RBM8A_DANRE
ID RBM8A_DANRE Reviewed; 174 AA.
AC Q5D018;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 29-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=RNA-binding protein 8A;
DE AltName: Full=RNA-binding motif protein 8A;
DE AltName: Full=Ribonucleoprotein RBM8A;
GN Name=rbm8a; Synonyms=rbm8; ORFNames=zgc:110525;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Required for pre-mRNA splicing as component of the
CC spliceosome (By similarity). Core component of the splicing-dependent
CC multiprotein exon junction complex (EJC) deposited at splice junctions
CC on mRNAs. The EJC is a dynamic structure consisting of core proteins
CC and several peripheral nuclear and cytoplasmic associated factors that
CC join the complex only transiently either during EJC assembly or during
CC subsequent mRNA metabolism. The EJC marks the position of the exon-exon
CC junction in the mature mRNA for the gene expression machinery and the
CC core components remain bound to spliced mRNAs throughout all stages of
CC mRNA metabolism thereby influencing downstream processes including
CC nuclear mRNA export, subcellular mRNA localization, translation
CC efficiency and nonsense-mediated mRNA decay (NMD). Its removal from
CC cytoplasmic mRNAs requires translation initiation from EJC-bearing
CC spliced mRNAs. Associates preferentially with mRNAs produced by
CC splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced
CC from intronless cDNAs. Associates with both nuclear mRNAs and newly
CC exported cytoplasmic mRNAs (By similarity).
CC {ECO:0000250|UniProtKB:Q9Y5S9}.
CC -!- SUBUNIT: Part of the EJC core complex that contains casc3, eif4a3,
CC magoh and rbm8a. Identified in the spliceosome C complex.
CC {ECO:0000250|UniProtKB:Q9Y5S9}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9Y5S9}. Nucleus
CC speckle {ECO:0000250|UniProtKB:Q9Y5S9}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q9Y5S9}. Note=Nucleocytoplasmic shuttling
CC protein. Travels to the cytoplasm as part of the exon junction complex
CC (EJC) bound to mRNA. {ECO:0000250|UniProtKB:Q9Y5S9}.
CC -!- SIMILARITY: Belongs to the RBM8A family. {ECO:0000305}.
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DR EMBL; BC090261; AAH90261.1; -; mRNA.
DR RefSeq; NP_001013363.1; NM_001013345.1.
DR AlphaFoldDB; Q5D018; -.
DR SMR; Q5D018; -.
DR STRING; 7955.ENSDARP00000026575; -.
DR PaxDb; Q5D018; -.
DR PRIDE; Q5D018; -.
DR Ensembl; ENSDART00000018253; ENSDARP00000026575; ENSDARG00000016516.
DR GeneID; 503767; -.
DR KEGG; dre:503767; -.
DR CTD; 9939; -.
DR ZFIN; ZDB-GENE-050306-51; rbm8a.
DR eggNOG; KOG0130; Eukaryota.
DR GeneTree; ENSGT00730000111185; -.
DR HOGENOM; CLU_012062_18_3_1; -.
DR InParanoid; Q5D018; -.
DR OMA; TIAVDWC; -.
DR OrthoDB; 1444995at2759; -.
DR PhylomeDB; Q5D018; -.
DR TreeFam; TF314933; -.
DR Reactome; R-DRE-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-DRE-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-DRE-72187; mRNA 3'-end processing.
DR Reactome; R-DRE-73856; RNA Polymerase II Transcription Termination.
DR Reactome; R-DRE-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR PRO; PR:Q5D018; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 16.
DR Bgee; ENSDARG00000016516; Expressed in mature ovarian follicle and 28 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0035145; C:exon-exon junction complex; IBA:GO_Central.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; ISS:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; IEA:InterPro.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IBA:GO_Central.
DR CDD; cd12324; RRM_RBM8; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR008111; RNA-bd_8.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR033744; RRM_RBM8.
DR PANTHER; PTHR45894; PTHR45894; 1.
DR Pfam; PF00076; RRM_1; 1.
DR PRINTS; PR01738; RNABINDINGM8.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; mRNA processing; mRNA splicing; mRNA transport;
KW Nonsense-mediated mRNA decay; Nucleus; Reference proteome; RNA-binding;
KW Spliceosome; Translation regulation; Transport.
FT CHAIN 1..174
FT /note="RNA-binding protein 8A"
FT /id="PRO_0000378564"
FT DOMAIN 73..151
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 151..174
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..29
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 36..65
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 156..174
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 174 AA; 19881 MW; A6FD972B472D25A7 CRC64;
MADVLDLHEA GGEDFPMDED GDESIHKLKE KAKKRKGRGF GSEEGARSRV REDYDTVEQD
GDEPGPQRSV EGWILFVTGV HEEATEEDVH DKFAEFGEIK NLHLNLDRRT GYLKGYALVE
YETYKEAQAA MEGLNGQELM GQPISVDWCF VRGPPKSKRR GGRRRSRSPD RRRR
