RBM3_CAPHI
ID RBM3_CAPHI Reviewed; 160 AA.
AC W8E7I1;
DT 11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT 11-NOV-2015, sequence version 2.
DT 03-AUG-2022, entry version 38.
DE RecName: Full=RNA-binding protein 3 {ECO:0000312|EMBL:AHJ80940.1};
DE AltName: Full=RNA-binding motif protein 3 {ECO:0000303|PubMed:25544695};
GN Name=RBM3 {ECO:0000303|PubMed:25544695};
OS Capra hircus (Goat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Capra.
OX NCBI_TaxID=9925;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INDUCTION (ISOFORM 2), AND
RP PHYLOGENETIC ANALYSIS.
RC TISSUE=Blood {ECO:0000303|PubMed:25544695};
RX PubMed=25544695; DOI=10.1016/j.rvsc.2014.11.016;
RA Zargar R., Urwat U., Malik F., Shah R.A., Bhat M.H., Naykoo N.A., Khan F.,
RA Khan H.M., Ahmed S.M., Vijh R.K., Ganai N.A.;
RT "Molecular characterization of RNA binding motif protein 3 (RBM3) gene from
RT Pashmina goat.";
RL Res. Vet. Sci. 98:51-58(2015).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23263233; DOI=10.1038/nbt.2478;
RA Dong Y., Xie M., Jiang Y., Xiao N., Du X., Zhang W., Tosser-Klopp G.,
RA Wang J., Yang S., Liang J., Chen W., Chen J., Zeng P., Hou Y., Bian C.,
RA Pan S., Li Y., Liu X., Wang W., Servin B., Sayre B., Zhu B., Sweeney D.,
RA Moore R., Nie W., Shen Y., Zhao R., Zhang G., Li J., Faraut T., Womack J.,
RA Zhang Y., Kijas J., Cockett N., Xu X., Zhao S., Wang J., Wang W.;
RT "Sequencing and automated whole-genome optical mapping of the genome of a
RT domestic goat (Capra hircus).";
RL Nat. Biotechnol. 31:135-141(2013).
CC -!- FUNCTION: Cold-inducible mRNA binding protein that enhances global
CC protein synthesis at both physiological and mild hypothermic
CC temperatures. Reduces the relative abundance of microRNAs, when
CC overexpressed. Enhances phosphorylation of translation initiation
CC factors and active polysome formation. {ECO:0000250|UniProtKB:O89086}.
CC -!- SUBUNIT: Interacts with RPL4. Associates with the 60S ribosomal
CC subunits. {ECO:0000250|UniProtKB:Q925G0}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q925G0}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q925G0}. Cell projection, dendrite
CC {ECO:0000250|UniProtKB:Q925G0}. Note=Localizes in mRNA granules in
CC dentrites. {ECO:0000250|UniProtKB:Q925G0}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=W8E7I1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=W8E7I1-2; Sequence=VSP_057972, VSP_057973;
CC -!- INDUCTION: [Isoform 2]: Up-regulated significantly higher under
CC moderate hypothermic stress conditions (25-30 degrees Celsius) than
CC under deep hypothermia (10-15 degrees Celsius).
CC {ECO:0000269|PubMed:25544695}.
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DR EMBL; KF933377; AHJ80940.1; -; mRNA.
DR EMBL; AJPT01242208; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_005700842.1; XM_005700785.3. [W8E7I1-1]
DR RefSeq; XP_005700843.1; XM_005700786.3. [W8E7I1-1]
DR RefSeq; XP_013831916.1; XM_013976462.2. [W8E7I1-1]
DR AlphaFoldDB; W8E7I1; -.
DR SMR; W8E7I1; -.
DR STRING; 9925.ENSCHIP00000004879; -.
DR Ensembl; ENSCHIT00000012418; ENSCHIP00000004879; ENSCHIG00000009007. [W8E7I1-1]
DR GeneID; 102176209; -.
DR KEGG; chx:102176209; -.
DR CTD; 5935; -.
DR GeneTree; ENSGT00940000153524; -.
DR OMA; WHTTDDA; -.
DR OrthoDB; 1579773at2759; -.
DR Proteomes; UP000291000; Unassembled WGS sequence.
DR Bgee; ENSCHIG00000009007; Expressed in ovary and 18 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0030425; C:dendrite; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0043023; F:ribosomal large subunit binding; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0070417; P:cellular response to cold; IEP:UniProtKB.
DR GO; GO:0009631; P:cold acclimation; IEP:UniProtKB.
DR GO; GO:0045727; P:positive regulation of translation; ISS:UniProtKB.
DR GO; GO:0006417; P:regulation of translation; ISS:UniProtKB.
DR GO; GO:0009409; P:response to cold; ISS:UniProtKB.
DR CDD; cd12449; RRM_CIRBP_RBM3; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR034278; RBM3/CIRBP_RRM.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell projection; Cytoplasm; Methylation; Nucleus;
KW Phosphoprotein; Reference proteome; RNA-binding; Stress response.
FT CHAIN 1..160
FT /note="RNA-binding protein 3"
FT /id="PRO_0000434651"
FT DOMAIN 6..84
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 81..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 135..160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 47
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0000250|UniProtKB:P98179"
FT MOD_RES 105
FT /note="Asymmetric dimethylarginine; alternate"
FT /evidence="ECO:0000250|UniProtKB:O89086"
FT MOD_RES 105
FT /note="Dimethylated arginine; alternate"
FT /evidence="ECO:0000250|UniProtKB:P98179"
FT MOD_RES 105
FT /note="Omega-N-methylarginine; alternate"
FT /evidence="ECO:0000250|UniProtKB:P98179"
FT MOD_RES 120
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0000250|UniProtKB:P98179"
FT MOD_RES 134
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0000250|UniProtKB:P98179"
FT MOD_RES 150
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P98179"
FT MOD_RES 158
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:P98179"
FT VAR_SEQ 71..90
FT /note="SLDGRQIRVDHAGKSARGSR -> GVEGSGRQSRAAWEATTGPA (in
FT isoform 2)"
FT /id="VSP_057972"
FT VAR_SEQ 91..160
FT /note="Missing (in isoform 2)"
FT /id="VSP_057973"
SQ SEQUENCE 160 AA; 17526 MW; F9A754C4E0B13DB9 CRC64;
MSSEEGKLFV GGLNFNTDER ALEDHFSSFG PISEVVVVKD RETQRSRGFG FITFTNPEHA
SNAMRAMNGE SLDGRQIRVD HAGKSARGSR GGAFGSYERG RGYPRGGGDQ GYGSGRYDNR
PGAYGFGYGY GYGRSRDYGG RSQGGYDRYS GGNYRDNYDN
