RBPS2_CHICK
ID RBPS2_CHICK Reviewed; 200 AA.
AC Q9W6I1;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=RNA-binding protein with multiple splicing 2 {ECO:0000250|UniProtKB:Q6ZRY4};
DE AltName: Full=Heart and RRM expressed sequence {ECO:0000303|PubMed:10096065};
DE Short=Hermes {ECO:0000303|PubMed:10096065};
GN Name=RBPMS2 {ECO:0000305};
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Heart;
RX PubMed=10096065; DOI=10.1016/s0925-4773(98)00195-6;
RA Gerber W.V., Yatskievych T.A., Antin P.B., Correia K.M., Conlon R.A.,
RA Krieg P.A.;
RT "The RNA-binding protein gene, hermes, is expressed at high levels in the
RT developing heart.";
RL Mech. Dev. 80:77-86(1999).
RN [2]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=22683258; DOI=10.1053/j.gastro.2012.05.047;
RA Notarnicola C., Rouleau C., Le Guen L., Virsolvy A., Richard S., Faure S.,
RA De Santa Barbara P.;
RT "The RNA-binding protein RBPMS2 regulates development of gastrointestinal
RT smooth muscle.";
RL Gastroenterology 143:687-697(2012).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, RNA-BINDING, AND MUTAGENESIS OF
RP LEU-40.
RX PubMed=25064856; DOI=10.1093/nar/gku692;
RA Sagnol S., Yang Y., Bessin Y., Allemand F., Hapkova I., Notarnicola C.,
RA Guichou J.F., Faure S., Labesse G., de Santa Barbara P.;
RT "Homodimerization of RBPMS2 through a new RRM-interaction motif is
RT necessary to control smooth muscle plasticity.";
RL Nucleic Acids Res. 42:10173-10184(2014).
CC -!- FUNCTION: RNA-binding protein involved in the regulation of smooth
CC muscle cell differentiation and proliferation in the gastrointestinal
CC system (PubMed:22683258, PubMed:25064856). Binds NOG mRNA, the major
CC inhibitor of the bone morphogenetic protein (BMP) pathway
CC (PubMed:25064856). Mediates an increase of NOG mRNA levels, thereby
CC contributing to the negative regulation of BMP signaling pathway and
CC promoting reversible dedifferentiation and proliferation of smooth
CC muscle cells (PubMed:25064856). {ECO:0000269|PubMed:22683258,
CC ECO:0000269|PubMed:25064856}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:25064856}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q6ZRY4}.
CC -!- TISSUE SPECIFICITY: Expressed in developing heart.
CC {ECO:0000269|PubMed:10096065}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in embryonic stomach, midgut and
CC colon during the period of visceral smooth muscle cell differentiation;
CC levels decrease rapidly thereafter (PubMed:22683258). mRNA already
CC detected at stage 7-8 in the cardiogenic mesoderm, and become almost
CC undetectable in the outer curvature of the ventricular region whereas
CC remaining high in the developing atrial regions (PubMed:10096065).
CC {ECO:0000269|PubMed:10096065, ECO:0000269|PubMed:22683258}.
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DR EMBL; AF129933; AAD30273.1; -; mRNA.
DR RefSeq; NP_990200.1; NM_204869.1.
DR AlphaFoldDB; Q9W6I1; -.
DR SMR; Q9W6I1; -.
DR STRING; 9031.ENSGALP00000039532; -.
DR Ensembl; ENSGALT00000073660; ENSGALP00000053109; ENSGALG00000030988.
DR GeneID; 395678; -.
DR KEGG; gga:395678; -.
DR CTD; 348093; -.
DR VEuPathDB; HostDB:geneid_395678; -.
DR eggNOG; KOG1457; Eukaryota.
DR GeneTree; ENSGT00940000158086; -.
DR HOGENOM; CLU_099973_1_1_1; -.
DR InParanoid; Q9W6I1; -.
DR OMA; MRWFPSS; -.
DR OrthoDB; 1368991at2759; -.
DR PhylomeDB; Q9W6I1; -.
DR PRO; PR:Q9W6I1; -.
DR Proteomes; UP000000539; Chromosome 10.
DR Bgee; ENSGALG00000030988; Expressed in heart and 11 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; IDA:UniProtKB.
DR GO; GO:0008143; F:poly(A) binding; ISS:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; IPI:UniProtKB.
DR GO; GO:0003713; F:transcription coactivator activity; ISS:UniProtKB.
DR GO; GO:0048565; P:digestive tract development; IMP:UniProtKB.
DR GO; GO:0048557; P:embryonic digestive tract morphogenesis; IMP:UniProtKB.
DR GO; GO:0030514; P:negative regulation of BMP signaling pathway; IMP:UniProtKB.
DR GO; GO:0051151; P:negative regulation of smooth muscle cell differentiation; IMP:UniProtKB.
DR GO; GO:0010862; P:positive regulation of pathway-restricted SMAD protein phosphorylation; ISS:UniProtKB.
DR GO; GO:0060391; P:positive regulation of SMAD protein signal transduction; ISS:UniProtKB.
DR GO; GO:0048661; P:positive regulation of smooth muscle cell proliferation; IMP:UniProtKB.
DR CDD; cd12683; RRM_RBPMS2; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR034787; RBPMS2_RRM.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Reference proteome; RNA-binding.
FT CHAIN 1..200
FT /note="RNA-binding protein with multiple splicing 2"
FT /id="PRO_0000081795"
FT DOMAIN 22..99
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 32..42
FT /note="Important for homodimerization"
FT /evidence="ECO:0000250|UniProtKB:Q6ZRY4"
FT MUTAGEN 40
FT /note="L->A: Abolishes homodimerization and up-regulation
FT of NOG mRNA levels. Loss of function in gastrointestinal
FT smooth muscle cell differentiation and in gastrointestinal
FT smooth muscle development."
FT /evidence="ECO:0000269|PubMed:25064856"
SQ SEQUENCE 200 AA; 21856 MW; 4093B3C780BBC1DF CRC64;
MSNLNKDTEH TNGGGNVEEE VRTLFVSGLP VDIKPRELYL LFRPFKGYEG SLIKLTSKQP
VGFVTFDSRA GAEAAKNALN GIRFDPENPQ TLRLEFAKAN TKMAKSKLMA TPNPTNIHPA
LGAHFIARDP YDLTGAALIP ASPEAWAPYP LYTTELTPAI PHAAFTYPAA AAAAAALHAQ
MRWYPPSEAT QQGWKSRQFC
