RBMX_DANRE
ID RBMX_DANRE Reviewed; 379 AA.
AC Q7ZWA3; Q6TLG7;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=RNA-binding motif protein, X chromosome;
DE AltName: Full=Heterogeneous nuclear ribonucleoprotein G;
DE Short=hnRNP G;
GN Name=rbmx;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, AND DEVELOPMENTAL STAGE.
RC TISSUE=Ovary;
RX PubMed=15895365; DOI=10.1002/dvdy.20432;
RA Tsend-Ayush E., O'Sullivan L.A., Grutzner F.S., Onnebo S.M., Lewis R.S.,
RA Delbridge M.L., Graves J.A., Ward A.C.;
RT "RBMX gene is essential for brain development in zebrafish.";
RL Dev. Dyn. 234:682-688(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Kidney marrow;
RX PubMed=15520368; DOI=10.1073/pnas.0407241101;
RA Song H.-D., Sun X.-J., Deng M., Zhang G.-W., Zhou Y., Wu X.-Y., Sheng Y.,
RA Chen Y., Ruan Z., Jiang C.-L., Fan H.-Y., Zon L.I., Kanki J.P., Liu T.X.,
RA Look A.T., Chen Z.;
RT "Hematopoietic gene expression profile in zebrafish kidney marrow.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: RNA-binding protein that plays several role in the regulation
CC of pre- and post-transcriptional processes. Implicated in tissue-
CC specific regulation of gene transcription and alternative splicing of
CC pre-mRNAs. Associates with chromatin. Associates with nascent mRNAs
CC transcribed by RNA polymerase II. Component of the supraspliceosome
CC complex that regulates pre-mRNA alternative splice site selection.
CC Binds non-specifically to pre-mRNAs (By similarity). Required for
CC embryonic development, in particular of the brain. {ECO:0000250,
CC ECO:0000269|PubMed:15895365}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7ZWA3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7ZWA3-2; Sequence=VSP_041850;
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Expressed widely in the embryo up to 24 hr post-fertilization (hpf).
CC Expressed predominantly in the brain, branchial arches, and liver
CC primordium between 24 to 72 hpf. Expressed in the midbrain at 7 days
CC post-fertilization (dpf). {ECO:0000269|PubMed:15895365}.
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DR EMBL; AJ717349; CAG30733.1; -; mRNA.
DR EMBL; AY394938; AAQ94565.1; -; mRNA.
DR EMBL; AL929434; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC049509; AAH49509.1; -; mRNA.
DR EMBL; BC071326; AAH71326.1; -; mRNA.
DR RefSeq; NP_997763.1; NM_212598.1. [Q7ZWA3-1]
DR AlphaFoldDB; Q7ZWA3; -.
DR SMR; Q7ZWA3; -.
DR STRING; 7955.ENSDARP00000009865; -.
DR PaxDb; Q7ZWA3; -.
DR PRIDE; Q7ZWA3; -.
DR Ensembl; ENSDART00000128730; ENSDARP00000111610; ENSDARG00000014244. [Q7ZWA3-1]
DR Ensembl; ENSDART00000138084; ENSDARP00000125065; ENSDARG00000014244. [Q7ZWA3-2]
DR Ensembl; ENSDART00000186597; ENSDARP00000145351; ENSDARG00000014244. [Q7ZWA3-1]
DR GeneID; 321860; -.
DR KEGG; dre:321860; -.
DR CTD; 27316; -.
DR ZFIN; ZDB-GENE-030131-579; rbmx.
DR eggNOG; ENOG502QS9N; Eukaryota.
DR GeneTree; ENSGT00940000165126; -.
DR InParanoid; Q7ZWA3; -.
DR OMA; AKYHEGF; -.
DR OrthoDB; 1248417at2759; -.
DR TreeFam; TF331833; -.
DR PRO; PR:Q7ZWA3; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 14.
DR Bgee; ENSDARG00000014244; Expressed in gastrula and 43 other tissues.
DR ExpressionAtlas; Q7ZWA3; baseline and differential.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; ISS:UniProtKB.
DR GO; GO:0000791; C:euchromatin; ISS:UniProtKB.
DR GO; GO:0070062; C:extracellular exosome; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0044530; C:supraspliceosomal complex; ISS:UniProtKB.
DR GO; GO:0005689; C:U12-type spliceosomal complex; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0017069; F:snRNA binding; IBA:GO_Central.
DR GO; GO:0071347; P:cellular response to interleukin-1; ISS:UniProtKB.
DR GO; GO:0006509; P:membrane protein ectodomain proteolysis; ISS:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0048025; P:negative regulation of mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0048026; P:positive regulation of mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0051260; P:protein homooligomerization; ISS:UniProtKB.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0006366; P:transcription by RNA polymerase II; ISS:UniProtKB.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR003954; RRM_dom_euk.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SMART; SM00361; RRM_1; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; mRNA processing;
KW mRNA splicing; Nucleus; Reference proteome; Ribonucleoprotein; RNA-binding;
KW Spliceosome; Transcription.
FT CHAIN 1..379
FT /note="RNA-binding motif protein, X chromosome"
FT /id="PRO_0000413020"
FT DOMAIN 8..86
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 57..379
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 61..75
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 138..159
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 170..221
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 291..328
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 329..345
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 364..379
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 278..379
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_041850"
FT CONFLICT 60
FT /note="G -> S (in Ref. 1; AAQ94565)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 379 AA; 41621 MW; BF71F574116B605A CRC64;
MAEADRPGKL FIGGLNTETS EKVLEAYFSK FGRISEVLLM KDRETNKSRG FAFVTYENPG
DAKDAAREMN GKPLDGKPIK VEQATKPQFE TSGRRGPPPP RSRGPSRGLR GSRGGPSGMR
GPPSREPFFK GMSSRGPPPM KRGPPERNGG PPPKRSAPSG PMGRPSRDRD PYGPPPRRDS
LMSRRDDGPP HRDDHYGSKD SYSSRDYMSS RDSRDYGPPP RDYPYREYSG HSSSRDDYGS
GSRGYSDRDG YGGGREPRGY IDRPSAGSYR DPYDGYGNSR SAPPSRGPPP SYSGSGGSSR
YDDYGSSSRD GYGSRDSYPS SRSDPYSTNR GDRPGRQERG PPPLERGYPR EYSSSSRGAP
RGGGRGGRRP DRGMGRNRY
