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RBPA_MYCS2
ID   RBPA_MYCS2              Reviewed;         114 AA.
AC   A0QZ11; E9M4Y7;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2013, sequence version 2.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=RNA polymerase-binding protein RbpA {ECO:0000255|HAMAP-Rule:MF_01483};
GN   Name=rbpA {ECO:0000255|HAMAP-Rule:MF_01483};
GN   OrderedLocusNames=MSMEG_3858, MSMEI_3768;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH RNA
RP   POLYMERASE, INTERACTION WITH BETA SUBUNIT (RPOB), ANTIBIOTIC RESISTANCE,
RP   AND INDUCTION.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=19926651; DOI=10.1099/mic.0.033670-0;
RA   Dey A., Verma A.K., Chatterji D.;
RT   "Role of an RNA polymerase interacting protein, MsRbpA, from Mycobacterium
RT   smegmatis in phenotypic tolerance to rifampicin.";
RL   Microbiology 156:873-883(2010).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [5]
RP   PROTEIN SEQUENCE OF 58-73, INTERACTION WITH RNA POLYMERASE BETA SUBUNIT
RP   (RPOB), FUNCTION, AND ANTIBIOTIC RESISTANCE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=21415119; DOI=10.1099/mic.0.047480-0;
RA   Dey A., Verma A.K., Chatterji D.;
RT   "Molecular insights into the mechanism of phenotypic tolerance to
RT   rifampicin conferred on mycobacterial RNA polymerase by MsRbpA.";
RL   Microbiology 157:2056-2071(2011).
CC   -!- FUNCTION: Binds to RNA polymerase (RNAP), probably stimulating
CC       transcriptions from principal, but not alternative sigma factor
CC       promoters (By similarity). Partially restores transcription in the
CC       presence of rifampicin (Rif) in vitro; overexpression leads to an
CC       increase in the Rif tolerance in vivo, with smaller colonies. Seems to
CC       act by removing Rif from its binding site and preventing its further
CC       binding. No longer stimulates transcription in Rif-resistant RNA
CC       polymerase (with mutations in rpoB). {ECO:0000250,
CC       ECO:0000269|PubMed:19926651, ECO:0000269|PubMed:21415119}.
CC   -!- SUBUNIT: Monomer. Forms a complex with the RNAP catalytic core,
CC       specifically with the beta subunit (RpoB); its binding site may overlap
CC       with that of Rif. May bind free principal sigma factors.
CC   -!- INDUCTION: Constitutively expressed during growth, increases slightly
CC       in stationary phase (at protein level). {ECO:0000269|PubMed:19926651}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase-binding protein RbpA family.
CC       {ECO:0000255|HAMAP-Rule:MF_01483}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABK74095.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; HQ203032; ADV91011.1; -; Genomic_DNA.
DR   EMBL; CP000480; ABK74095.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP001663; AFP40226.1; -; Genomic_DNA.
DR   RefSeq; WP_003895305.1; NZ_SIJM01000005.1.
DR   RefSeq; YP_888149.1; NC_008596.1.
DR   PDB; 5TW1; X-ray; 2.76 A; J=1-114.
DR   PDB; 5VI5; X-ray; 3.20 A; J=1-114.
DR   PDB; 5VI8; X-ray; 2.76 A; J=1-114.
DR   PDB; 6CCE; X-ray; 3.05 A; J=1-114.
DR   PDB; 6CCV; X-ray; 3.05 A; J=1-114.
DR   PDB; 6DCF; X-ray; 3.45 A; J=1-114.
DR   PDB; 6VVS; X-ray; 3.11 A; J=1-114.
DR   PDB; 6VVT; X-ray; 2.90 A; J=1-114.
DR   PDB; 6VVV; X-ray; 3.20 A; J=1-114.
DR   PDB; 7P5X; EM; 3.20 A; AJ=1-114.
DR   PDBsum; 5TW1; -.
DR   PDBsum; 5VI5; -.
DR   PDBsum; 5VI8; -.
DR   PDBsum; 6CCE; -.
DR   PDBsum; 6CCV; -.
DR   PDBsum; 6DCF; -.
DR   PDBsum; 6VVS; -.
DR   PDBsum; 6VVT; -.
DR   PDBsum; 6VVV; -.
DR   PDBsum; 7P5X; -.
DR   AlphaFoldDB; A0QZ11; -.
DR   SMR; A0QZ11; -.
DR   STRING; 246196.MSMEI_3768; -.
DR   EnsemblBacteria; ABK74095; ABK74095; MSMEG_3858.
DR   EnsemblBacteria; AFP40226; AFP40226; MSMEI_3768.
DR   GeneID; 66735225; -.
DR   KEGG; msg:MSMEI_3768; -.
DR   KEGG; msm:MSMEG_3858; -.
DR   PATRIC; fig|246196.19.peg.3797; -.
DR   eggNOG; ENOG5032SI2; Bacteria.
DR   OrthoDB; 1912152at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0001000; F:bacterial-type RNA polymerase core enzyme binding; IDA:UniProtKB.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   GO; GO:0046677; P:response to antibiotic; IDA:UniProtKB.
DR   Gene3D; 2.20.28.270; -; 1.
DR   HAMAP; MF_01483; RbpA; 1.
DR   InterPro; IPR038638; RbpA_sf.
DR   InterPro; IPR025182; RNApol-bd_RbpA.
DR   Pfam; PF13397; RbpA; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic resistance; Direct protein sequencing;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..114
FT                   /note="RNA polymerase-binding protein RbpA"
FT                   /id="PRO_0000423658"
FT   STRAND          27..34
FT                   /evidence="ECO:0007829|PDB:5TW1"
FT   STRAND          39..45
FT                   /evidence="ECO:0007829|PDB:5TW1"
FT   STRAND          46..48
FT                   /evidence="ECO:0007829|PDB:6VVT"
FT   STRAND          52..55
FT                   /evidence="ECO:0007829|PDB:6VVT"
FT   STRAND          62..67
FT                   /evidence="ECO:0007829|PDB:5TW1"
FT   HELIX           81..86
FT                   /evidence="ECO:0007829|PDB:5TW1"
FT   HELIX           91..106
FT                   /evidence="ECO:0007829|PDB:5TW1"
FT   TURN            109..111
FT                   /evidence="ECO:0007829|PDB:6VVV"
SQ   SEQUENCE   114 AA;  13057 MW;  26C9F7D4313B74E5 CRC64;
     MADRVLRGSR LGAVSYETDR NHDLAPRQVA RYRTDNGEEF DVPFADDAEI PGTWLCRNGL
     EGTLIEGDVP EPKKVKPPRT HWDMLLERRS VEELEELLKE RLDLIKAKRR GTGS
 
 
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