RCAN1_MOUSE
ID RCAN1_MOUSE Reviewed; 251 AA.
AC Q9JHG6; Q7TNY3; Q91WQ4; Q9JK50; Q9JK51; Q9JKK2; Q9JKK3;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 23-MAY-2018, sequence version 2.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Calcipressin-1;
DE AltName: Full=Down syndrome critical region protein 1 homolog;
DE AltName: Full=Myocyte-enriched calcineurin-interacting protein 1;
DE Short=MCIP1;
DE AltName: Full=Regulator of calcineurin 1;
GN Name=Rcan1; Synonyms=Dscr1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS B AND E).
RX PubMed=10722714; DOI=10.1074/jbc.275.12.8719;
RA Rothermel B., Vega R.B., Yang J., Wu H., Bassel-Duby R., Williams R.S.;
RT "A protein encoded within the Down syndrome critical region is enriched in
RT striated muscles and inhibits calcineurin signaling.";
RL J. Biol. Chem. 275:8719-8725(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM E), FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Fetal brain;
RX PubMed=11231093; DOI=10.1016/s0925-4773(00)00583-9;
RA Casas C., Martinez S., Pritchard M.A., Fuentes J.J., Nadal M., Guimera J.,
RA Arbones M., Florez J., Soriano E., Estivill X., Alcantara S.;
RT "Dscr1, a novel endogenous inhibitor of calcineurin signaling, is expressed
RT in the primitive ventricle of the heart and during neurogenesis.";
RL Mech. Dev. 101:289-292(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), INTERACTION WITH PPP3CA,
RP PHOSPHORYLATION, AND TISSUE SPECIFICITY.
RC STRAIN=NIH Swiss; TISSUE=Heart;
RX PubMed=12809556; DOI=10.1042/bj20030267;
RA Genesca L., Aubareda A., Fuentes J.J., Estivill X., De La Luna S.,
RA Perez-Riba M.;
RT "Phosphorylation of calcipressin 1 increases its ability to inhibit
RT calcineurin and decreases calcipressin half-life.";
RL Biochem. J. 374:567-575(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS B AND C).
RA Fuentes J.J., Pritchard M.A., Pucharcos C., Estivill X.;
RT "Down syndrome candidate region 1 (Dscr1), one of three alternatively
RT spliced exon 1 transcripts.";
RL Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND C).
RC STRAIN=C57BL/6J; TISSUE=Embryonic stem cell;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J; TISSUE=Heart;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 52-251 (ISOFORM A).
RC STRAIN=BALB/cJ; TISSUE=Brain;
RX PubMed=11080588; DOI=10.1016/s0378-1119(00)00407-8;
RA Strippoli P., Petrini M., Lenzi L., Carinci P., Zannotti M.;
RT "The murine DSCR1-like (Down syndrome candidate region 1) gene family:
RT conserved synteny with the human orthologous genes.";
RL Gene 257:223-232(2000).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [10]
RP STRUCTURE BY NMR OF 67-156.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of RRM domain in calcipressin 1.";
RL Submitted (MAY-2004) to the PDB data bank.
CC -!- FUNCTION: Inhibits calcineurin-dependent transcriptional responses by
CC binding to the catalytic domain of calcineurin A. Could play a role
CC during central nervous system development (PubMed:11231093).
CC {ECO:0000269|PubMed:11231093}.
CC -!- SUBUNIT: Interacts with RAF1 and PPP3R1 (By similarity). Interacts with
CC PPP3CA (PubMed:12809556). {ECO:0000250|UniProtKB:P53805,
CC ECO:0000269|PubMed:12809556}.
