RCAB_HORVU
ID RCAB_HORVU Reviewed; 425 AA.
AC Q42450;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Ribulose bisphosphate carboxylase/oxygenase activase B, chloroplastic;
DE Short=RA B;
DE Short=RuBisCO activase B;
DE Flags: Precursor;
GN Name=RCAB;
OS Hordeum vulgare (Barley).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX NCBI_TaxID=4513;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Sundance; TISSUE=Leaf;
RX PubMed=2002016; DOI=10.1016/s0021-9258(19)67702-1;
RA Rundle S.J., Zielinski R.E.;
RT "Organization and expression of two tandemly oriented genes encoding
RT ribulosebisphosphate carboxylase/oxygenase activase in barley.";
RL J. Biol. Chem. 266:4677-4685(1991).
CC -!- FUNCTION: Activation of RuBisCO (ribulose-1,5-bisphosphate
CC carboxylase/oxygenase; EC 4.1.1.39) involves the ATP-dependent
CC carboxylation of the epsilon-amino group of lysine leading to a
CC carbamate structure.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma.
CC -!- SIMILARITY: Belongs to the RuBisCO activase family. {ECO:0000305}.
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DR EMBL; M55449; AAA63162.1; -; Genomic_DNA.
DR EMBL; M55448; AAA62703.1; -; mRNA.
DR PIR; A23703; A23703.
DR AlphaFoldDB; Q42450; -.
DR SMR; Q42450; -.
DR PRIDE; Q42450; -.
DR ExpressionAtlas; Q42450; baseline and differential.
DR GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR044960; RCA-like.
DR PANTHER; PTHR32429; PTHR32429; 1.
DR Pfam; PF00004; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chloroplast; Nucleotide-binding; Plastid; Transit peptide.
FT TRANSIT 1..43
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 44..425
FT /note="Ribulose bisphosphate carboxylase/oxygenase activase
FT B, chloroplastic"
FT /id="PRO_0000030232"
FT BINDING 153..160
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 425 AA; 47228 MW; 1C1F1AF531550CBD CRC64;
MASAFSSTVG APASTPTIFL GKKVKNYYHG GNKMKSRVVR VMAAKKELDQ GKQTDADRWK
GLAYDISDDQ QDITRGKGIV DSLFQAPMGD GTHEAILSSY EYISQGLRKY DFDNTMDGLY
IAPAFMDKLI VHLAKNFMTL PNIKVPLILG IWGGKGQGKS FQCELVFAKM GINPIMMSAG
ELESGNGEPA KLIRQRYREA ADIINKGKMC CLFINDLDAG AGRMGGTTQY TVNNQMVNAT
LMNIADAPTN VQLPGMYNKE ENPRVPIIVT GNDFSTLYAP LIRDGRMEKF YWAPTREDRI
GVCKGIFRTD NVPDEAVVRL VDTFPGQSID FFGALRARVY DDEVRKWVGE IGVENISKRL
VNSREGPPTF DQPKMTIEKL MEYGHMLVQE QENVKRVQLA DKYLSEAALG QANDDAMKTG
AFYGK