RBM46_HUMAN
ID RBM46_HUMAN Reviewed; 533 AA.
AC Q8TBY0; B3KWU8; B4DZ27;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Probable RNA-binding protein 46;
DE AltName: Full=Cancer/testis antigen 68;
DE Short=CT68;
DE AltName: Full=RNA-binding motif protein 46;
GN Name=RBM46;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- INTERACTION:
CC Q8TBY0; P28329-3: CHAT; NbExp=3; IntAct=EBI-12068216, EBI-25837549;
CC Q8TBY0; P22607: FGFR3; NbExp=3; IntAct=EBI-12068216, EBI-348399;
CC Q8TBY0; O75593: FOXH1; NbExp=3; IntAct=EBI-12068216, EBI-1759806;
CC Q8TBY0; Q9H8Y8: GORASP2; NbExp=3; IntAct=EBI-12068216, EBI-739467;
CC Q8TBY0; P06396: GSN; NbExp=3; IntAct=EBI-12068216, EBI-351506;
CC Q8TBY0; P01112: HRAS; NbExp=3; IntAct=EBI-12068216, EBI-350145;
CC Q8TBY0; Q6ZRY4: RBPMS2; NbExp=3; IntAct=EBI-12068216, EBI-11987469;
CC Q8TBY0; Q13148: TARDBP; NbExp=3; IntAct=EBI-12068216, EBI-372899;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8TBY0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8TBY0-2; Sequence=VSP_055251;
CC Name=3;
CC IsoId=Q8TBY0-3; Sequence=VSP_055252;
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DR EMBL; AK125877; BAG54260.1; -; mRNA.
DR EMBL; AK302720; BAG63939.1; -; mRNA.
DR EMBL; AC009567; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471056; EAX04902.1; -; Genomic_DNA.
DR EMBL; CH471056; EAX04903.1; -; Genomic_DNA.
DR EMBL; BC028588; AAH28588.1; -; mRNA.
DR CCDS; CCDS3790.1; -. [Q8TBY0-1]
DR CCDS; CCDS64085.1; -. [Q8TBY0-3]
DR CCDS; CCDS64086.1; -. [Q8TBY0-2]
DR RefSeq; NP_001264100.1; NM_001277171.1. [Q8TBY0-3]
DR RefSeq; NP_001264102.1; NM_001277173.1. [Q8TBY0-2]
DR RefSeq; NP_659416.1; NM_144979.4. [Q8TBY0-1]
DR RefSeq; XP_006714182.1; XM_006714119.2. [Q8TBY0-3]
DR RefSeq; XP_006714183.1; XM_006714120.1. [Q8TBY0-2]
DR RefSeq; XP_011529994.1; XM_011531692.1. [Q8TBY0-1]
DR RefSeq; XP_011529995.1; XM_011531693.1. [Q8TBY0-1]
DR RefSeq; XP_011529996.1; XM_011531694.1. [Q8TBY0-1]
DR RefSeq; XP_011529997.1; XM_011531695.2. [Q8TBY0-1]
DR RefSeq; XP_011529998.1; XM_011531696.1. [Q8TBY0-1]
DR AlphaFoldDB; Q8TBY0; -.
DR SMR; Q8TBY0; -.
DR BioGRID; 127937; 10.
DR IntAct; Q8TBY0; 9.
DR STRING; 9606.ENSP00000281722; -.
DR iPTMnet; Q8TBY0; -.
DR PhosphoSitePlus; Q8TBY0; -.
DR BioMuta; RBM46; -.
DR DMDM; 74760436; -.
DR EPD; Q8TBY0; -.
DR jPOST; Q8TBY0; -.
DR MassIVE; Q8TBY0; -.
DR MaxQB; Q8TBY0; -.
DR PaxDb; Q8TBY0; -.
DR PeptideAtlas; Q8TBY0; -.
DR PRIDE; Q8TBY0; -.
DR ProteomicsDB; 3801; -.
DR ProteomicsDB; 5566; -.
DR ProteomicsDB; 74047; -. [Q8TBY0-1]
DR Antibodypedia; 16839; 106 antibodies from 20 providers.
DR DNASU; 166863; -.
DR Ensembl; ENST00000281722.8; ENSP00000281722.3; ENSG00000151962.8. [Q8TBY0-1]
DR Ensembl; ENST00000510397.5; ENSP00000422813.1; ENSG00000151962.8. [Q8TBY0-2]
DR Ensembl; ENST00000514866.5; ENSP00000424500.1; ENSG00000151962.8. [Q8TBY0-3]
DR GeneID; 166863; -.
DR KEGG; hsa:166863; -.
DR MANE-Select; ENST00000281722.8; ENSP00000281722.3; NM_144979.5; NP_659416.1.
