ID   RCAA_HORVU              Reviewed;         464 AA.
AC   Q40073; Q40071; Q40072; Q40074;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Ribulose bisphosphate carboxylase/oxygenase activase A, chloroplastic;
DE            Short=RA A;
DE            Short=RuBisCO activase A;
DE   Flags: Precursor;
GN   Name=RCAA;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS LONG AND SHORT).
RC   STRAIN=cv. Glen, and cv. Sundance; TISSUE=Leaf;
RX   PubMed=2002016; DOI=10.1016/s0021-9258(19)67702-1;
RA   Rundle S.J., Zielinski R.E.;
RT   "Organization and expression of two tandemly oriented genes encoding
RT   ribulosebisphosphate carboxylase/oxygenase activase in barley.";
RL   J. Biol. Chem. 266:4677-4685(1991).
CC   -!- FUNCTION: Activation of RuBisCO (ribulose-1,5-bisphosphate
CC       carboxylase/oxygenase; EC 4.1.1.39) involves the ATP-dependent
CC       carboxylation of the epsilon-amino group of lysine leading to a
CC       carbamate structure.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=Long; Synonyms=RCAA1;
CC         IsoId=Q40073-1; Sequence=Displayed;
CC       Name=Short; Synonyms=RCAA2;
CC         IsoId=Q40073-2; Sequence=VSP_005540;
CC   -!- SIMILARITY: Belongs to the RuBisCO activase family. {ECO:0000305}.
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DR   EMBL; M55449; AAA63164.1; -; Genomic_DNA.
DR   EMBL; M55449; AAA63163.1; -; Genomic_DNA.
DR   EMBL; M55446; AAA62701.1; -; mRNA.
DR   EMBL; M55447; AAA62702.1; -; mRNA.
DR   PIR; T06176; T06176.
DR   AlphaFoldDB; Q40073; -.
DR   SMR; Q40073; -.
DR   PRIDE; Q40073; -.
DR   ExpressionAtlas; Q40073; baseline and differential.
DR   GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR044960; RCA-like.
DR   PANTHER; PTHR32429; PTHR32429; 1.
DR   Pfam; PF00004; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; ATP-binding; Chloroplast; Nucleotide-binding;
KW   Plastid; Transit peptide.
FT   TRANSIT         1..48
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           49..464
FT                   /note="Ribulose bisphosphate carboxylase/oxygenase activase
FT                   A, chloroplastic"
FT                   /id="PRO_0000030231"
FT   BINDING         155..162
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         428..464
FT                   /note="Missing (in isoform Short)"
FT                   /evidence="ECO:0000303|PubMed:2002016"
FT                   /id="VSP_005540"
FT   VARIANT         66
FT                   /note="Y -> T (in strain: cv. Glen)"
FT   VARIANT         92
FT                   /note="H -> D (in strain: cv. Glen)"
FT   VARIANT         316
FT                   /note="S -> C (in strain: cv. Glen)"
SQ   SEQUENCE   464 AA;  51074 MW;  066CEFC2C14AE44B CRC64;
     MAAAFSSTVG APASTPTNFL GKKLKKQVTS AVNYHGKSSK ANRFTVMAAE NIDEKRNTDK
     WKGLAYDISD DQQDITRGKG IVDSLFQAPT GHGTHEAVLS SYEYVSQGLR KYDFDNTMGG
     FYIAPAFMDK LVVHLSKNFM TLPNIKIPLI LGIWGGKGQG KSFQCELVFA KMGINPIMMS
     AGELESGNAG EPAKLIRQRY REAADMIKKG KMCCLFINDL DAGAGRMGGT TQYTVNNQMV
     NATLMNIADA PTNVQLPGMY NKRENPRVPI VVTGNDFSTL YAPLIRDGRM EKFYWAPTRD
     DRIGVCKGIF QTDNVSDESV VKIVDTFPGQ SIDFFGALRA RVYDDEVRKW VGSTGIENIG
     KRLVNSRDGP VTFEQPKMTV EKLLEYGHML VQEQDNVKRV QLADTYMSQA ALGDANQDAM
     KTGSFYGKGA QQGTLPVPEG CTDQNAKNYD PTARSDDGSC LYTF