RBR2_CANAL
ID RBR2_CANAL Reviewed; 168 AA.
AC Q5A6M2; A0A1D8PST4; Q5A6W7;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 2.
DT 25-MAY-2022, entry version 75.
DE RecName: Full=Repressed By RIM101 protein 2;
DE EC=3.1.1.-;
DE AltName: Full=Predicted GPI-anchored protein 21;
DE Flags: Precursor;
GN Name=RBR2; Synonyms=PGA21; OrderedLocusNames=CAALFM_CR04420CA;
GN ORFNames=CaO19.532, CaO19.8165;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP INDUCTION.
RX PubMed=12397174; DOI=10.1073/pnas.232566499;
RA Lan C.Y., Newport G., Murillo L.A., Jones T., Scherer S., Davis R.W.,
RA Agabian N.;
RT "Metabolic specialization associated with phenotypic switching in
RT Candidaalbicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:14907-14912(2002).
RN [5]
RP PREDICTION OF GPI-ANCHOR.
RX PubMed=12845604; DOI=10.1002/yea.1007;
RA De Groot P.W., Hellingwerf K.J., Klis F.M.;
RT "Genome-wide identification of fungal GPI proteins.";
RL Yeast 20:781-796(2003).
RN [6]
RP INDUCTION.
RX PubMed=15189998; DOI=10.1128/ec.3.3.776-784.2004;
RA Lotz H., Sohn K., Brunner H., Muhlschlegel F.A., Rupp S.;
RT "RBR1, a novel pH-regulated cell wall gene of Candida albicans, is
RT repressed by RIM101 and activated by NRG1.";
RL Eukaryot. Cell 3:776-784(2004).
RN [7]
RP INDUCTION.
RX PubMed=16987174; DOI=10.1111/j.1365-2958.2006.05367.x;
RA Bennett R.J., Johnson A.D.;
RT "The role of nutrient regulation and the Gpa2 protein in the mating
RT pheromone response of C. albicans.";
RL Mol. Microbiol. 62:100-119(2006).
RN [8]
RP INDUCTION.
RX PubMed=17164403; DOI=10.1074/mcp.m600210-mcp200;
RA Fernandez-Arenas E., Cabezon V., Bermejo C., Arroyo J., Nombela C.,
RA Diez-Orejas R., Gil C.;
RT "Integrated proteomics and genomics strategies bring new insight into
RT Candida albicans response upon macrophage interaction.";
RL Mol. Cell. Proteomics 6:460-478(2007).
CC -!- FUNCTION: Probable cell wall protein which may have esterase activity,
CC with a preference for esters of fatty acids from 4 to 16 carbon atoms.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}. Membrane
CC {ECO:0000305}; Lipid-anchor, GPI-anchor {ECO:0000305}.
CC -!- INDUCTION: Expression is regulated upon white-opaque switching.
CC Repressed by RIM101 and alpha pheromone, and up-regulated upon
CC interaction with macrophages. {ECO:0000269|PubMed:12397174,
CC ECO:0000269|PubMed:15189998, ECO:0000269|PubMed:16987174,
CC ECO:0000269|PubMed:17164403}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer (By
CC similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SRP1/TIP1 family. {ECO:0000305}.
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DR EMBL; CP017630; AOW31179.1; -; Genomic_DNA.
DR RefSeq; XP_717395.2; XM_712302.2.
DR AlphaFoldDB; Q5A6M2; -.
DR STRING; 237561.Q5A6M2; -.
DR GeneID; 3640983; -.
DR KEGG; cal:CAALFM_CR04420CA; -.
DR CGD; CAL0000175612; RBR2.
DR VEuPathDB; FungiDB:CR_04420C_A; -.
DR eggNOG; ENOG502RZ9R; Eukaryota.
DR HOGENOM; CLU_129392_0_0_1; -.
DR InParanoid; Q5A6M2; -.
DR OMA; ATELPWY; -.
DR OrthoDB; 1643601at2759; -.
DR PRO; PR:Q5A6M2; -.
DR Proteomes; UP000000559; Chromosome R.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR InterPro; IPR000992; SRP1_TIP1.
DR Pfam; PF00660; SRP1_TIP1; 1.
PE 1: Evidence at protein level;
KW Cell wall; Glycoprotein; GPI-anchor; Hydrolase; Lipoprotein; Membrane;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..143
FT /note="Repressed By RIM101 protein 2"
FT /id="PRO_0000424781"
FT PROPEP 144..168
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000424782"
FT REGION 111..149
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 111..146
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 143
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 83
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 168 AA; 16830 MW; 8C8A673C49957E75 CRC64;
MRFAFTTVSL SLLLSSLVAS DAASSDVQFL TALVGDYQDH KTDYIKFFAT AKDVPGDLST
LATKVLTYTD DSYTTLLNDD SLNVSNLEAY ATSLPWYSRI QADAGGKGSA SGSASGSGSA
KSTASAEKSS GSSASASSTA GGSSSKGGVS ELVAPVGAVV GALAVALM
