RBY1F_HUMAN
ID RBY1F_HUMAN Reviewed; 496 AA.
AC Q15415; B2R916;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=RNA-binding motif protein, Y chromosome, family 1 member F/J;
DE AltName: Full=Y chromosome RNA recognition motif 2;
GN Name=RBMY1F; Synonyms=YRRM2;
GN and
GN Name=RBMY1J;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=8269511; DOI=10.1016/0092-8674(93)90616-x;
RA Ma K., Inglis J.D., Sharkey A., Bickmore W.A., Hill R.E., Prosser E.J.,
RA Speedson R.M., Thomson E.J., Jobling M.;
RT "A Y chromosome gene family with RNA-binding protein homology: candidates
RT for the azoospermia factor AZF controlling human spermatogenesis.";
RL Cell 75:1287-1295(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12815422; DOI=10.1038/nature01722;
RA Skaletsky H., Kuroda-Kawaguchi T., Minx P.J., Cordum H.S., Hillier L.W.,
RA Brown L.G., Repping S., Pyntikova T., Ali J., Bieri T., Chinwalla A.,
RA Delehaunty A., Delehaunty K., Du H., Fewell G., Fulton L., Fulton R.,
RA Graves T.A., Hou S.-F., Latrielle P., Leonard S., Mardis E., Maupin R.,
RA McPherson J., Miner T., Nash W., Nguyen C., Ozersky P., Pepin K., Rock S.,
RA Rohlfing T., Scott K., Schultz B., Strong C., Tin-Wollam A., Yang S.-P.,
RA Waterston R.H., Wilson R.K., Rozen S., Page D.C.;
RT "The male-specific region of the human Y chromosome is a mosaic of discrete
RT sequence classes.";
RL Nature 423:825-837(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: RNA-binding protein which may be involved in spermatogenesis.
CC Required for sperm development, possibly by participating in pre-mRNA
CC splicing in the testis. {ECO:0000269|PubMed:8269511}.
CC -!- SUBUNIT: Interacts with splicing factor proteins SFRS3/SRP20,
CC TRA2B/SFRS10, KHDRBS1/SAM68 and KHDRBS3.
CC -!- INTERACTION:
CC Q15415; Q8WTP8: AEN; NbExp=3; IntAct=EBI-8642021, EBI-8637627;
CC Q15415; Q9NRW3: APOBEC3C; NbExp=6; IntAct=EBI-8642021, EBI-1044593;
CC Q15415; Q14781: CBX2; NbExp=3; IntAct=EBI-8642021, EBI-745934;
CC Q15415; Q14781-2: CBX2; NbExp=3; IntAct=EBI-8642021, EBI-11974585;
CC Q15415; Q14011: CIRBP; NbExp=3; IntAct=EBI-8642021, EBI-538850;
CC Q15415; P49761: CLK3; NbExp=3; IntAct=EBI-8642021, EBI-745579;
CC Q15415; Q8NE01: CNNM3; NbExp=3; IntAct=EBI-8642021, EBI-741032;
CC Q15415; Q7L775: EPM2AIP1; NbExp=3; IntAct=EBI-8642021, EBI-6255981;
CC Q15415; Q03014: HHEX; NbExp=3; IntAct=EBI-8642021, EBI-747421;
CC Q15415; P61978: HNRNPK; NbExp=4; IntAct=EBI-8642021, EBI-304185;
CC Q15415; P61978-2: HNRNPK; NbExp=3; IntAct=EBI-8642021, EBI-7060731;
CC Q15415; Q8TBB1: LNX1; NbExp=3; IntAct=EBI-8642021, EBI-739832;
CC Q15415; Q9NQX5: NPDC1; NbExp=3; IntAct=EBI-8642021, EBI-748927;
CC Q15415; Q9Y5E9: PCDHB14; NbExp=6; IntAct=EBI-8642021, EBI-10329013;
CC Q15415; O75864: PPP1R37; NbExp=3; IntAct=EBI-8642021, EBI-5235692;
CC Q15415; Q8WWY3: PRPF31; NbExp=9; IntAct=EBI-8642021, EBI-1567797;
CC Q15415; P79522: PRR3; NbExp=7; IntAct=EBI-8642021, EBI-2803328;
CC Q15415; Q9BTL3: RAMAC; NbExp=3; IntAct=EBI-8642021, EBI-744023;
CC Q15415; Q9Y272: RASD1; NbExp=6; IntAct=EBI-8642021, EBI-740818;
CC Q15415; P98175: RBM10; NbExp=3; IntAct=EBI-8642021, EBI-721525;
