting pre-
CC mRNA alternative splicing events of several pluripotency and/or
CC differentiation genes. Plays a role in the regulation of mRNA stability
CC and mRNA translation to which it is bound. Involved in myogenic
CC differentiation by regulating myog levels. Binds to a huge amount of
CC mRNAs (By similarity). Required for embryonic heart development,
CC sarcomer and M-band formation in striated muscles (PubMed:22345307).
CC {ECO:0000250|UniProtKB:D3Z4I3, ECO:0000250|UniProtKB:Q9BX46,
CC ECO:0000269|PubMed:22345307}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q6GQD3}. Cytoplasm
CC {ECO:0000250|UniProtKB:D3Z4I3}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the embryo from 2 to 72 hours post-
CC fertilization (hpf) (PubMed:22345307). Expressed in the embryonic heart
CC and somites at 14, 20 and 24 hpf (PubMed:22345307). Expressed in
CC myocardial progenitors from 18 to 24 hpf (PubMed:22345307). Expressed
CC in the otic vesicle at 14 hpf (PubMed:22345307).
CC {ECO:0000269|PubMed:22345307}.
CC -!- DOMAIN: The RRM domain is necessary for mRNA stability and mRNA
CC translation regulation. {ECO:0000250|UniProtKB:Q9BX46}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown of the protein leads to
CC death at 6 days post-fertilization (dpf) due to heart growth defects
CC and absence of blood circulation, despite the presence of a beating
CC heart (PubMed:22345307). Show reduced heart contractility from 30 hours
CC post-fertilization (hpf), onwards (PubMed:22345307). Display defects in
CC sarcomere organization from 33 hpf, onwards (PubMed:22345307). Show
CC altered expression of key genes necessary for sarcomere formation
CC (PubMed:22345307). {ECO:0000269|PubMed:22345307}.
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DR EMBL; AB080735; BAD12194.1; -; mRNA.
DR RefSeq; NP_998030.1; NM_212865.1.
DR AlphaFoldDB; Q76LC6; -.
DR SMR; Q76LC6; -.
DR STRING; 7955.ENSDARP00000100853; -.
DR PaxDb; Q76LC6; -.
DR Ensembl; ENSDART00000161826; ENSDARP00000135480; ENSDARG00000102995.
DR GeneID; 405801; -.
DR KEGG; dre:405801; -.
DR CTD; 405801; -.
DR ZFIN; ZDB-GENE-040628-1; rbm24a.
DR eggNOG; KOG0149; Eukaryota.
DR GeneTree; ENSGT00940000156245; -.
DR HOGENOM; CLU_065652_0_1_1; -.
DR InParanoid; Q76LC6; -.
DR OrthoDB; 1579773at2759; -.
DR PhylomeDB; Q76LC6; -.
DR TreeFam; TF314235; -.
DR PRO; PR:Q76LC6; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 19.
DR Bgee; ENSDARG00000102995; Expressed in somite and 44 other tissues.
DR ExpressionAtlas; Q76LC6; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0035925; F:mRNA 3'-UTR AU-rich region binding; ISS:UniProtKB.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; ISS:UniProtKB.
DR GO; GO:1990715; F:mRNA CDS binding; ISS:UniProtKB.
DR GO; GO:0097157; F:pre-mRNA intronic binding; ISS:UniProtKB.
DR GO; GO:1990825; F:sequence-specific mRNA binding; ISS:UniProtKB.
DR GO; GO:0061158; P:3'-UTR-mediated mRNA destabilization; ISS:UniProtKB.
DR GO; GO:0003228; P:atrial cardiac muscle tissue development; IMP:ZFIN.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; ISS:UniProtKB.
DR GO; GO:0072359; P:circulatory system development; IGI:ZFIN.
DR GO; GO:0048702; P:embryonic neurocranium morphogenesis; IMP:ZFIN.
DR GO; GO:0001947; P:heart looping; IMP:ZFIN.
DR GO; GO:0061157; P:mRNA destabilization; ISS:UniProtKB.
DR GO; GO:0006397; P:mRNA processing; IMP:ZFIN.
DR GO; GO:0048255; P:mRNA stabilization; ISS:UniProtKB.
DR GO; GO:2000766; P:negative regulation of cytoplasmic translation; ISS:UniProtKB.
DR GO; GO:1905870; P:positive regulation of 3'-UTR-mediated mRNA stabilization; ISS:UniProtKB.
DR GO; GO:0045663; P:positive regulation of myoblast differentiation; ISS:UniProtKB.
DR GO; GO:0010831; P:positive regulation of myotube differentiation; ISS:UniProtKB.
DR GO; GO:0060298; P:positive regulation of sarcomere organization; IMP:UniProtKB.
DR GO; GO:1902811; P:positive regulation of skeletal muscle fiber differentiation; ISS:UniProtKB.
DR GO; GO:2000738; P:positive regulation of stem cell differentiation; ISS:UniProtKB.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0043488; P:regulation of mRNA stability; ISS:UniProtKB.
DR GO; GO:0010830; P:regulation of myotube differentiation; ISS:UniProtKB.
DR GO; GO:0060297; P:regulation of sarcomere organization; IMP:ZFIN.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR GO; GO:0007519; P:skeletal muscle tissue development; IMP:ZFIN.
DR GO; GO:0001756; P:somitogenesis; IMP:ZFIN.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Differentiation; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome; RNA-binding; Translation regulation.
FT CHAIN 1..230
FT /note="RNA-binding protein 24"
FT /id="PRO_0000273372"
FT DOMAIN 11..88
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
SQ SEQUENCE 230 AA; 24601 MW; 9AE4463ED214E9EB CRC64;
MHTTQKDTTY TKIFVGGLPY HTTDSSLRKY FEVFGEIEEA VVITDRQTGK SRGYGFVTMA
DRSAADRACK DPNPIIDGRK ANVNLAYLGA KPRVMQPGFT FGVPQIHPAF IQRPYGIPTH
YVYPQAFMQP SVVIPHIQPT ATSATASSPY IDYTGAAYAQ YASAATAAAA AAYEQYPYAA
SPAATGYVAA AGYGYAMQQP LATAAPGSAA AAAAAFGQYQ PQQLQAERMQ
