ID   RBTN2_DANRE             Reviewed;         159 AA.
AC   Q9PTJ3; Q567L0;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Rhombotin-2;
DE   AltName: Full=LIM domain only protein 2;
DE            Short=LMO-2;
DE            Short=zlmo2;
GN   Name=lmo2 {ECO:0000312|ZFIN:ZDB-GENE-980526-419}; ORFNames=si:dkey-10o6.5;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAF02779.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=9630750; DOI=10.1006/dbio.1998.8887;
RA   Thompson M.A., Ransom D.G., Pratt S.J., MacLennan H., Kieran M.W.,
RA   Detrich H.W. III, Vail B., Huber T.L., Paw B., Brownlie A.J., Oates A.C.,
RA   Fritz A., Gates M.A., Amores A., Bahary N., Talbot W.S., Her H.,
RA   Beier D.R., Postlethwait J.H., Zon L.I.;
RT   "The cloche and spadetail genes differentially affect hematopoiesis and
RT   vasculogenesis.";
RL   Dev. Biol. 197:248-269(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [3] {ECO:0000312|EMBL:CAN88424.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Larva {ECO:0000312|EMBL:AAH93136.1};
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4] {ECO:0000305}
RP   FUNCTION.
RX   PubMed=14602685; DOI=10.1242/dev.00875;
RA   Gering M., Yamada Y., Rabbitts T.H., Patient R.K.;
RT   "Lmo2 and Scl/Tal1 convert non-axial mesoderm into haemangioblasts which
RT   differentiate into endothelial cells in the absence of Gata1.";
RL   Development 130:6187-6199(2003).
RN   [5] {ECO:0000305}
RP   TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=15893977; DOI=10.1016/j.ydbio.2005.01.034;
RA   Zhu H., Traver D., Davidson A.J., Dibiase A., Thisse C., Thisse B.,
RA   Nimer S., Zon L.I.;
RT   "Regulation of the lmo2 promoter during hematopoietic and vascular
RT   development in zebrafish.";
RL   Dev. Biol. 281:256-269(2005).
RN   [6] {ECO:0000305}
RP   FUNCTION.
RX   PubMed=17090656; DOI=10.1182/blood-2006-02-003087;
RA   Patterson L.J., Gering M., Eckfeldt C.E., Green A.R., Verfaillie C.M.,
RA   Ekker S.C., Patient R.K.;
RT   "The transcription factors Scl and Lmo2 act together during development of
RT   the hemangioblast in zebrafish.";
RL   Blood 109:2389-2398(2007).
CC   -!- FUNCTION: Transcription factor that acts synergistically with tal1/scl
CC       in primitive and definitive hematopoiesis, and in endothelial
CC       development. Specifies mesodermal precursors to a hemangioblast cell
CC       fate. Hemangioblasts are bipotential precursors of blood and
CC       endothelium, and in the absence of hematopoietic induction cues such as
CC       gata1, tal1/scl-lmo2-induced haemangioblasts differentiate into
CC       endothelial cells. {ECO:0000269|PubMed:14602685,
CC       ECO:0000269|PubMed:17090656}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P25801}.
CC   -!- TISSUE SPECIFICITY: During embryogenesis, expressed in hematopoietic
CC       and endothelial precursors, including the two stripes of posterior and
CC       anterior lateral plate mesoderm at the 1-somite stage that give rise to
CC       the primitive blood. {ECO:0000269|PubMed:15893977,
CC       ECO:0000269|PubMed:9630750}.
CC   -!- INDUCTION: By ets transcription factors including ets1.
CC       {ECO:0000269|PubMed:15893977}.
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DR   EMBL; AF191560; AAF02779.1; -; mRNA.
DR   EMBL; CT025900; CAN88424.1; -; Genomic_DNA.
DR   EMBL; BC093136; AAH93136.1; -; mRNA.
DR   RefSeq; NP_571186.1; NM_131111.1.
DR   AlphaFoldDB; Q9PTJ3; -.
DR   SMR; Q9PTJ3; -.
DR   STRING; 7955.ENSDARP00000115969; -.
DR   PaxDb; Q9PTJ3; -.
DR   Ensembl; ENSDART00000134247; ENSDARP00000115969; ENSDARG00000095019.
DR   GeneID; 30332; -.
DR   KEGG; dre:30332; -.
DR   CTD; 4005; -.
DR   ZFIN; ZDB-GENE-980526-419; lmo2.
DR   eggNOG; KOG0490; Eukaryota.
DR   GeneTree; ENSGT00940000160199; -.
DR   HOGENOM; CLU_001357_3_1_1; -.
DR   InParanoid; Q9PTJ3; -.
DR   OMA; TTSSECR; -.
DR   OrthoDB; 1307952at2759; -.
DR   PhylomeDB; Q9PTJ3; -.
DR   TreeFam; TF351071; -.
DR   Reactome; R-DRE-8939236; RUNX1 regulates transcription of genes involved in differentiation of HSCs.
DR   PRO; PR:Q9PTJ3; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 18.
DR   Bgee; ENSDARG00000095019; Expressed in spleen and 47 other tissues.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0032991; C:protein-containing complex; ISS:UniProtKB.
DR   GO; GO:0140297; F:DNA-binding transcription factor binding; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR   GO; GO:0001568; P:blood vessel development; IMP:ZFIN.
DR   GO; GO:0043534; P:blood vessel endothelial cell migration; IMP:ZFIN.
DR   GO; GO:0035162; P:embryonic hemopoiesis; IMP:ZFIN.
DR   GO; GO:0001935; P:endothelial cell proliferation; IMP:ZFIN.
DR   GO; GO:0048821; P:erythrocyte development; IMP:ZFIN.
DR   GO; GO:0030218; P:erythrocyte differentiation; IGI:ZFIN.
DR   GO; GO:0031101; P:fin regeneration; IMP:ZFIN.
DR   GO; GO:0030097; P:hemopoiesis; IMP:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0061304; P:retinal blood vessel morphogenesis; IMP:ZFIN.
DR   InterPro; IPR001781; Znf_LIM.
DR   Pfam; PF00412; LIM; 2.
DR   SMART; SM00132; LIM; 2.
DR   PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR   PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE   2: Evidence at transcript level;
KW   Developmental protein; LIM domain; Metal-binding; Nucleus;
KW   Reference proteome; Repeat; Zinc.
FT   CHAIN           1..159
FT                   /note="Rhombotin-2"
FT                   /id="PRO_0000318111"
FT   DOMAIN          28..90
FT                   /note="LIM zinc-binding 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DOMAIN          92..154
FT                   /note="LIM zinc-binding 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   CONFLICT        131
FT                   /note="V -> A (in Ref. 3; AAH93136)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   159 AA;  18455 MW;  B6CA00D7126157AE CRC64;
     MASTIERKTL EANEEPVDEV LQMPPSLLTC GGCQQSIGDR FFLKAIEQYW HEDCLSCDLC
     GCRLGEVGRR LYYKLGRKLC RRDYLRLFGQ DGLCASCEKR IRAFEMTMRV RDKVYHLECF
     KCAACQKHFC VGDRYLLINS DIVCEQDIFE WTKLNGSIV