RBM24_MOUSE
ID RBM24_MOUSE Reviewed; 236 AA.
AC D3Z4I3;
DT 21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT 20-APR-2010, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=RNA-binding protein 24 {ECO:0000305};
DE AltName: Full=RNA-binding motif protein 24 {ECO:0000250|UniProtKB:Q9BX46};
GN Name=Rbm24 {ECO:0000312|MGI:MGI:3610364};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP DEVELOPMENTAL STAGE.
RX PubMed=19658189; DOI=10.1002/stem.166;
RA Xu X.Q., Soo S.Y., Sun W., Zweigerdt R.;
RT "Global expression profile of highly enriched cardiomyocytes derived from
RT human embryonic stem cells.";
RL Stem Cells 27:2163-2174(2009).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=25313962; DOI=10.1016/j.devcel.2014.08.025;
RA Yang J., Hung L.H., Licht T., Kostin S., Looso M., Khrameeva E.,
RA Bindereif A., Schneider A., Braun T.;
RT "RBM24 is a major regulator of muscle-specific alternative splicing.";
RL Dev. Cell 31:87-99(2014).
RN [4]
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=25217815; DOI=10.1016/j.mod.2014.08.003;
RA Grifone R., Xie X., Bourgeois A., Saquet A., Duprez D., Shi D.L.;
RT "The RNA-binding protein Rbm24 is transiently expressed in myoblasts and is
RT required for myogenic differentiation during vertebrate development.";
RL Mech. Dev. 134:1-15(2014).
RN [5]
RP FUNCTION, AND INDUCTION.
RX PubMed=26844700; DOI=10.1038/cddis.2016.10;
RA Cardinali B., Cappella M., Provenzano C., Garcia-Manteiga J.M.,
RA Lazarevic D., Cittaro D., Martelli F., Falcone G.;
RT "MicroRNA-222 regulates muscle alternative splicing through Rbm24 during
RT differentiation of skeletal muscle cells.";
RL Cell Death Dis. 7:E2086-E2086(2016).
RN [6]
RP INDUCTION.
RX PubMed=26990106; DOI=10.1002/stem.2366;
RA Zhang T., Lin Y., Liu J., Zhang Z.G., Fu W., Guo L.Y., Pan L., Kong X.,
RA Zhang M.K., Lu Y.H., Huang Z.R., Xie Q., Li W.H., Xu X.Q.;
RT "Rbm24 regulates alternative splicing switch in embryonic stem cell cardiac
RT lineage differentiation.";
RL Stem Cells 34:1776-1789(2016).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=29358667; DOI=10.1038/s41418-017-0029-8;
RA Zhang M., Zhang Y., Xu E., Mohibi S., de Anda D.M., Jiang Y., Zhang J.,
RA Chen X.;
RT "Rbm24, a target of p53, is necessary for proper expression of p53 and
RT heart development.";
RL Cell Death Differ. 25:1118-1130(2018).
CC -!- FUNCTION: Multifunctional RNA-binding protein involved in the
CC regulation of pre-mRNA splicing, mRNA stability and mRNA translation
CC important for cell fate decision and differentiation (PubMed:25313962,
CC PubMed:26844700). Plays a major role in pre-mRNA alternative splicing
CC regulation (PubMed:25313962, PubMed:26844700). Mediates preferentially
CC muscle-specific exon inclusion in numerous mRNAs important for striated
CC cardiac and skeletal muscle cell differentiation (PubMed:25313962,
CC PubMed:26844700). Binds to intronic splicing enhancer (ISE) composed of
CC stretches of GU-rich motifs localized in flanking intron of exon that
CC will be included by alternative splicing (PubMed:25313962). Involved in
CC embryonic stem cell (ESC) transition to cardiac cell differentiation by
CC promoting pre-mRNA alternative splicing events of several pluripotency
CC and/or differentiation genes. Plays a role in the regulation of mRNA
CC stability. Binds to 3'-untranslated region (UTR) AU-rich elements in
CC target transcripts, such as CDKN1A and MYOG, leading to maintain their
CC stabilities. Involved in myogenic differentiation by regulating MYOG
CC levels. Binds to multiple regions in the mRNA 3'-UTR of TP63, hence
CC inducing its destabilization. Promotes also the destabilization of the
CC CHRM2 mRNA via its binding to a region in the coding sequence. Plays a
CC role in the regulation of mRNA translation. Mediates repression of
CC p53/TP53 mRNA translation through its binding to U-rich element in the
CC 3'-UTR, hence preventing EIF4E from binding to p53/TP53 mRNA and
CC translation initiation. Binds to a huge amount of mRNAs (By
CC similarity). Required for embryonic heart development, sarcomer and M-
CC band formation in striated muscles (PubMed:25313962, PubMed:29358667).
