ID   RBTN2_XENLA             Reviewed;         158 AA.
AC   Q90XH3;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Rhombotin-2;
DE   AltName: Full=LIM domain only protein 2;
DE            Short=LMO-2;
DE            Short=Lmo2-A;
DE            Short=xLMO-2;
GN   Name=lmo2 {ECO:0000303|PubMed:17942750}; Synonyms=lmo2-a;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAK54614.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP   STAGE, AND INDUCTION.
RC   TISSUE=Spleen {ECO:0000269|PubMed:11493549};
RX   PubMed=11493549; DOI=10.1242/dev.128.12.2301;
RA   Mead P.E., Deconinck A.E., Huber T.L., Orkin S.H., Zon L.I.;
RT   "Primitive erythropoiesis in the Xenopus embryo: the synergistic role of
RT   LMO-2, SCL and GATA-binding proteins.";
RL   Development 128:2301-2308(2001).
RN   [2] {ECO:0000312|EMBL:AAH97502.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Spleen {ECO:0000312|EMBL:AAH97502.1};
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000305}
RP   INDUCTION.
RX   PubMed=17942750; DOI=10.1182/blood-2007-03-081323;
RA   Walmsley M., Cleaver D., Patient R.K.;
RT   "Fibroblast growth factor controls the timing of Scl, Lmo2, and Runx1
RT   expression during embryonic blood development.";
RL   Blood 111:1157-1166(2008).
CC   -!- FUNCTION: Transcription factor that acts synergistically with tal1/scl
CC       and gata1 to specify embryonic dorsal mesoderm to a hematopoietic fate.
CC       Induces globin gene expression together with fgf.
CC       {ECO:0000269|PubMed:11493549}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P25801}.
CC   -!- TISSUE SPECIFICITY: Expression becomes restricted to the ventral blood
CC       island (VBI) as the embryo develops. In late neurula and early tailbud
CC       embryos, also expressed in the dorsal lateral plate (DLP), the site of
CC       definitive hematopoiesis in the tadpole. Expression in the DLP
CC       diminishes during tailbud stages. Expressed in circulating blood cells
CC       of tadpoles. Also expressed in non-hematopoietic sites, including the
CC       tailbud region and the central nervous system of early neurula embryos.
CC       {ECO:0000269|PubMed:11493549}.
CC   -!- DEVELOPMENTAL STAGE: Zygotic expression begins after gastrulation at
CC       stage 12.5. {ECO:0000269|PubMed:11493549}.
CC   -!- INDUCTION: By bmp-signaling. Inhibition by fgf regulates the timing of
CC       hematopoiesis. {ECO:0000269|PubMed:11493549,
CC       ECO:0000269|PubMed:17942750}.
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DR   EMBL; AF374473; AAK54614.1; -; mRNA.
DR   EMBL; BC097502; AAH97502.1; -; mRNA.
DR   RefSeq; NP_001081112.1; NM_001087643.1.
DR   AlphaFoldDB; Q90XH3; -.
DR   SMR; Q90XH3; -.
DR   DNASU; 394388; -.
DR   GeneID; 394388; -.
DR   KEGG; xla:394388; -.
DR   CTD; 394388; -.
DR   Xenbase; XB-GENE-6254426; lmo2.S.
DR   OMA; NIVCEDH; -.
DR   OrthoDB; 1307952at2759; -.
DR   Proteomes; UP000186698; Chromosome 4S.
DR   Bgee; 394388; Expressed in lung and 15 other tissues.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0032991; C:protein-containing complex; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030218; P:erythrocyte differentiation; IGI:UniProtKB.
DR   InterPro; IPR001781; Znf_LIM.
DR   Pfam; PF00412; LIM; 2.
DR   SMART; SM00132; LIM; 2.
DR   PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR   PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE   2: Evidence at transcript level;
KW   Developmental protein; LIM domain; Metal-binding; Nucleus;
KW   Reference proteome; Repeat; Zinc.
FT   CHAIN           1..158
FT                   /note="Rhombotin-2"
FT                   /id="PRO_0000318112"
FT   DOMAIN          28..90
FT                   /note="LIM zinc-binding 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DOMAIN          92..154
FT                   /note="LIM zinc-binding 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
SQ   SEQUENCE   158 AA;  18350 MW;  4C8BBFB69BC28853 CRC64;
     MSSAIERKSL DPADEPVDEV LQIPPSLLTC GGCQQSIGDR YFLKAIDQYW HEDCLSCDLC
     GCRLGEVGRR LYYKLGRKLC RRDYLRLFGQ DGLCASCDNR IRAYEMTMRV KDKVYHLECF
     KCAACQKHFC VGDRYLLINS DIVCEQDIYE WTKLSEMM