CC Q15415; P98179: RBM3; NbExp=6; IntAct=EBI-8642021, EBI-2949699;
CC Q15415; P38159: RBMX; NbExp=6; IntAct=EBI-8642021, EBI-743526;
CC Q15415; P0DJD3-2: RBMY1A1; NbExp=3; IntAct=EBI-8642021, EBI-11994018;
CC Q15415; A6NEQ0: RBMY1E; NbExp=3; IntAct=EBI-8642021, EBI-11958200;
CC Q15415; Q15415: RBMY1J; NbExp=4; IntAct=EBI-8642021, EBI-8642021;
CC Q15415; Q15287: RNPS1; NbExp=3; IntAct=EBI-8642021, EBI-395959;
CC Q15415; P09012: SNRPA; NbExp=3; IntAct=EBI-8642021, EBI-607085;
CC Q15415; Q9H0A9-2: SPATC1L; NbExp=3; IntAct=EBI-8642021, EBI-11995806;
CC Q15415; P62995: TRA2B; NbExp=3; IntAct=EBI-8642021, EBI-725485;
CC Q15415; P26368-2: U2AF2; NbExp=3; IntAct=EBI-8642021, EBI-11097439;
CC Q15415; Q96MU7: YTHDC1; NbExp=6; IntAct=EBI-8642021, EBI-2849854;
CC Q15415; Q96PQ6: ZNF317; NbExp=5; IntAct=EBI-8642021, EBI-1210473;
CC Q15415; Q66K41: ZNF385C; NbExp=3; IntAct=EBI-8642021, EBI-8651919;
CC Q15415; Q9H9D4: ZNF408; NbExp=3; IntAct=EBI-8642021, EBI-347633;
CC Q15415; Q3KQV3: ZNF792; NbExp=3; IntAct=EBI-8642021, EBI-10240849;
CC Q15415; Q96EG3: ZNF837; NbExp=3; IntAct=EBI-8642021, EBI-11962574;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q15415-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q15415-2; Sequence=VSP_030217, VSP_030218;
CC -!- TISSUE SPECIFICITY: Testis-specific. {ECO:0000269|PubMed:8269511}.
CC -!- DEVELOPMENTAL STAGE: Expressed in all of the transcriptionally active
CC stages of germ cell development from spermatogonia through
CC spermatocytes to round spermatids.
CC -!- MISCELLANEOUS: The RBMY1 proteins are encoded by repeated regions of
CC the Y chromosome, mostly within the AZFb region. The exact number of
CC functional copies is unclear and may vary between individuals, and some
CC of them may represent pseudogenes. The proteins are very similar, which
CC makes the characterization of each protein difficult. Thus, most
CC experiments do not discriminate between the different members. One can
CC therefore suppose that reported interactions with a RBMY1 protein
CC involve all the proteins.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X76060; CAA53660.1; -; mRNA.
DR EMBL; AK313597; BAG36363.1; -; mRNA.
DR EMBL; AC023342; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC007320; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC030018; AAH30018.1; -; mRNA.
DR EMBL; BC152392; AAI52393.1; -; mRNA.
DR CCDS; CCDS35483.1; -. [Q15415-1]
DR CCDS; CCDS35484.1; -. [Q15415-1]
DR PIR; B49418; B49418.
DR RefSeq; NP_001006117.2; NM_001006117.3. [Q15415-1]
DR RefSeq; NP_001290339.1; NM_001303410.1. [Q15415-2]
DR RefSeq; NP_689798.1; NM_152585.2. [Q15415-1]
DR AlphaFoldDB; Q15415; -.
DR BioGRID; 127736; 39.
DR BioGRID; 132082; 29.
DR IntAct; Q15415; 35.
DR MINT; Q15415; -.
DR iPTMnet; Q15415; -.
DR PhosphoSitePlus; Q15415; -.
DR BioMuta; RBMY1J; -.
DR DMDM; 166199747; -.
DR jPOST; Q15415; -.
DR MassIVE; Q15415; -.
DR PaxDb; Q15415; -.
DR PeptideAtlas; Q15415; -.
DR PRIDE; Q15415; -.
DR ProteomicsDB; 60580; -. [Q15415-1]
DR ProteomicsDB; 60581; -. [Q15415-2]
DR Antibodypedia; 21887; 86 antibodies from 13 providers.
DR Antibodypedia; 69611; 3 antibodies from 2 providers.
