RBS2B_ARATH
ID RBS2B_ARATH Reviewed; 181 AA.
AC P10797; Q9FF20;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2003, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Ribulose bisphosphate carboxylase small subunit 2B, chloroplastic;
DE Short=RuBisCO small subunit 2B;
DE Flags: Precursor;
GN Name=RBCS-2B; Synonyms=ATS2B; OrderedLocusNames=At5g38420;
GN ORFNames=MXI10.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia K85;
RX AGRICOLA=IND91035191; DOI=10.1007/BF00019515;
RA Krebbers E., Seurinck J., Herdies L., Cashmore A.R., Timko M.P.;
RT "Four genes in two diverged subfamilies encode the ribulose-1,5-
RT bisphosphate carboxylase small subunit polypeptides of Arabidopsis
RT thaliana.";
RL Plant Mol. Biol. 11:745-759(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 56-100.
RC STRAIN=cv. C24; TISSUE=Flower bud;
RX PubMed=8281187; DOI=10.1046/j.1365-313x.1993.04061051.x;
RA Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M.,
RA Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D.,
RA Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M.,
RA Cooke R., Grellet F., Delseny M., Parmentier Y., de Marcillac G., Gigot C.,
RA Fleck J., Philipps G., Axelos M., Bardet C., Tremousaygue D., Lescure B.;
RT "An inventory of 1152 expressed sequence tags obtained by partial
RT sequencing of cDNAs from Arabidopsis thaliana.";
RL Plant J. 4:1051-1061(1993).
CC -!- FUNCTION: RuBisCO catalyzes two reactions: the carboxylation of D-
CC ribulose 1,5-bisphosphate, the primary event in carbon dioxide
CC fixation, as well as the oxidative fragmentation of the pentose
CC substrate. Both reactions occur simultaneously and in competition at
CC the same active site. Although the small subunit is not catalytic it is
CC essential for maximal activity. {ECO:0000255|HAMAP-Rule:MF_00860}.
CC -!- SUBUNIT: Heterohexadecamer of 8 large and 8 small subunits.
CC {ECO:0000255|HAMAP-Rule:MF_00860}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_00860}.
CC -!- MISCELLANEOUS: There are four genes coding for RBS in Arabidopsis
CC thaliana.
CC -!- MISCELLANEOUS: The basic functional RuBisCO is composed of a large
CC chain homodimer in a 'head-to-tail' conformation. In form I RuBisCO
CC this homodimer is arranged in a barrel-like tetramer with the small
CC subunits forming a tetrameric 'cap' on each end of the 'barrel'.
CC {ECO:0000255|HAMAP-Rule:MF_00860}.
CC -!- SIMILARITY: Belongs to the RuBisCO small chain family.
CC {ECO:0000255|HAMAP-Rule:MF_00860}.
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DR EMBL; X14564; CAA32701.1; -; Genomic_DNA.
DR EMBL; AB005248; BAB09354.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94314.1; -; Genomic_DNA.
DR EMBL; AY059939; AAL24421.1; -; mRNA.
DR EMBL; AY062437; AAL32515.1; -; mRNA.
DR EMBL; AY062458; AAL32536.1; -; mRNA.
DR EMBL; AY062543; AAL32621.1; -; mRNA.
DR EMBL; AY093288; AAM13287.1; -; mRNA.
DR EMBL; BT002094; AAN72105.1; -; mRNA.
DR EMBL; BT002554; AAO00914.1; -; mRNA.
DR EMBL; BT003356; AAO29974.1; -; mRNA.
DR EMBL; Z18199; CAA79132.1; -; mRNA.
DR PIR; S03718; RKMUB2.
DR RefSeq; NP_198658.1; NM_123203.5.
DR AlphaFoldDB; P10797; -.
DR SMR; P10797; -.
DR BioGRID; 19080; 30.
DR IntAct; P10797; 23.
DR STRING; 3702.AT5G38420.1; -.
DR PaxDb; P10797; -.
DR PRIDE; P10797; -.
DR EnsemblPlants; AT5G38420.1; AT5G38420.1; AT5G38420.
DR GeneID; 833829; -.
DR Gramene; AT5G38420.1; AT5G38420.1; AT5G38420.
DR KEGG; ath:AT5G38420; -.
DR Araport; AT5G38420; -.
DR TAIR; locus:2177416; AT5G38420.
DR eggNOG; ENOG502QT0M; Eukaryota.
DR HOGENOM; CLU_098114_1_0_1; -.
DR InParanoid; P10797; -.
DR OMA; WNPAIEH; -.
DR OrthoDB; 1258997at2759; -.
DR PhylomeDB; P10797; -.
DR BioCyc; MetaCyc:AT5G38420-MON; -.
DR PRO; PR:P10797; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; P10797; baseline and differential.
DR Genevisible; P10797; AT.
DR GO; GO:0048046; C:apoplast; HDA:TAIR.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR GO; GO:0022626; C:cytosolic ribosome; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0009579; C:thylakoid; HDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0019904; F:protein domain specific binding; IPI:CAFA.
DR GO; GO:0009853; P:photorespiration; IEA:UniProtKB-KW.
DR GO; GO:0019253; P:reductive pentose-phosphate cycle; IEA:UniProtKB-KW.
DR CDD; cd03527; RuBisCO_small; 1.
DR Gene3D; 3.30.190.10; -; 1.
DR HAMAP; MF_00859; RuBisCO_S_bact; 1.
DR InterPro; IPR024681; RuBisCO_ssu.
DR InterPro; IPR000894; RuBisCO_ssu_dom.
DR InterPro; IPR024680; RuBisCO_ssu_N.
DR InterPro; IPR036385; RuBisCO_ssu_sf.
DR PANTHER; PTHR31262; PTHR31262; 1.
DR Pfam; PF12338; RbcS; 1.
DR Pfam; PF00101; RuBisCO_small; 1.
DR PRINTS; PR00152; RUBISCOSMALL.
DR SMART; SM00961; RuBisCO_small; 1.
DR SUPFAM; SSF55239; SSF55239; 1.
PE 2: Evidence at transcript level;
KW Calvin cycle; Carbon dioxide fixation; Chloroplast; Photorespiration;
KW Photosynthesis; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..54
FT /note="Chloroplast"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00860"
FT CHAIN 55..181
FT /note="Ribulose bisphosphate carboxylase small subunit 2B,
FT chloroplastic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00860"
FT /id="PRO_0000031465"
FT CONFLICT 6
FT /note="F -> L (in Ref. 1; CAA32701)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 181 AA; 20350 MW; AB149EBBF84A1CC2 CRC64;
MASSMFSSTA VVTSPAQATM VAPFTGLKSS ASFPVTRKAN NDITSITSNG GRVSCMKVWP
PIGKKKFETL SYLPDLSDVE LAKEVDYLLR NKWIPCVEFE LEHGFVYREH GNTPGYYDGR
YWTMWKLPLF GCTDSAQVLK EVEECKKEYP GAFIRIIGFD NTRQVQCISF IAYKPPSFTE
A
