RBM47_MOUSE
ID RBM47_MOUSE Reviewed; 590 AA.
AC Q91WT8; Q3UQC7; Q8BZE8;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=RNA-binding protein 47;
DE AltName: Full=RNA-binding motif protein 47;
GN Name=Rbm47;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD;
RC TISSUE=Kidney, Mammary gland, and Urinary bladder;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N, and NMRI; TISSUE=Colon, Kidney, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Kidney, Liver, and Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-396 AND ARG-407, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryo;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q91WT8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q91WT8-2; Sequence=VSP_028840, VSP_028841;
CC -!- SIMILARITY: Belongs to the RRM RBM47 family. {ECO:0000305}.
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DR EMBL; AK035620; BAC29127.1; -; mRNA.
DR EMBL; AK085733; BAC39524.1; -; mRNA.
DR EMBL; AK142584; BAE25115.1; -; mRNA.
DR EMBL; AK170082; BAE41553.1; -; mRNA.
DR EMBL; BC013481; AAH13481.1; -; mRNA.
DR EMBL; BC034195; AAH34195.1; -; mRNA.
DR EMBL; BC112902; AAI12903.1; -; mRNA.
DR CCDS; CCDS19313.1; -. [Q91WT8-1]
DR RefSeq; NP_001120854.1; NM_001127382.2. [Q91WT8-1]
DR RefSeq; NP_001278155.1; NM_001291226.1.
DR RefSeq; NP_620704.1; NM_139065.3. [Q91WT8-1]
DR RefSeq; NP_848541.2; NM_178446.4. [Q91WT8-1]
DR RefSeq; XP_006504019.1; XM_006503956.2. [Q91WT8-1]
DR AlphaFoldDB; Q91WT8; -.
DR SMR; Q91WT8; -.
DR BioGRID; 232855; 15.
DR STRING; 10090.ENSMUSP00000092349; -.
DR iPTMnet; Q91WT8; -.
DR PhosphoSitePlus; Q91WT8; -.
DR jPOST; Q91WT8; -.
DR MaxQB; Q91WT8; -.
DR PaxDb; Q91WT8; -.
DR PeptideAtlas; Q91WT8; -.
DR PRIDE; Q91WT8; -.
DR ProteomicsDB; 255132; -. [Q91WT8-1]
DR ProteomicsDB; 255133; -. [Q91WT8-2]
DR Antibodypedia; 1338; 46 antibodies from 20 providers.
DR DNASU; 245945; -.
DR Ensembl; ENSMUST00000094757; ENSMUSP00000092349; ENSMUSG00000070780. [Q91WT8-1]
DR Ensembl; ENSMUST00000113724; ENSMUSP00000109353; ENSMUSG00000070780. [Q91WT8-1]
DR Ensembl; ENSMUST00000113726; ENSMUSP00000109355; ENSMUSG00000070780. [Q91WT8-1]
DR Ensembl; ENSMUST00000167950; ENSMUSP00000128016; ENSMUSG00000070780. [Q91WT8-1]
DR Ensembl; ENSMUST00000200775; ENSMUSP00000144325; ENSMUSG00000070780. [Q91WT8-1]
DR Ensembl; ENSMUST00000201544; ENSMUSP00000144122; ENSMUSG00000070780. [Q91WT8-1]
DR GeneID; 245945; -.
DR KEGG; mmu:245945; -.
DR UCSC; uc008xok.3; mouse. [Q91WT8-1]
DR UCSC; uc008xop.3; mouse. [Q91WT8-2]
DR CTD; 54502; -.
DR MGI; MGI:2384294; Rbm47.
DR VEuPathDB; HostDB:ENSMUSG00000070780; -.
DR eggNOG; KOG0117; Eukaryota.
DR GeneTree; ENSGT00940000156979; -.
DR HOGENOM; CLU_022960_4_2_1; -.
DR InParanoid; Q91WT8; -.
DR OMA; APNIQRI; -.
DR OrthoDB; 1384330at2759; -.
DR PhylomeDB; Q91WT8; -.