DR DNASU; 159163; -.
DR Ensembl; ENST00000250831.5; ENSP00000250831.5; ENSG00000226941.8. [Q15415-1]
DR Ensembl; ENST00000303766.12; ENSP00000307155.7; ENSG00000169800.14. [Q15415-1]
DR GeneID; 159163; -.
DR GeneID; 378951; -.
DR KEGG; hsa:159163; -.
DR KEGG; hsa:378951; -.
DR MANE-Select; ENST00000250831.6; ENSP00000250831.5; NM_001006117.4; NP_001006117.2.
DR MANE-Select; ENST00000303766.12; ENSP00000307155.7; NM_152585.3; NP_689798.1.
DR UCSC; uc004fva.4; human. [Q15415-1]
DR CTD; 159163; -.
DR CTD; 378951; -.
DR GeneCards; RBMY1F; -.
DR GeneCards; RBMY1J; -.
DR HGNC; HGNC:23974; RBMY1F.
DR HGNC; HGNC:23917; RBMY1J.
DR HPA; ENSG00000169800; Tissue enriched (testis).
DR HPA; ENSG00000226941; Tissue enriched (testis).
DR MIM; 400006; gene.
DR neXtProt; NX_Q15415; -.
DR PharmGKB; PA134946298; -.
DR VEuPathDB; HostDB:ENSG00000169800; -.
DR VEuPathDB; HostDB:ENSG00000226941; -.
DR GeneTree; ENSGT00940000163524; -.
DR HOGENOM; CLU_042286_0_0_1; -.
DR InParanoid; Q15415; -.
DR OMA; FQSDCRR; -.
DR OrthoDB; 1248417at2759; -.
DR PhylomeDB; Q15415; -.
DR TreeFam; TF331833; -.
DR PathwayCommons; Q15415; -.
DR SignaLink; Q15415; -.
DR BioGRID-ORCS; 159163; 12 hits in 236 CRISPR screens.
DR BioGRID-ORCS; 378951; 13 hits in 236 CRISPR screens.
DR Pharos; Q15415; Tdark.
DR PRO; PR:Q15415; -.
DR Proteomes; UP000005640; Chromosome Y.
DR RNAct; Q15415; protein.
DR Bgee; ENSG00000169800; Expressed in right testis and 9 other tissues.
DR ExpressionAtlas; Q15415; baseline.
DR Genevisible; Q15415; HS.
DR GO; GO:0016607; C:nuclear speck; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0000380; P:alternative mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR012604; RBM1CTR.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF08081; RBM1CTR; 1.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome; RNA-binding.
FT CHAIN 1..496
FT /note="RNA-binding motif protein, Y chromosome, family 1
FT member F/J"
FT /id="PRO_0000314164"
FT DOMAIN 8..85
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 81..345
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 452..496
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 93..115
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 169..187
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 242..345
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 397..419
FT /note="YGTSHGAPPARGPRMSYGGSTCH -> DLSWCTTCKRASDVLWWKHLPRI
FT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:8269511"
FT /id="VSP_030217"
FT VAR_SEQ 420..496
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:8269511"
FT /id="VSP_030218"
FT CONFLICT 7
FT /note="P -> A (in Ref. 1; CAA53660)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 496 AA; 55728 MW; 66AC50828D44E9A8 CRC64;
MVEADHPGKL FIGGLNRETN EKMLKAVFGK HGPISEVLLI KDRTSKSRGF AFITFENPAD
AKNAAKDMNG TSLHGKAIKV EQAKKPSFQS GGRRRPPASS RNRSPSGSLR SARGSSGGTR
GWLPSHEGHL DDGGYTPDLK MSYSRGLIPV KRGPSSRSGG PPPKKSAPSA VARSNSWMGS
QGPMSQRREN YGVPPRRATI SSWRNDRMST RHDGYATNDG NHPSCQETRD YAPPSRGYAY
RDNGHSNRDE HSSRGYRNHR SSRETRDYAP PSRGHAYRDY GHSRRDESYS RGYRNHRSSR
ETREYAPPSR GHGYRDYGHS RRHESYSRGY RNHPSSRETR DYAPPHRDYA YRDYGHSSWD
EHSSRGYSYH DGYGEALGRD HSEHLSGSSY RDALQRYGTS HGAPPARGPR MSYGGSTCHA
YSNTRDRYGR SWESYSSCGD FHYCDREHVC RKDQRNPPSL GRVLPDPREA YGSSSYVASI
VDGGESRSEK GDSSRY
