RBM45_HUMAN
ID RBM45_HUMAN Reviewed; 476 AA.
AC Q8IUH3; Q6NYL0; Q8NFC9;
DT 15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=RNA-binding protein 45;
DE AltName: Full=Developmentally-regulated RNA-binding protein 1;
DE Short=RB-1;
DE AltName: Full=RNA-binding motif protein 45;
GN Name=RBM45; Synonyms=DRB1 {ECO:0000312|EMBL:AAH66549.1}, DRBP1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1] {ECO:0000305, ECO:0000312|EMBL:BAC16207.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, AND SUBCELLULAR LOCATION.
RC TISSUE=Brain {ECO:0000312|EMBL:BAC16207.1};
RX PubMed=12220514; DOI=10.1016/s0006-291x(02)02132-0;
RA Tamada H., Sakashita E., Shimazaki K., Ueno E., Hamamoto T., Kagawa Y.,
RA Endo H.;
RT "cDNA cloning and characterization of Drb1, a new member of RRM-type neural
RT RNA-binding protein.";
RL Biochem. Biophys. Res. Commun. 297:96-104(2002).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAM88417.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC TISSUE=Heart {ECO:0000312|EMBL:AAM88417.1};
RA Chen X.G., Li Y.;
RL Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAH66549.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Pancreas {ECO:0000312|EMBL:AAH66549.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-464, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [5]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-34, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- FUNCTION: RNA-binding protein with binding specificity for poly(C). May
CC play an important role in neural development.
CC {ECO:0000250|UniProtKB:Q8CFD1, ECO:0000269|PubMed:12220514}.
CC -!- INTERACTION:
CC Q8IUH3; Q9NW38: FANCL; NbExp=3; IntAct=EBI-2512147, EBI-2339898;
CC Q8IUH3; Q9Y316: MEMO1; NbExp=3; IntAct=EBI-2512147, EBI-1104564;
CC Q8IUH3; Q13077: TRAF1; NbExp=3; IntAct=EBI-2512147, EBI-359224;
CC Q8IUH3; Q99757: TXN2; NbExp=3; IntAct=EBI-2512147, EBI-2932492;
CC Q8IUH3-3; Q15699: ALX1; NbExp=3; IntAct=EBI-10964453, EBI-750671;
CC Q8IUH3-3; Q8WXH2: JPH3; NbExp=3; IntAct=EBI-10964453, EBI-1055254;
CC Q8IUH3-3; Q96HA8: NTAQ1; NbExp=3; IntAct=EBI-10964453, EBI-741158;
CC Q8IUH3-3; Q8IUH3-3: RBM45; NbExp=3; IntAct=EBI-10964453, EBI-10964453;
CC Q8IUH3-3; O14787-2: TNPO2; NbExp=3; IntAct=EBI-10964453, EBI-12076664;
CC Q8IUH3-3; Q13077: TRAF1; NbExp=3; IntAct=EBI-10964453, EBI-359224;
CC Q8IUH3-3; Q99757: TXN2; NbExp=3; IntAct=EBI-10964453, EBI-2932492;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12220514}. Nucleus
CC {ECO:0000269|PubMed:12220514}. Note=Predominantly cytoplasmic. May
CC shuttle between cytoplasm and nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1 {ECO:0000269|PubMed:12220514};
CC IsoId=Q8IUH3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8IUH3-2; Sequence=VSP_021005, VSP_051661, VSP_051662;
CC Name=3;
CC IsoId=Q8IUH3-3; Sequence=VSP_021005;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to an intron retention.
CC {ECO:0000305}.
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DR EMBL; AB036991; BAC16207.1; -; mRNA.
DR EMBL; AF526533; AAM88417.1; -; mRNA.
DR EMBL; BC066549; AAH66549.1; -; mRNA.
DR CCDS; CCDS33335.1; -. [Q8IUH3-3]
DR RefSeq; NP_694453.2; NM_152945.3. [Q8IUH3-3]
DR RefSeq; XP_005246344.1; XM_005246287.4.
