RCAF1_MOUSE
ID RCAF1_MOUSE Reviewed; 129 AA.
AC Q9CQT9;
DT 19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Respirasome Complex Assembly Factor 1 {ECO:0000250|UniProtKB:Q9BUV8};
DE Short=RCAF1 {ECO:0000250|UniProtKB:Q9BUV8};
DE AltName: Full=Rab5-interacting protein {ECO:0000312|MGI:MGI:1914638};
DE Short=RIP5 {ECO:0000312|MGI:MGI:1914638};
GN Name=Rab5if {ECO:0000312|MGI:MGI:1914638};
GN Synonyms=Rcaf1 {ECO:0000250|UniProtKB:Q9BUV8};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, INTERACTION WITH NDUFS3; NDUFA4; NDUFV1; NDUFA9; NDUFS8; UQCRC2;
RP COX5A AND COX7C, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=31536960; DOI=10.1016/j.isci.2019.08.057;
RA Moutaoufik M.T., Malty R., Amin S., Zhang Q., Phanse S., Gagarinova A.,
RA Zilocchi M., Hoell L., Minic Z., Gagarinova M., Aoki H., Stockwell J.,
RA Jessulat M., Goebels F., Broderick K., Scott N.E., Vlasblom J., Musso G.,
RA Prasad B., Lamantea E., Garavaglia B., Rajput A., Murayama K., Okazaki Y.,
RA Foster L.J., Bader G.D., Cayabyab F.S., Babu M.;
RT "Rewiring of the Human Mitochondrial Interactome during Neuronal
RT Reprogramming Reveals Regulators of the Respirasome and Neurogenesis.";
RL IScience 19:1114-1132(2019).
CC -!- FUNCTION: Acts as an assembly factor for mitochondrial respiratory
CC complexes. {ECO:0000269|PubMed:31536960}.
CC -!- SUBUNIT: Interacts with NDUFS3, NDUFA4, NDUFV1, NDUFA9 and NDUFS8 of
CC the mitochondrial membrane respiratory chain NADH dehydrogenase
CC (Complex I) (PubMed:31536960). Interacts with UQCRC2 of the ubiquinol-
CC cytochrome c reductase complex (Complex III) (PubMed:31536960).
CC Interacts with COX5A and COX7C of the cytochrome c oxidase complex
CC (Complex IV) (PubMed:31536960). {ECO:0000269|PubMed:31536960}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:31536960}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in neuronal cells.
CC {ECO:0000269|PubMed:31536960}.
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DR EMBL; AK003568; BAB22864.1; -; mRNA.
DR EMBL; AK002452; BAB22111.1; -; mRNA.
DR EMBL; BC019445; AAH19445.1; -; mRNA.
DR CCDS; CCDS16970.1; -.
DR RefSeq; NP_080400.1; NM_026124.3.
DR AlphaFoldDB; Q9CQT9; -.
DR SMR; Q9CQT9; -.
DR STRING; 10090.ENSMUSP00000029165; -.
DR iPTMnet; Q9CQT9; -.
DR PhosphoSitePlus; Q9CQT9; -.
DR EPD; Q9CQT9; -.
DR MaxQB; Q9CQT9; -.
DR PaxDb; Q9CQT9; -.
DR PeptideAtlas; Q9CQT9; -.
DR PRIDE; Q9CQT9; -.
DR Antibodypedia; 77474; 3 antibodies from 1 providers.
DR DNASU; 67388; -.
DR Ensembl; ENSMUST00000029165; ENSMUSP00000029165; ENSMUSG00000027637.
DR GeneID; 67388; -.
DR KEGG; mmu:67388; -.
DR UCSC; uc008nob.2; mouse.
DR CTD; 55969; -.
DR MGI; MGI:1914638; Rab5if.
DR VEuPathDB; HostDB:ENSMUSG00000027637; -.
DR eggNOG; KOG3415; Eukaryota.
DR GeneTree; ENSGT00390000004786; -.
DR HOGENOM; CLU_120704_1_0_1; -.
DR InParanoid; Q9CQT9; -.
DR OMA; ITANSEW; -.
DR OrthoDB; 1562107at2759; -.
DR PhylomeDB; Q9CQT9; -.
DR TreeFam; TF314558; -.
DR BioGRID-ORCS; 67388; 0 hits in 71 CRISPR screens.
DR PRO; PR:Q9CQT9; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9CQT9; protein.
DR Bgee; ENSMUSG00000027637; Expressed in primitive streak and 260 other tissues.
DR Genevisible; Q9CQT9; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005746; C:mitochondrial respirasome; IMP:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0097250; P:mitochondrial respirasome assembly; IMP:UniProtKB.
DR InterPro; IPR029008; EMC6-like.
DR InterPro; IPR010742; RCAF1.
DR PANTHER; PTHR12906; PTHR12906; 1.
DR Pfam; PF07019; EMC6; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..129
FT /note="Respirasome Complex Assembly Factor 1"
FT /id="PRO_0000079416"
FT TRANSMEM 45..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..92
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 129 AA; 14825 MW; 5BF6FB71C569F0CD CRC64;
MSGGRRKEEP PQPQLANGAL KVSVWSKVLR SDAAWDDKDE FLDVIYWFRQ IIALVLGVIW
GVLPLRGFLG IAGFCLINAG VLYLYFSNYL QIDEEEYGGT WELTKEGFMT SFALFMVIWI
IFYTAIHYD