CC Together with RBM20, promotes the expression of short isoforms of
CC PDLIM5/ENH in cardiomyocytes (By similarity).
CC {ECO:0000250|UniProtKB:M0R7T6, ECO:0000250|UniProtKB:Q9BX46,
CC ECO:0000269|PubMed:25313962, ECO:0000269|PubMed:26844700,
CC ECO:0000269|PubMed:29358667}.
CC -!- SUBUNIT: Interacts with EIF4E; this interaction prevents EIF4E from
CC binding to p53/TP53 mRNA and inhibits the assembly of translation
CC initiation complex. {ECO:0000250|UniProtKB:Q9BX46}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q6GQD3}. Cytoplasm
CC {ECO:0000269|PubMed:25217815}.
CC -!- TISSUE SPECIFICITY: Expressed strongly in heart and skeletal muscles
CC (PubMed:25313962). Weakly expressed in intestine, aorta, liver, lung,
CC kidney, uterus and bladder (PubMed:25313962).
CC {ECO:0000269|PubMed:25313962}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryonic heart at 11.5 and 13.5 dpc
CC (PubMed:25313962). Expressed in MyoD-expressing myoblasts at 11.5 dpc
CC (at protein level) (PubMed:25217815). Expressed in the early cardiac
CC mesoderm at 7 dpc (PubMed:19658189). Expressed in the cardiac crescent
CC at 8 dpc (PubMed:19658189). Expressed in the developing heart and
CC somites from 9.5 to 12.5 dpc (PubMed:19658189, PubMed:25313962,
CC PubMed:25217815). Expressed in the lens, otic vesicle, heart and weakly
CC in the mesodermal core of the first and second branchial arches at 10.5
CC dpc (PubMed:25217815). Expressed in all appendicular muscle masses at
CC the forelimb and hindlimb levels and in developing head muscles at 11.5
CC dpc (PubMed:25217815). Expressed in appendicular muscle masses,
CC diaphragm muscle and developing head muscles at 12.5 dpc
CC (PubMed:25217815). {ECO:0000269|PubMed:19658189,
CC ECO:0000269|PubMed:25217815, ECO:0000269|PubMed:25313962}.
CC -!- INDUCTION: Up-regulated during embryonic stem cell (ESC)
CC differentiation into cardiomyocytes (PubMed:26990106). Down-regulated
CC by microRNA-222 (miR-222) in skeletal muscle cells, leading in
CC inhibition of muscle-specific pre-mRNA alternative splicing events and
CC myoblast fusion (PubMed:26844700). {ECO:0000269|PubMed:26844700,
CC ECO:0000269|PubMed:26990106}.
CC -!- DOMAIN: The RRM domain is necessary for mRNA stability and mRNA
CC translation regulation. {ECO:0000250|UniProtKB:Q9BX46}.
CC -!- DISRUPTION PHENOTYPE: Mice die in utero between embryonic days 12.5 and
CC 14.5 (PubMed:25313962, PubMed:29358667). Show multiple cardiac
CC malformations, including defective endocardial cushion morphogenesis,
CC ventricular septum defects, reduced trabeculation and compaction and
CC dilated atria (PubMed:25313962, PubMed:29358667). Display loss of
CC sarcomeres in cardiomyocytes (PubMed:25313962). Show aberrant pre-mRNA
CC alternative splicing of key genes necessary for sarcomere formation and
CC cardiogenesis (PubMed:25313962). {ECO:0000269|PubMed:25313962,
CC ECO:0000269|PubMed:29358667}.
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DR EMBL; AC124411; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS36651.1; -.
DR RefSeq; NP_001074894.1; NM_001081425.1.
DR AlphaFoldDB; D3Z4I3; -.
DR SMR; D3Z4I3; -.
DR STRING; 10090.ENSMUSP00000043120; -.