DR TreeFam; TF314932; -.
DR BioGRID-ORCS; 245945; 7 hits in 73 CRISPR screens.
DR ChiTaRS; Rbm47; mouse.
DR PRO; PR:Q91WT8; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q91WT8; protein.
DR Bgee; ENSMUSG00000070780; Expressed in epithelium of small intestine and 181 other tissues.
DR ExpressionAtlas; Q91WT8; baseline and differential.
DR Genevisible; Q91WT8; MM.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IDA:MGI.
DR GO; GO:0016554; P:cytidine to uridine editing; IGI:MGI.
DR GO; GO:0002244; P:hematopoietic progenitor cell differentiation; IMP:MGI.
DR CDD; cd12491; RRM2_RBM47; 1.
DR CDD; cd12497; RRM3_RBM47; 1.
DR Gene3D; 3.30.70.330; -; 3.
DR InterPro; IPR006535; HnRNP_R/Q_splicing_fac.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR034442; RBM47.
DR InterPro; IPR034440; RBM47_RRM2.
DR InterPro; IPR034445; RBM47_RRM3.
DR InterPro; IPR000504; RRM_dom.
DR PANTHER; PTHR21245:SF6; PTHR21245:SF6; 1.
DR Pfam; PF00076; RRM_1; 3.
DR SMART; SM00360; RRM; 3.
DR SUPFAM; SSF54928; SSF54928; 2.
DR TIGRFAMs; TIGR01648; hnRNP-R-Q; 1.
DR PROSITE; PS50102; RRM; 3.
PE 1: Evidence at protein level;
KW Alternative splicing; Methylation; Nucleus; Reference proteome; Repeat;
KW RNA-binding.
FT CHAIN 1..590
FT /note="RNA-binding protein 47"
FT /id="PRO_0000307856"
FT DOMAIN 71..149
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 151..233
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 246..318
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 396
FT /note="Asymmetric dimethylarginine; alternate"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 396
FT /note="Omega-N-methylarginine; alternate"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 407
FT /note="Asymmetric dimethylarginine; alternate"
FT /evidence="ECO:0000250|UniProtKB:A0AV96"
FT MOD_RES 407
FT /note="Omega-N-methylarginine; alternate"
FT /evidence="ECO:0007744|PubMed:24129315"
FT VAR_SEQ 377
FT /note="T -> S (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028840"
FT VAR_SEQ 378..590
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028841"
FT CONFLICT 557
FT /note="K -> E (in Ref. 1; BAC29127)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 590 AA; 64062 MW; B74421B7EC014E0A CRC64;
MTAEDSATAM NSDPTVGSST KVPEGVAGAP NEAALLALIE RTGYTMVQEN GQRKYGGPPP
GWEGPHPQRG CEVFVGKIPR DVYEDELVPV FETVGRIYEL RLMMDFDGKN RGYAFVMYCH
KHEAKRAVRE LNNYEIRPGR LLGVCCSVDN CRLFIGGIPK MKKRGEILEE IAKVTEGVLN
VIVYASAADK MKNRGFAFVE YESHRAAAMA RRKLMPGRIQ LWGHQIAVDW AEPEIDVDED
VMQTVKILYV RNLMIETTEE TIKKSFGQFN PGCVERVKKI RDYAFVHFTS REDAVHAMNN
LNGTELEGSC LEVTLAKPVD KEQYSRYQKA AKGGGGSAEA VAQQPSYVYS CDPYTLAYYG
YPYNALIGPN RDYFVKTGSI RGRGRGAAGN RTPGPRGSYL GGYSAGRGIY SRYHEGKGKQ
QEKGYELVPN LEISPVNPVA IKPGTVAIPA IGAQYSMFQA APAPKIIEDG KIHTMEHMIS
PIAVQPDPAT AAAAAAAAAA AAVIPAVSTP PPFQGRPITP VYTVAPNVQR IPTAGIYGAS
YVPFAAPATA TIATLQKNAA AAVYGGYAGY IPQAFPAALQ VPIHDVYQTY
