RBM8A_BOVIN
ID RBM8A_BOVIN Reviewed; 174 AA.
AC Q3ZCE8; Q1JPH7;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=RNA-binding protein 8A;
DE AltName: Full=RNA-binding motif protein 8A;
GN Name=RBM8A;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT "Characterization of 954 bovine full-CDS cDNA sequences.";
RL BMC Genomics 6:166-166(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Required for pre-mRNA splicing as component of the
CC spliceosome (By similarity). Core component of the splicing-dependent
CC multiprotein exon junction complex (EJC) deposited at splice junctions
CC on mRNAs. The EJC is a dynamic structure consisting of core proteins
CC and several peripheral nuclear and cytoplasmic associated factors that
CC join the complex only transiently either during EJC assembly or during
CC subsequent mRNA metabolism. The EJC marks the position of the exon-exon
CC junction in the mature mRNA for the gene expression machinery and the
CC core components remain bound to spliced mRNAs throughout all stages of
CC mRNA metabolism thereby influencing downstream processes including
CC nuclear mRNA export, subcellular mRNA localization, translation
CC efficiency and nonsense-mediated mRNA decay (NMD). Its removal from
CC cytoplasmic mRNAs requires translation initiation from EJC-bearing
CC spliced mRNAs. Associates preferentially with mRNAs produced by
CC splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced
CC from intronless cDNAs. Associates with both nuclear mRNAs and newly
CC exported cytoplasmic mRNAs (By similarity).
CC {ECO:0000250|UniProtKB:Q9Y5S9}.
CC -!- SUBUNIT: Heterodimer with either MAGOH or MAGOHB. Part of the mRNA
CC splicing-dependent exon junction complex (EJC) complex; the core
CC complex contains CASC3, EIF4A3, MAGOH or MAGOHB, and RBM8A. Interacts
CC with PYM1; the interaction is direct and dissociates the EJC from
CC spliced mRNAs. Part of a complex that contains the EJC core components
CC CASC3, EIF4A3, MAGOH and RBM8A plus proteins involved in nonsense-
CC mediated mRNA decay, such as UPF1, UPF2, UPF3A and UPF3B. Found in a
CC post-splicing complex with NXF1, MAGOH, UPF1, UPF2, UPF3A, UPF3B and
CC RNPS1. Interacts with DDX39B, MAGOH, DPH1, UPF3B, RNPS1, SRRM1 and
CC ALYREF/THOC4. Interacts with IPO13; the interaction mediates the
CC nuclear import of the MAGOH-RBM8A heterodimer. Identified in the
CC spliceosome C complex. Associates with polysomes.
CC {ECO:0000250|UniProtKB:Q9Y5S9}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9Y5S9}. Nucleus
CC speckle {ECO:0000250|UniProtKB:Q9Y5S9}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q9Y5S9}. Note=Nucleocytoplasmic shuttling
CC protein. Travels to the cytoplasm as part of the exon junction complex
CC (EJC) bound to mRNA. Colocalizes with the core EJC, ALYREF/THOC4, NXF1
CC and UAP56 in the nucleus and nuclear speckles.
CC {ECO:0000250|UniProtKB:Q9Y5S9}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3ZCE8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3ZCE8-2; Sequence=VSP_037600;
CC -!- SIMILARITY: Belongs to the RBM8A family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BT025376; ABF57332.1; -; mRNA.
DR EMBL; BC102484; AAI02485.1; -; mRNA.
DR RefSeq; NP_001030401.1; NM_001035324.2. [Q3ZCE8-2]
DR RefSeq; XP_015318708.1; XM_015463222.1. [Q3ZCE8-1]
DR AlphaFoldDB; Q3ZCE8; -.
DR SMR; Q3ZCE8; -.
DR STRING; 9913.ENSBTAP00000011015; -.
DR PaxDb; Q3ZCE8; -.
DR PeptideAtlas; Q3ZCE8; -.
DR PRIDE; Q3ZCE8; -.
DR GeneID; 518045; -.
DR KEGG; bta:518045; -.
DR CTD; 9939; -.
DR eggNOG; KOG0130; Eukaryota.
DR HOGENOM; CLU_012062_18_3_1; -.
DR InParanoid; Q3ZCE8; -.
DR OrthoDB; 1234149at2759; -.
DR TreeFam; TF314933; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0035145; C:exon-exon junction complex; IBA:GO_Central.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; ISS:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IBA:GO_Central.
DR CDD; cd12324; RRM_RBM8; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR008111; RNA-bd_8.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR033744; RRM_RBM8.
DR PANTHER; PTHR45894; PTHR45894; 1.
DR Pfam; PF00076; RRM_1; 1.
DR PRINTS; PR01738; RNABINDINGM8.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Alternative splicing; Cytoplasm; Isopeptide bond;
KW mRNA processing; mRNA splicing; mRNA transport;
KW Nonsense-mediated mRNA decay; Nucleus; Phosphoprotein; Reference proteome;
KW RNA-binding; Spliceosome; Translation regulation; Transport;
KW Ubl conjugation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9Y5S9"
FT CHAIN 2..174
FT /note="RNA-binding protein 8A"
FT /id="PRO_0000282605"
FT DOMAIN 73..151
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 151..174
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..29
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 36..65
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 156..174
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y5S9"
FT MOD_RES 24
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y5S9"
FT MOD_RES 42
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y5S9"
FT MOD_RES 56
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y5S9"
FT CROSSLNK 27
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9Y5S9"
FT VAR_SEQ 43
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_037600"
SQ SEQUENCE 174 AA; 19889 MW; 70BBD03CDDFEECFE CRC64;
MADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM REDYDSVEQD
GDEPGPQRSV EGWILFVTGV HEEATEEDIH DKFAEYGEIK NIHLNLDRRT GYLKGYTLVE
YETYKEAQAA MEGLNGQDLM GQPISVDWCF VRGPPKGKRR GGRRRSRSPD RRRR