DR UCSC; uc003ioo.5; human. [Q8TBY0-1]
DR CTD; 166863; -.
DR DisGeNET; 166863; -.
DR GeneCards; RBM46; -.
DR HGNC; HGNC:28401; RBM46.
DR HPA; ENSG00000151962; Tissue enriched (testis).
DR neXtProt; NX_Q8TBY0; -.
DR OpenTargets; ENSG00000151962; -.
DR PharmGKB; PA162400810; -.
DR VEuPathDB; HostDB:ENSG00000151962; -.
DR eggNOG; KOG0117; Eukaryota.
DR GeneTree; ENSGT00940000155927; -.
DR HOGENOM; CLU_022960_5_0_1; -.
DR InParanoid; Q8TBY0; -.
DR OMA; QSYFMPE; -.
DR OrthoDB; 1384330at2759; -.
DR PhylomeDB; Q8TBY0; -.
DR TreeFam; TF314932; -.
DR PathwayCommons; Q8TBY0; -.
DR SignaLink; Q8TBY0; -.
DR BioGRID-ORCS; 166863; 10 hits in 1068 CRISPR screens.
DR GenomeRNAi; 166863; -.
DR Pharos; Q8TBY0; Tbio.
DR PRO; PR:Q8TBY0; -.
DR Proteomes; UP000005640; Chromosome 4.
DR RNAct; Q8TBY0; protein.
DR Bgee; ENSG00000151962; Expressed in secondary oocyte and 61 other tissues.
DR ExpressionAtlas; Q8TBY0; baseline and differential.
DR Genevisible; Q8TBY0; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0048255; P:mRNA stabilization; IEA:Ensembl.
DR GO; GO:0001829; P:trophectodermal cell differentiation; IEA:Ensembl.
DR CDD; cd19901; DSRM_RBM46; 1.
DR CDD; cd12484; RRM1_RBM46; 1.
DR CDD; cd12492; RRM2_RBM46; 1.
DR Gene3D; 3.30.70.330; -; 3.
DR InterPro; IPR006535; HnRNP_R/Q_splicing_fac.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR044462; RBM46_DSR.
DR InterPro; IPR034434; RBM46_RRM1.
DR InterPro; IPR034435; RBM46_RRM2.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 3.
DR SMART; SM00360; RRM; 3.
DR SUPFAM; SSF54928; SSF54928; 3.
DR TIGRFAMs; TIGR01648; hnRNP-R-Q; 1.
DR PROSITE; PS50102; RRM; 3.
PE 1: Evidence at protein level;
KW Alternative splicing; Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..533
FT /note="Probable RNA-binding protein 46"
FT /id="PRO_0000305315"
FT DOMAIN 61..139
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 141..223
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 236..308
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT VAR_SEQ 468..533
FT /note="DYNFHRSSINSLSPVSATLSSGTPSVLPYTSRPYSYPGYPLSPTISLANGSH
FT VGQRLCISNQASFF -> GPF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055251"
FT VAR_SEQ 469..533
FT /note="YNFHRSSINSLSPVSATLSSGTPSVLPYTSRPYSYPGYPLSPTISLANGSHV
FT GQRLCISNQASFF -> REHNLFSLDLCRRIWRK (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055252"
SQ SEQUENCE 533 AA; 60023 MW; A466A5AA76CD8BBE CRC64;
MNEENIDGTN GCSKVRTGIQ NEAALLALME KTGYNMVQEN GQRKFGGPPP GWEGPPPPRG
CEVFVGKIPR DMYEDELVPV FERAGKIYEF RLMMEFSGEN RGYAFVMYTT KEEAQLAIRI
LNNYEIRPGK FIGVCVSLDN CRLFIGAIPK EKKKEEILDE MKKVTEGVVD VIVYPSATDK
TKNRGFAFVE YESHRAAAMA RRKLIPGTFQ LWGHTIQVDW ADPEKEVDEE TMQRVKVLYV
RNLMISTTEE TIKAEFNKFK PGAVERVKKL RDYAFVHFFN REDAVAAMSV MNGKCIDGAS
IEVTLAKPVN KENTWRQHLN GQISPNSENL IVFANKEESH PKTLGKLPTL PARLNGQHSP
SPPEVERCTY PFYPGTKLTP ISMYSLKSNH FNSAVMHLDY YCNKNNWAPP EYYLYSTTSQ
DGKVLLVYKI VIPAIANGSQ SYFMPDKLCT TLEDAKELAA QFTLLHLDYN FHRSSINSLS
PVSATLSSGT PSVLPYTSRP YSYPGYPLSP TISLANGSHV GQRLCISNQA SFF
