RBP5_BPT5
ID RBP5_BPT5 Reviewed; 640 AA.
AC P23207; Q6QGD0;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 2.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Receptor-binding protein pb5 {ECO:0000305};
DE Short=RBP-pb5 {ECO:0000305};
DE AltName: Full=Tail protein pb5;
GN Name=oad;
GN ORFNames=T5.157 {ECO:0000312|EMBL:AAS77196.1},
GN T5p153 {ECO:0000312|EMBL:AAU05292.1};
OS Escherichia phage T5 (Enterobacteria phage T5).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus.
OX NCBI_TaxID=2695836;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1825083; DOI=10.1128/jb.173.3.1287-1297.1991;
RA Krauel V., Heller K.J.;
RT "Cloning, sequencing, and recombinational analysis with bacteriophage BF23
RT of the bacteriophage T5 oad gene encoding the receptor-binding protein.";
RL J. Bacteriol. 173:1287-1297(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RA Ksenzenko V.N., Kaliman A.V., Krutilina A.I., Shlyapnikov M.G.;
RT "Bacteriophage T5 complete genome.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 11303-B5;
RX PubMed=15661140; DOI=10.1016/j.virol.2004.10.049;
RA Wang J., Jiang Y., Vincent M., Sun Y., Yu H., Wang J., Bao Q., Kong H.,
RA Hu S.;
RT "Complete genome sequence of bacteriophage T5.";
RL Virology 332:45-65(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SUBCELLULAR LOCATION.
RC STRAIN=St0 deletion mutant;
RX PubMed=24198424; DOI=10.1128/jvi.02262-13;
RA Zivanovic Y., Confalonieri F., Ponchon L., Lurz R., Chami M., Flayhan A.,
RA Renouard M., Huet A., Decottignies P., Davidson A.R., Breyton C.,
RA Boulanger P.;
RT "Insights into bacteriophage T5 structure from analysis of its
RT morphogenesis genes and protein components.";
RL J. Virol. 88:1162-1174(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
RX PubMed=8057856; DOI=10.1111/j.1365-2958.1994.tb01020.x;
RA Decker K., Krauel V., Meesmann A., Heller K.J.;
RT "Lytic conversion of Escherichia coli by bacteriophage T5: blocking of the
RT FhuA receptor protein by a lipoprotein expressed early during infection.";
RL Mol. Microbiol. 12:321-332(1994).
RN [6]
RP INTERACTION WITH HOST FHUA RECEPTOR, SUBUNIT, AND FUNCTION.
RX PubMed=12051859; DOI=10.1016/s0022-2836(02)00089-x;
RA Plancon L., Janmot C., le Maire M., Desmadril M., Bonhivers M.,
RA Letellier L., Boulanger P.;
RT "Characterization of a high-affinity complex between the bacterial outer
RT membrane protein FhuA and the phage T5 protein pb5.";
RL J. Mol. Biol. 318:557-569(2002).
RN [7]
RP FUNCTION.
RX PubMed=22659573; DOI=10.1016/j.biochi.2012.05.021;
RA Flayhan A., Wien F., Paternostre M., Boulanger P., Breyton C.;
RT "New insights into pb5, the receptor binding protein of bacteriophage T5,
RT and its interaction with its Escherichia coli receptor FhuA.";
RL Biochimie 94:1982-1989(2012).
RN [8]
RP FUNCTION.
RX PubMed=24014030; DOI=10.1074/jbc.m113.501536;
RA Breyton C., Flayhan A., Gabel F., Lethier M., Durand G., Boulanger P.,
RA Chami M., Ebel C.;
RT "Assessing the conformational changes of pb5, the receptor-binding protein
RT of phage T5, upon binding to its Escherichia coli receptor FhuA.";
RL J. Biol. Chem. 288:30763-30772(2013).
CC -!- FUNCTION: Structural component of the distal part of the tail. Mediates
CC T5 binding to its host receptor, the E.coli outer membrane ferrichrome
CC transporter fhuA protein. Upon binding to host FhuA, pb5 undergoes
CC conformational changes that are probably the key to the transmission of
CC the signal through the tail to the capsid, triggering DNA release.