DR RefSeq; XP_016858811.1; XM_017003322.1.
DR PDB; 7CSX; X-ray; 2.50 A; A=23-202.
DR PDB; 7CSZ; X-ray; 1.80 A; A=23-202.
DR PDBsum; 7CSX; -.
DR PDBsum; 7CSZ; -.
DR AlphaFoldDB; Q8IUH3; -.
DR SMR; Q8IUH3; -.
DR BioGRID; 126210; 212.
DR IntAct; Q8IUH3; 55.
DR MINT; Q8IUH3; -.
DR STRING; 9606.ENSP00000286070; -.
DR iPTMnet; Q8IUH3; -.
DR PhosphoSitePlus; Q8IUH3; -.
DR BioMuta; RBM45; -.
DR DMDM; 59797947; -.
DR EPD; Q8IUH3; -.
DR jPOST; Q8IUH3; -.
DR MassIVE; Q8IUH3; -.
DR MaxQB; Q8IUH3; -.
DR PaxDb; Q8IUH3; -.
DR PeptideAtlas; Q8IUH3; -.
DR PRIDE; Q8IUH3; -.
DR ProteomicsDB; 70566; -. [Q8IUH3-1]
DR ProteomicsDB; 70567; -. [Q8IUH3-2]
DR ProteomicsDB; 70568; -. [Q8IUH3-3]
DR Antibodypedia; 19584; 138 antibodies from 23 providers.
DR DNASU; 129831; -.
DR Ensembl; ENST00000286070.10; ENSP00000286070.5; ENSG00000155636.15. [Q8IUH3-3]
DR Ensembl; ENST00000616198.4; ENSP00000482083.1; ENSG00000155636.15. [Q8IUH3-1]
DR GeneID; 129831; -.
DR KEGG; hsa:129831; -.
DR MANE-Select; ENST00000286070.10; ENSP00000286070.5; NM_152945.4; NP_694453.2. [Q8IUH3-3]
DR UCSC; uc002ulv.4; human. [Q8IUH3-1]
DR CTD; 129831; -.
DR DisGeNET; 129831; -.
DR GeneCards; RBM45; -.
DR HGNC; HGNC:24468; RBM45.
DR HPA; ENSG00000155636; Low tissue specificity.
DR MIM; 608888; gene.
DR neXtProt; NX_Q8IUH3; -.
DR OpenTargets; ENSG00000155636; -.
DR PharmGKB; PA162400809; -.
DR VEuPathDB; HostDB:ENSG00000155636; -.
DR eggNOG; ENOG502QTJ8; Eukaryota.
DR GeneTree; ENSGT00680000100059; -.
DR HOGENOM; CLU_035274_1_0_1; -.
DR InParanoid; Q8IUH3; -.
DR OMA; ILFHPHP; -.
DR OrthoDB; 673895at2759; -.
DR PhylomeDB; Q8IUH3; -.
DR TreeFam; TF324216; -.
DR PathwayCommons; Q8IUH3; -.
DR SignaLink; Q8IUH3; -.
DR BioGRID-ORCS; 129831; 13 hits in 1032 CRISPR screens.
DR ChiTaRS; RBM45; human.
DR GenomeRNAi; 129831; -.
DR Pharos; Q8IUH3; Tbio.
DR PRO; PR:Q8IUH3; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q8IUH3; protein.
DR Bgee; ENSG00000155636; Expressed in mucosa of transverse colon and 162 other tissues.
DR ExpressionAtlas; Q8IUH3; baseline and differential.
DR Genevisible; Q8IUH3; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0003723; F:RNA binding; IDA:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007399; P:nervous system development; ISS:UniProtKB.
DR CDD; cd12366; RRM1_RBM45; 1.
DR CDD; cd12367; RRM2_RBM45; 1.
DR CDD; cd12368; RRM3_RBM45; 1.
DR CDD; cd12369; RRM4_RBM45; 1.
DR Gene3D; 3.30.70.330; -; 3.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR033101; RBM45.
DR InterPro; IPR034203; RBM45_RRM1.
