RBSA_BACSU
ID RBSA_BACSU Reviewed; 493 AA.
AC P36947; P96732;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Ribose import ATP-binding protein RbsA {ECO:0000255|HAMAP-Rule:MF_01716};
DE EC=7.5.2.7 {ECO:0000255|HAMAP-Rule:MF_01716};
GN Name=rbsA {ECO:0000255|HAMAP-Rule:MF_01716}; OrderedLocusNames=BSU35940;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=7921236; DOI=10.1099/13500872-140-8-1829;
RA Woodson K., Devine K.M.;
RT "Analysis of a ribose transport operon from Bacillus subtilis.";
RL Microbiology 140:1829-1838(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9353933; DOI=10.1099/00221287-143-10-3313;
RA Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V.,
RA Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G.,
RA Kunst F., Danchin A., Glaser P.;
RT "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334
RT degrees).";
RL Microbiology 143:3313-3328(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
CC -!- FUNCTION: Part of the ABC transporter complex RbsABC involved in ribose
CC import. Responsible for energy coupling to the transport system.
CC {ECO:0000255|HAMAP-Rule:MF_01716}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-ribose(out) + H2O = ADP + D-ribose(in) + H(+) +
CC phosphate; Xref=Rhea:RHEA:29903, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:47013, ChEBI:CHEBI:456216; EC=7.5.2.7;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01716};
CC -!- SUBUNIT: The complex is composed of an ATP-binding protein (RbsA), two
CC transmembrane proteins (RbsC) and a solute-binding protein (RbsB).
CC {ECO:0000255|HAMAP-Rule:MF_01716}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01716};
CC Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01716}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Ribose importer
CC (TC 3.A.1.2.1) family. {ECO:0000255|HAMAP-Rule:MF_01716}.
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DR EMBL; Z25798; CAA81051.1; -; Genomic_DNA.
DR EMBL; Z92953; CAB07463.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15611.1; -; Genomic_DNA.
DR PIR; H69689; H69689.
DR RefSeq; NP_391475.1; NC_000964.3.
DR RefSeq; WP_003244379.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; P36947; -.
DR SMR; P36947; -.
DR STRING; 224308.BSU35940; -.
DR jPOST; P36947; -.
DR PaxDb; P36947; -.
DR PRIDE; P36947; -.
DR DNASU; 936839; -.
DR EnsemblBacteria; CAB15611; CAB15611; BSU_35940.
DR GeneID; 936839; -.
DR KEGG; bsu:BSU35940; -.
DR PATRIC; fig|224308.179.peg.3891; -.
DR eggNOG; COG1129; Bacteria.
DR InParanoid; P36947; -.
DR OMA; AKREIYQ; -.
DR PhylomeDB; P36947; -.
DR BioCyc; BSUB:BSU35940-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015611; F:ABC-type D-ribose transporter activity; IEA:RHEA.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 2.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 2.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
DR PROSITE; PS51254; RBSA; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW Reference proteome; Repeat; Sugar transport; Translocase; Transport.
FT CHAIN 1..493
FT /note="Ribose import ATP-binding protein RbsA"
FT /id="PRO_0000092955"
FT DOMAIN 3..239
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01716"
FT DOMAIN 246..493
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01716"
FT BINDING 35..42
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01716"
FT CONFLICT 43
FT /note="T -> R (in Ref. 1; CAA81051)"
FT /evidence="ECO:0000305"
FT CONFLICT 327..383
FT /note="GLGFITENRKDEGLLLDTSIRENIALPNLSSFSPKGLIDHKREAEFVDLLIK
FT RLTIK -> VSALLQRIARMKGSCS (in Ref. 1; CAA81051)"
FT /evidence="ECO:0000305"
FT CONFLICT 398..400
FT /note="NQQ -> KPGK (in Ref. 1; CAA81051)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 493 AA; 54532 MW; 48AACF3315FC69D6 CRC64;
MQIEMKDIHK TFGKNQVLSG VSFQLMPGEV HALMGENGAG KSTLMNILTG LHKADKGQIS
INGNETYFSN PKEAEQHGIA FIHQELNIWP EMTVLENLFI GKEISSKLGV LQTRKMKALA
KEQFDKLSVS LSLDQEAGEC SVGQQQMIEI AKALMTNAEV IIMDEPTAAL TEREISKLFE
VITALKKNGV SIVYISHRME EIFAICDRIT IMRDGKTVDT TNISETDFDE VVKKMVGREL
TERYPKRTPS LGDKVFEVKN ASVKGSFEDV SFYVRSGEIV GVSGLMGAGR TEMMRALFGV
DRLDTGEIWI AGKKTAIKNP QEAVKKGLGF ITENRKDEGL LLDTSIRENI ALPNLSSFSP
KGLIDHKREA EFVDLLIKRL TIKTASPETH ARHLSGGNQQ KVVIAKWIGI GPKVLILDEP
TRGVDVGAKR EIYTLMNELT ERGVAIIMVS SELPEILGMS DRIIVVHEGR ISGEIHAREA
TQERIMTLAT GGR
