RBM5_XENTR
ID RBM5_XENTR Reviewed; 838 AA.
AC A4IGK4; Q28D22;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=RNA-binding protein 5;
DE AltName: Full=RNA-binding motif protein 5;
GN Name=rbm5; ORFNames=TGas113j08.1;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Gastrula;
RG Sanger Xenopus tropicalis EST/cDNA project;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the spliceosome A complex. Regulates alternative
CC splicing of a number of mRNAs. May modulate splice site pairing after
CC recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of
CC the intron (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the spliceosome A complex (also known as the
CC prespliceosome). Appears to dissociate from the spliceosome upon
CC formation of the spliceosome B complex (also known as the precatalytic
CC spliceosome), in which the heterotrimeric U4/U6.U5 snRNPs are bound (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the RBM5/RBM10 family. {ECO:0000305}.
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DR EMBL; CR855749; CAJ81574.1; -; mRNA.
DR EMBL; BC135140; AAI35141.1; -; mRNA.
DR RefSeq; NP_001017278.1; NM_001017278.2.
DR RefSeq; XP_012820244.1; XM_012964790.2.
DR AlphaFoldDB; A4IGK4; -.
DR PRIDE; A4IGK4; -.
DR DNASU; 550032; -.
DR Ensembl; ENSXETT00000015911; ENSXETP00000015911; ENSXETG00000007303.
DR GeneID; 550032; -.
DR KEGG; xtr:550032; -.
DR CTD; 10181; -.
DR Xenbase; XB-GENE-494987; rbm5.
DR HOGENOM; CLU_010527_0_0_1; -.
DR InParanoid; A4IGK4; -.
DR OMA; TIMGVIR; -.
DR OrthoDB; 786674at2759; -.
DR PhylomeDB; A4IGK4; -.
DR Reactome; R-XTR-72163; mRNA Splicing - Major Pathway.
DR Proteomes; UP000008143; Chromosome 4.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000007303; Expressed in brain and 27 other tissues.
DR ExpressionAtlas; A4IGK4; baseline.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003729; F:mRNA binding; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0043065; P:positive regulation of apoptotic process; ISS:UniProtKB.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0000245; P:spliceosomal complex assembly; ISS:UniProtKB.
DR CDD; cd12752; RRM1_RBM5; 1.
DR CDD; cd12755; RRM2_RBM5; 1.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR000467; G_patch_dom.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR041591; OCRE.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR033094; RBM5.
DR InterPro; IPR034991; RBM5_RRM1.
DR InterPro; IPR034993; RBM5_RRM2.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR013087; Znf_C2H2_type.
DR InterPro; IPR001876; Znf_RanBP2.
DR InterPro; IPR036443; Znf_RanBP2_sf.
DR PANTHER; PTHR13948:SF21; PTHR13948:SF21; 1.
DR Pfam; PF01585; G-patch; 1.
DR Pfam; PF17780; OCRE; 1.
DR Pfam; PF00076; RRM_1; 1.
DR Pfam; PF00641; zf-RanBP; 1.
DR SMART; SM00443; G_patch; 1.
DR SMART; SM00360; RRM; 2.
DR SMART; SM00547; ZnF_RBZ; 1.
DR SUPFAM; SSF54928; SSF54928; 2.
DR SUPFAM; SSF90209; SSF90209; 1.
DR PROSITE; PS50174; G_PATCH; 1.
DR PROSITE; PS50102; RRM; 2.
DR PROSITE; PS01358; ZF_RANBP2_1; 1.
DR PROSITE; PS50199; ZF_RANBP2_2; 1.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 1.
PE 2: Evidence at transcript level;
KW Metal-binding; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Repeat; RNA-binding; Spliceosome; Zinc; Zinc-finger.
FT CHAIN 1..838
FT /note="RNA-binding protein 5"
FT /id="PRO_0000376808"
FT DOMAIN 103..183
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 242..326
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 766..812
FT /note="G-patch"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00092"
FT ZN_FING 186..215
FT /note="RanBP2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00322"
FT ZN_FING 671..696
FT /note="C2H2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 396..473
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 524..561
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 396..459
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 543..561
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 192
FT /note="C -> S (in Ref. 1; CAJ81574)"
FT /evidence="ECO:0000305"
FT CONFLICT 237..243
FT /note="SGYVSSA -> T (in Ref. 1; CAJ81574)"
FT /evidence="ECO:0000305"
FT CONFLICT 610
FT /note="Missing (in Ref. 1; CAJ81574)"
FT /evidence="ECO:0000305"
FT CONFLICT 659
FT /note="A -> V (in Ref. 1; CAJ81574)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 838 AA; 94499 MW; EB0586C08496D8BB CRC64;
MGSDKRVSRS ERSGRYGSAF DRDDRDDRDT RSRRRDSEYK RYRDERSSER YDDYRDYDSP
ERDRMRDRER RNSDRSEDGY HSDGDYMDHD YRQDYYMDEK ESKTIMLRGL PININENDIR
ELVESFEGPQ PADVRLMKRK TGLSRGFAFV EFYHLQDATR WMEANQKKLV IQGKTIAMHY
SNPRPKFEDW LCNKCGLYNF RRRLKCFRCG AAKAESDLEA PSGSSDAPQS TDYYSDSGYV
SSAIILRNIG PHTVVDSILS ALAPYVSLVV SNIRLIKDKQ TQQNRGFAFV QLPSTLEASQ
LLQILQTLHP PLKIDGKTVG VDFAKSARKD LVLPDGHRVS AFSVASTAIA AAQWSSTQQA
QQSGEGGEYA YLQPGQEGYA NYGQCSQDYQ PFYQAQTGAA EQSTAPQAEG SAPVPATTSA
VVCQSPQMYQ QPGSPTQSGT STAANTTPAS TTSTTEEAAP PNAVIPGVKY SVPDTSTYQY
DESSGYYYDP QTGLYYDPNS QYYYNSLTQQ YLYWDGEKQT YLPAADGAGQ SGAQPNGANA
GSSKEGKEKK EKPKSKTAQQ IAKDMERWAK SLNKQKENFK NSFQPLSARD EERKESAAAD
AGFALFEKKQ GALLERQFMP DMMMMVNTEE EEKPPNKALV AAYSGDSDNE EESERLLGAV
DEEKLLDWKK LACLLCRRQF PNKDALTRHQ QLSDLHKQNL EVYRRSKMSE QELEALELKE
REAKYRDRAA ERREKYGIPE PPEPKRKRFD PTVVNYEQPT KDGIDNSNIG NKMLQAMGWK
EGSGLGRKSQ GITAPIQAQV RMRGAGLGAK GSSYGVNTSD SYKDAVRKAM FARFSEME
