ID   RCA2_TOBAC              Reviewed;         439 AA.
AC   Q40565; Q40566;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic;
DE            Short=RA 2;
DE            Short=RuBisCO activase 2;
DE   Flags: Precursor;
GN   Name=RCA;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. SR1; TISSUE=Leaf;
RX   PubMed=8108517; DOI=10.1104/pp.102.2.683;
RA   Qian J., Rodermel S.;
RT   "Ribulose-1,5-bisphosphate carboxylase/oxygenase activase cDNAs from
RT   Nicotiana tabacum.";
RL   Plant Physiol. 102:683-684(1993).
CC   -!- FUNCTION: Activation of RuBisCO (ribulose-1,5-bisphosphate
CC       carboxylase/oxygenase; EC 4.1.1.39) involves the ATP-dependent
CC       carboxylation of the epsilon-amino group of lysine leading to a
CC       carbamate structure.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma.
CC   -!- SIMILARITY: Belongs to the RuBisCO activase family. {ECO:0000305}.
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DR   EMBL; Z14980; CAA78703.1; -; mRNA.
DR   EMBL; Z14981; CAA78704.1; -; mRNA.
DR   PIR; S25483; S25483.
DR   RefSeq; NP_001312578.1; NM_001325649.1.
DR   AlphaFoldDB; Q40565; -.
DR   SMR; Q40565; -.
DR   STRING; 4097.Q40565; -.
DR   PRIDE; Q40565; -.
DR   GeneID; 107798594; -.
DR   KEGG; nta:107798594; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0046863; F:ribulose-1,5-bisphosphate carboxylase/oxygenase activator activity; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR044960; RCA-like.
DR   PANTHER; PTHR32429; PTHR32429; 1.
DR   Pfam; PF00004; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Chloroplast; Nucleotide-binding; Plastid; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..58
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           59..439
FT                   /note="Ribulose bisphosphate carboxylase/oxygenase activase
FT                   2, chloroplastic"
FT                   /id="PRO_0000030243"
FT   BINDING         169..176
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   VARIANT         211
FT                   /note="R -> S (in clone PTA1.1)"
FT   VARIANT         226
FT                   /note="I -> M (in clone PTA1.1)"
FT   VARIANT         335
FT                   /note="I -> V (in clone PTA1.1)"
SQ   SEQUENCE   439 AA;  48343 MW;  B1E7378DDD7B1B31 CRC64;
     MATSVSTIGA ANKAPLSLNN SVAGTSVPST AFFGKTLKKV YGKGVSSPKV TNRSLRIAAE
     EKDADPKKQT YSDRWKGLVQ DFSDDQQDIA RGKGMVDSLF QAPTGTGTHH AVLQSYEYVS
     QGLRQYNMDN TLDGFYIAPS FMDKLVVHIT KNFLKLPNIK VPLILGVWGG KGQGKSFQCE
     LVFRKMGINP IMMSAGELES GNAGEPAKLI RQRYREAAEI IRKGNICCLF INDLDAGAGR
     MGGTTQYTVN NQMVNATLMN IADNPTNVQL PGMYNKQENA RVPIIVTGND FSTLYAPLIR
     DGRMEKFYWA PTREDRIGVC KGIFRTDNVP EEAVIKIVDT FPGQSIDFFG ALRARVYDDE
     VRKWVSGTGI EAIGDKLLNS FDGPPTFEQP KMTVEKLLEY GNMLVQEQEN VKRVQLAETY
     LKEAALGDAN ADAINTGNF