CC {ECO:0000269|PubMed:12051859, ECO:0000269|PubMed:22659573,
CC ECO:0000269|PubMed:24014030}.
CC -!- SUBUNIT: Monomer. Interacts with the host FhuA receptor; this
CC interaction is necessary for the entry of the viral genome into the
CC host cell, although pb5 alone is not sufficient for the conversion of
CC FhuA into an open channel. {ECO:0000269|PubMed:12051859}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:24198424}.
CC Note=Localizes at the tip of the central tail fiber.
CC {ECO:0000269|PubMed:24198424}.
CC -!- MISCELLANEOUS: Only small regions of the protein may be involved in
CC protein-protein interactions in the tail structure, the major part
CC being used in receptor binding.
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DR EMBL; M62847; AAA32559.1; -; Genomic_DNA.
DR EMBL; AY543070; AAS77196.1; -; Genomic_DNA.
DR EMBL; AY692264; AAU05292.1; -; Genomic_DNA.
DR EMBL; AY587007; AAX12083.1; -; Genomic_DNA.
DR EMBL; X75922; CAA53526.1; -; Genomic_DNA.
DR PIR; A38181; ZYBPT5.
DR RefSeq; YP_006985.1; NC_005859.1.
DR GeneID; 2777681; -.
DR KEGG; vg:2777681; -.
DR Proteomes; UP000002107; Genome.
DR Proteomes; UP000002141; Genome.
DR Proteomes; UP000002503; Genome.
DR GO; GO:0098015; C:virus tail; IEA:UniProtKB-KW.
DR GO; GO:0098670; P:entry receptor-mediated virion attachment to host cell; IEA:UniProtKB-KW.
DR GO; GO:0046813; P:receptor-mediated virion attachment to host cell; IDA:UniProtKB.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Host-virus interaction; Late protein; Reference proteome;
KW Viral attachment to host cell; Viral attachment to host entry receptor;
KW Viral tail protein; Virion; Virus entry into host cell.
FT CHAIN 1..640
FT /note="Receptor-binding protein pb5"
FT /id="PRO_0000165219"
FT CONFLICT 431
FT /note="A -> T (in Ref. 1; AAA32559)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 640 AA; 68726 MW; 9FB971D389871317 CRC64;
MSFFAGKLNN KSILSLRRGS GGDTNQHINP DSQTIFHSDM SHVIITETHS TGLRLDQGAG
DYYWSEMPSR VTQLHNNDPN RVVLTEIEFS DGSRHMLSGM SMGVGAKAYG IINPQIMSQG
GLKTQITASA DLSLDVGYFN TGTSGTIPQK LRDGTGCQHM FGAFSGRRGF ASSAMYLGGA
ALYKSAWSGS GYVVADAGTL TIPSDYVRHP GARNFGFNAI YVRGRSCNRV LYGMEGPNYT
TGGAVQGASS SGALNFTYNP SNPESPKYSV GFARADPTNY AYWESMGDPN DSANGPIGIY
SEHLGIYPSK ITWYVTNLVY NGSGYNIDGG LFNGNDIKLS PREFIIKGVN VNNTSWKFIN
FIEKNFNVGN RADFRDVGCN LSKDSPSTGI SGIATFGLPT TESNNAPSIK GGNVGGLHAN
VVSIYNFLPS ASWYVSSNPP KIGNNYGDVW SENLLPLRLL GGSGSTILSG NIVFQGNGSV
HVGTVGLDLN SSRNGAIVCT MEFIDDTWLS AGGIGCFNPT EMLSQGAEYG DSRFRIGGNT
INKKLHQILS LPAGEYVPFF TIKGTVVNAC KLQAAAYNPT PYWVSGLPGS VGQTGYYTLT
YYMRNDGNNN ISIWLDSSMS NIIGMKACLP NIKLIIQRLT