DR PhosphoSitePlus; D3Z4I3; -.
DR MaxQB; D3Z4I3; -.
DR PaxDb; D3Z4I3; -.
DR PeptideAtlas; D3Z4I3; -.
DR PRIDE; D3Z4I3; -.
DR ProteomicsDB; 255123; -.
DR Antibodypedia; 44354; 91 antibodies from 20 providers.
DR Ensembl; ENSMUST00000037923; ENSMUSP00000043120; ENSMUSG00000038132.
DR GeneID; 666794; -.
DR KEGG; mmu:666794; -.
DR UCSC; uc007qhe.1; mouse.
DR CTD; 221662; -.
DR MGI; MGI:3610364; Rbm24.
DR VEuPathDB; HostDB:ENSMUSG00000038132; -.
DR eggNOG; KOG0149; Eukaryota.
DR eggNOG; KOG4205; Eukaryota.
DR GeneTree; ENSGT00940000156245; -.
DR HOGENOM; CLU_065652_0_1_1; -.
DR InParanoid; D3Z4I3; -.
DR OMA; IPAHYMY; -.
DR OrthoDB; 1579773at2759; -.
DR PhylomeDB; D3Z4I3; -.
DR TreeFam; TF314235; -.
DR BioGRID-ORCS; 666794; 5 hits in 76 CRISPR screens.
DR ChiTaRS; Rbm24; mouse.
DR PRO; PR:D3Z4I3; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; D3Z4I3; protein.
DR Bgee; ENSMUSG00000038132; Expressed in interventricular septum and 187 other tissues.
DR Genevisible; D3Z4I3; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0035925; F:mRNA 3'-UTR AU-rich region binding; ISS:UniProtKB.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IDA:UniProtKB.
DR GO; GO:1990715; F:mRNA CDS binding; ISS:UniProtKB.
DR GO; GO:0097157; F:pre-mRNA intronic binding; IDA:UniProtKB.
DR GO; GO:1990825; F:sequence-specific mRNA binding; ISS:UniProtKB.
DR GO; GO:0061158; P:3'-UTR-mediated mRNA destabilization; ISS:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; ISS:UniProtKB.
DR GO; GO:0003197; P:endocardial cushion development; IMP:UniProtKB.
DR GO; GO:0061157; P:mRNA destabilization; ISS:UniProtKB.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0048255; P:mRNA stabilization; ISS:UniProtKB.
DR GO; GO:2000766; P:negative regulation of cytoplasmic translation; ISS:UniProtKB.
DR GO; GO:1905870; P:positive regulation of 3'-UTR-mediated mRNA stabilization; ISS:UniProtKB.
DR GO; GO:0045663; P:positive regulation of myoblast differentiation; ISS:UniProtKB.
DR GO; GO:0010831; P:positive regulation of myotube differentiation; ISS:UniProtKB.
DR GO; GO:1902811; P:positive regulation of skeletal muscle fiber differentiation; IMP:UniProtKB.
DR GO; GO:2000738; P:positive regulation of stem cell differentiation; ISS:UniProtKB.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IMP:UniProtKB.
DR GO; GO:0043488; P:regulation of mRNA stability; IMP:UniProtKB.
DR GO; GO:0010830; P:regulation of myotube differentiation; IMP:UniProtKB.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Differentiation; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome; RNA-binding; Translation regulation.
FT CHAIN 1..236
FT /note="RNA-binding protein 24"
FT /id="PRO_0000416117"
FT DOMAIN 11..88
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 175..199
FT /note="Necessary for interaction with EIF4E"
FT /evidence="ECO:0000250|UniProtKB:Q9BX46"
SQ SEQUENCE 236 AA; 24822 MW; 770ECF253A088E25 CRC64;
MHTTQKDTTY TKIFVGGLPY HTTDASLRKY FEVFGDIEEA VVITDRQTGK SRGYGFVTMA
DRAAAERACK DPNPIIDGRK ANVNLAYLGA KPRIMQPGFA FGVQQLHPAL IQRPFGIPAH
YVYPQAFVQP GVVIPHVQPT AAAASTTPYI DYTGAAYAQY SAAAAAAAAA AAYDQYPYAA
SPAAAGYVTT GGYSYAVQQP ITAAAPGTAA AAAAAAAAAA AFGQYQPQQL QTDRMQ
