RBMS1_MOUSE
ID RBMS1_MOUSE Reviewed; 403 AA.
AC Q91W59; Q3UI25; Q3UXU2; Q6PEU6; Q6PHC2; Q9WTK4;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=RNA-binding motif, single-stranded-interacting protein 1;
DE AltName: Full=Single-stranded DNA-binding protein MSSP-1;
GN Name=Rbms1; Synonyms=Mssp, Mssp1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2), AND TISSUE
RP SPECIFICITY.
RC TISSUE=Brain;
RX PubMed=10639566; DOI=10.3892/ijo.16.2.245;
RA Fujimoto M., Matsumoto K., Iguchi-Ariga S.M.M., Ariga H.;
RT "Structure and comparison of genomic and complementary DNAs of mouse MSSP,
RT a c-Myc binding protein.";
RL Int. J. Oncol. 16:245-251(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Heart, and Wolffian duct;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=C57BL/6J, FVB/N, and NMRI; TISSUE=Mammary gland, and Retina;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Single-stranded DNA binding protein that interacts with the
CC region upstream of the MYC gene. Binds specifically to the DNA sequence
CC motif 5'-[AT]CT[AT][AT]T-3'. Probably has a role in DNA replication.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q91W59-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q91W59-2; Sequence=VSP_011931;
CC Name=3;
CC IsoId=Q91W59-3; Sequence=VSP_011931, VSP_011932;
CC -!- TISSUE SPECIFICITY: Ubiquitous. Expressed in all tissues except testis.
CC {ECO:0000269|PubMed:10639566}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH16501.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB026582; BAA77264.1; -; Genomic_DNA.
DR EMBL; AB026569; BAA77262.1; -; mRNA.
DR EMBL; AK135280; BAE22471.1; -; mRNA.
DR EMBL; AK147109; BAE27681.1; -; mRNA.
DR EMBL; AL928581; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL929012; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC016501; AAH16501.2; ALT_INIT; mRNA.
DR EMBL; BC056609; AAH56609.1; -; mRNA.
DR EMBL; BC057866; AAH57866.1; -; mRNA.
DR CCDS; CCDS50591.1; -. [Q91W59-1]
DR RefSeq; NP_001135403.1; NM_001141931.1.
DR RefSeq; NP_064692.2; NM_020296.2. [Q91W59-1]
DR AlphaFoldDB; Q91W59; -.
DR SMR; Q91W59; -.
DR BioGRID; 208206; 3.
DR IntAct; Q91W59; 1.
DR STRING; 10090.ENSMUSP00000028347; -.
DR iPTMnet; Q91W59; -.
DR PhosphoSitePlus; Q91W59; -.
DR EPD; Q91W59; -.
DR MaxQB; Q91W59; -.
DR PaxDb; Q91W59; -.
DR PeptideAtlas; Q91W59; -.
DR PRIDE; Q91W59; -.
DR ProteomicsDB; 300270; -. [Q91W59-1]
DR ProteomicsDB; 300271; -. [Q91W59-2]
DR ProteomicsDB; 300272; -. [Q91W59-3]
DR Antibodypedia; 19068; 306 antibodies from 28 providers.
DR DNASU; 56878; -.
DR Ensembl; ENSMUST00000028347; ENSMUSP00000028347; ENSMUSG00000026970. [Q91W59-1]
DR Ensembl; ENSMUST00000112509; ENSMUSP00000108128; ENSMUSG00000026970. [Q91W59-3]
DR GeneID; 56878; -.
DR KEGG; mmu:56878; -.
DR UCSC; uc008juq.2; mouse. [Q91W59-1]
DR CTD; 5937; -.
DR MGI; MGI:1861774; Rbms1.
DR VEuPathDB; HostDB:ENSMUSG00000026970; -.
DR eggNOG; KOG4733; Eukaryota.
DR GeneTree; ENSGT00940000156082; -.
DR HOGENOM; CLU_016278_2_0_1; -.
DR InParanoid; Q91W59; -.
DR OMA; WEHLSKT; -.
DR OrthoDB; 810893at2759; -.
DR PhylomeDB; Q91W59; -.
DR TreeFam; TF314644; -.
DR BioGRID-ORCS; 56878; 4 hits in 73 CRISPR screens.
DR ChiTaRS; Rbms1; mouse.
DR PRO; PR:Q91W59; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q91W59; protein.
DR Bgee; ENSMUSG00000026970; Expressed in placenta labyrinth and 258 other tissues.
DR ExpressionAtlas; Q91W59; baseline and differential.
DR Genevisible; Q91W59; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IBA:GO_Central.
DR GO; GO:0008143; F:poly(A) binding; IBA:GO_Central.
DR GO; GO:0008266; F:poly(U) RNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd12470; RRM1_MSSP1; 1.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR002343; Hud_Sxl_RNA.
DR InterPro; IPR034404; MSSP1_RRM1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 2.
DR PRINTS; PR00961; HUDSXLRNA.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA replication; DNA-binding; Nucleus;
KW Phosphoprotein; Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..403
FT /note="RNA-binding motif, single-stranded-interacting
FT protein 1"
FT /id="PRO_0000081799"
FT DOMAIN 62..135
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 141..226
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 30..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 382..403
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..56
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 208
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P29558"
FT VAR_SEQ 1..33
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:10639566,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_011931"
FT VAR_SEQ 298
FT /note="V -> VAKETRENKYRGSAIKV (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_011932"
FT CONFLICT 158
FT /note="E -> K (in Ref. 2; BAE22471)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 403 AA; 43991 MW; 68B8AC5816834726 CRC64;
MGKVWKQQMY PQYATYYYPQ YLQAKQSLVP AHPMAPPSPS TTSSNNNSSS SSNSGWDQLS
KTNLYIRGLP PNTTDQDLVK LCQPYGKIVS TKAILDKATN KCKGYGFVDF DSPAAAQKAV
SALKANGVQA QMAKQQEQDP TNLYISNLPL SMDEQELENM LKPFGQVIST RVLRDSSGAS
RGVGFARMES TEKCEAVIGH FNGKFIKTPP GVSAPTEPLL CKFADGGQKK RQNPNKYIPN
GRPWPRDGEA GMTLTYDPTT AALHNGFYPS PYSIATNRMI TQTSLTPYIA SPVSAYQVQS
PSWMQPQPYI LQHPGAVLTP SMEHTMSLQP ASMISPLAQQ MSHLSLGSTG TYMPATSAMQ
GAYLPQYTHM QTAAVPVEEA SGQQQVAVET SNDHSPYTFP PNK