DR InterPro; IPR034206; RBM45_RRM2.
DR InterPro; IPR034207; RBM45_RRM3.
DR InterPro; IPR034208; RBM45_RRM4.
DR InterPro; IPR000504; RRM_dom.
DR PANTHER; PTHR10352:SF18; PTHR10352:SF18; 1.
DR Pfam; PF00076; RRM_1; 4.
DR SMART; SM00360; RRM; 4.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cytoplasm; Developmental protein;
KW Differentiation; Isopeptide bond; Neurogenesis; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; RNA-binding; Ubl conjugation.
FT CHAIN 1..476
FT /note="RNA-binding protein 45"
FT /id="PRO_0000081572"
FT DOMAIN 26..106
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 121..195
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 392..464
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 199
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8BHN5"
FT MOD_RES 464
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT CROSSLNK 34
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT VAR_SEQ 225..226
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.2"
FT /id="VSP_021005"
FT VAR_SEQ 330
FT /note="D -> E (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_051661"
FT VAR_SEQ 331..476
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_051662"
FT STRAND 27..31
FT /evidence="ECO:0007829|PDB:7CSZ"
FT HELIX 38..45
FT /evidence="ECO:0007829|PDB:7CSZ"
FT HELIX 46..48
FT /evidence="ECO:0007829|PDB:7CSZ"
FT STRAND 51..58
FT /evidence="ECO:0007829|PDB:7CSZ"
FT TURN 60..62
FT /evidence="ECO:0007829|PDB:7CSZ"
FT STRAND 65..75
FT /evidence="ECO:0007829|PDB:7CSZ"
FT HELIX 76..86
FT /evidence="ECO:0007829|PDB:7CSZ"
FT HELIX 93..95
FT /evidence="ECO:0007829|PDB:7CSX"
FT STRAND 100..103
FT /evidence="ECO:0007829|PDB:7CSZ"
FT STRAND 107..109
FT /evidence="ECO:0007829|PDB:7CSZ"
FT HELIX 117..119
FT /evidence="ECO:0007829|PDB:7CSZ"
FT STRAND 121..126
FT /evidence="ECO:0007829|PDB:7CSZ"
FT HELIX 133..140
FT /evidence="ECO:0007829|PDB:7CSZ"
FT HELIX 141..143
FT /evidence="ECO:0007829|PDB:7CSZ"
FT STRAND 146..153
FT /evidence="ECO:0007829|PDB:7CSZ"
FT TURN 155..157
FT /evidence="ECO:0007829|PDB:7CSZ"
FT STRAND 160..170
FT /evidence="ECO:0007829|PDB:7CSZ"
FT HELIX 171..180
FT /evidence="ECO:0007829|PDB:7CSZ"
FT HELIX 183..185
FT /evidence="ECO:0007829|PDB:7CSZ"
SQ SEQUENCE 476 AA; 53502 MW; 06C7806D9079D391 CRC64;
MDEAGSSASG GGFRPGVDSL DEPPNSRIFL VISKYTPESV LRERFSPFGD IQDIWVVRDK
HTKESKGIAF VKFARSSQAC RAMEEMHGQC LGPNDTKPIK VFIAQSRSSG SHRDVEDEEL
TRIFVMIPKS YTEEDLREKF KVYGDIEYCS IIKNKVTGES KGLGYVRYLK PSQAAQAIEN
CDRSFRAILA EPKNKASESS EQDYYSNMRQ EALGHEPRVN MFPFVGEQQS EFSSFDKNDS
RGQEAISKRL SVVSRVPFTE EQLFSIFDIV PGLEYCEVQR DPYSNYGHGV VQYFNVASAI
YAKYKLHGFQ YPPGNRIGVS FIDDGSNATD LLRKMATQMV AAQLASMVWN NPSQQQFMQF
GGSSGSQLPQ IQTDVVLPSC KKKAPAETPV KERLFIVFNP HPLPLDVLED IFCRFGNLIE
VYLVSGKNVG YAKYADRISA NDAIATLHGK ILNGVRLKVM LADSPREESN KRQRTY