CC -!- INTERACTION:
CC Q9JHG6; Q61214: Dyrk1a; NbExp=2; IntAct=EBI-644061, EBI-80344;
CC Q9JHG6; P35922: Fmr1; NbExp=3; IntAct=EBI-644061, EBI-645094;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Comment=Additional isoforms seem to exist.;
CC Name=A; Synonyms=1, CALP1-L {ECO:0000303|PubMed:12809556};
CC IsoId=Q9JHG6-1; Sequence=Displayed;
CC Name=B; Synonyms=4;
CC IsoId=Q9JHG6-2; Sequence=VSP_001317;
CC Name=C;
CC IsoId=Q9JHG6-3; Sequence=VSP_001318;
CC Name=E; Synonyms=CALP1-S {ECO:0000303|PubMed:12809556};
CC IsoId=Q9JHG6-4; Sequence=VSP_059568;
CC -!- TISSUE SPECIFICITY: Highly expressed in heart and skeletal muscle. Also
CC expressed in all other tissues. {ECO:0000269|PubMed:11231093,
CC ECO:0000269|PubMed:12809556}.
CC -!- PTM: Phosphorylation increases its ability to inhibit calcineurin and
CC decreases protein half-life. {ECO:0000250|UniProtKB:P53805}.
CC -!- SIMILARITY: Belongs to the RCAN family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF91461.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF237789; AAF63485.1; -; mRNA.
DR EMBL; AF237790; AAF63486.1; -; mRNA.
DR EMBL; AF260717; AAF70343.1; -; mRNA.
DR EMBL; AY325904; AAP96744.1; -; mRNA.
DR EMBL; AF263239; AAF72701.1; -; mRNA.
DR EMBL; AF263240; AAF72702.1; -; mRNA.
DR EMBL; AK010696; BAB27128.1; -; mRNA.
DR EMBL; AK146764; BAE27416.1; -; mRNA.
DR EMBL; AC162305; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC174448; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC013551; AAH13551.1; -; mRNA.
DR EMBL; AF282255; AAF91461.1; ALT_INIT; mRNA.
DR CCDS; CCDS28337.1; -. [Q9JHG6-2]
DR CCDS; CCDS37405.1; -. [Q9JHG6-1]
DR RefSeq; NP_001075018.1; NM_001081549.2. [Q9JHG6-1]
DR RefSeq; NP_062339.2; NM_019466.4. [Q9JHG6-2]
DR PDB; 1WEY; NMR; -; A=67-156.
DR PDBsum; 1WEY; -.
DR AlphaFoldDB; Q9JHG6; -.
DR SMR; Q9JHG6; -.
DR BioGRID; 207725; 2.
DR IntAct; Q9JHG6; 4.
DR MINT; Q9JHG6; -.
DR STRING; 10090.ENSMUSP00000060394; -.
DR iPTMnet; Q9JHG6; -.
DR PhosphoSitePlus; Q9JHG6; -.
DR jPOST; Q9JHG6; -.
DR MaxQB; Q9JHG6; -.
DR PaxDb; Q9JHG6; -.
DR PRIDE; Q9JHG6; -.
DR ProteomicsDB; 255166; -. [Q9JHG6-1]
DR ProteomicsDB; 255167; -. [Q9JHG6-2]
DR ProteomicsDB; 255168; -. [Q9JHG6-3]
DR ProteomicsDB; 329214; -.
DR Antibodypedia; 22959; 580 antibodies from 35 providers.
DR DNASU; 54720; -.
DR Ensembl; ENSMUST00000023672; ENSMUSP00000023672; ENSMUSG00000022951. [Q9JHG6-2]
DR Ensembl; ENSMUST00000060005; ENSMUSP00000060394; ENSMUSG00000022951. [Q9JHG6-1]
DR Ensembl; ENSMUST00000231410; ENSMUSP00000156110; ENSMUSG00000022951. [Q9JHG6-3]
DR Ensembl; ENSMUST00000232197; ENSMUSP00000155863; ENSMUSG00000022951. [Q9JHG6-3]
DR Ensembl; ENSMUST00000232239; ENSMUSP00000156053; ENSMUSG00000022951. [Q9JHG6-4]
DR GeneID; 54720; -.
DR KEGG; mmu:54720; -.
DR UCSC; uc007zze.2; mouse.
DR CTD; 1827; -.
DR MGI; MGI:1890564; Rcan1.
DR VEuPathDB; HostDB:ENSMUSG00000022951; -.
DR eggNOG; KOG4019; Eukaryota.
DR GeneTree; ENSGT00940000159870; -.
DR HOGENOM; CLU_076190_2_0_1; -.
DR InParanoid; Q9JHG6; -.
DR OMA; DMPPVVC; -.
DR OrthoDB; 1585262at2759; -.
DR PhylomeDB; Q9JHG6; -.
DR TreeFam; TF313579; -.
DR BioGRID-ORCS; 54720; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Rcan1; mouse.
DR EvolutionaryTrace; Q9JHG6; -.
DR PRO; PR:Q9JHG6; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q9JHG6; protein.
DR Bgee; ENSMUSG00000022951; Expressed in epithelium of lens and 287 other tissues.
DR ExpressionAtlas; Q9JHG6; baseline and differential.
DR Genevisible; Q9JHG6; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0008597; F:calcium-dependent protein serine/threonine phosphatase regulator activity; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0033173; P:calcineurin-NFAT signaling cascade; IMP:MGI.
DR GO; GO:0019722; P:calcium-mediated signaling; IBA:GO_Central.
DR GO; GO:0031987; P:locomotion involved in locomotory behavior; IGI:MGI.
DR GO; GO:0070885; P:negative regulation of calcineurin-NFAT signaling cascade; ISS:UniProtKB.
DR GO; GO:0051151; P:negative regulation of smooth muscle cell differentiation; ISO:MGI.
DR GO; GO:0043666; P:regulation of phosphoprotein phosphatase activity; IDA:MGI.
DR GO; GO:0043627; P:response to estrogen; ISO:MGI.
DR GO; GO:0002931; P:response to ischemia; IMP:MGI.
DR GO; GO:0006979; P:response to oxidative stress; IGI:MGI.
DR GO; GO:0007614; P:short-term memory; IGI:MGI.
DR GO; GO:0048741; P:skeletal muscle fiber development; IDA:MGI.
DR CDD; cd12708; RRM_RCAN1; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR006931; Calcipressin.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR031271; RCAN1.
DR InterPro; IPR034906; RCAN1_RRM.
DR PANTHER; PTHR10300; PTHR10300; 1.
DR PANTHER; PTHR10300:SF4; PTHR10300:SF4; 1.
DR Pfam; PF04847; Calcipressin; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..251
FT /note="Calcipressin-1"
FT /id="PRO_0000211415"
FT REGION 216..251
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 225..239
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 161
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53805"
FT MOD_RES 165
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53805"
FT MOD_RES 216
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..133
FT /note="Missing (in isoform C)"
FT /evidence="ECO:0000303|PubMed:16141072, ECO:0000303|Ref.4"
FT /id="VSP_001318"
FT VAR_SEQ 1..81
FT /note="MEDGVAGPRLGEVAEAVEARAPRRVTLRPFAPFSAAAEGDGGGGGDWSFIDC
FT EMEEVDLQDLPSATIACHLDPRVFVDGLC -> MHFRDFSYNFSSLIACVANDDVFSES
FT ET (in isoform B)"
FT /evidence="ECO:0000303|PubMed:10722714,
FT ECO:0000303|PubMed:15489334, ECO:0000303|Ref.4"
FT /id="VSP_001317"
FT VAR_SEQ 1..53
FT /note="Missing (in isoform E)"
FT /evidence="ECO:0000303|PubMed:10722714,
FT ECO:0000303|PubMed:11231093"
FT /id="VSP_059568"
FT CONFLICT 205
FT /note="T -> P (in Ref. 1; AAF63485/AAF63486)"
FT /evidence="ECO:0000305"
FT STRAND 67..69
FT /evidence="ECO:0007829|PDB:1WEY"
FT HELIX 73..76
FT /evidence="ECO:0007829|PDB:1WEY"
FT TURN 78..80
FT /evidence="ECO:0007829|PDB:1WEY"
FT HELIX 81..90
FT /evidence="ECO:0007829|PDB:1WEY"
FT STRAND 97..101
FT /evidence="ECO:0007829|PDB:1WEY"
FT TURN 102..105
FT /evidence="ECO:0007829|PDB:1WEY"
FT STRAND 106..110
FT /evidence="ECO:0007829|PDB:1WEY"
FT HELIX 116..122
FT /evidence="ECO:0007829|PDB:1WEY"
FT STRAND 127..129
FT /evidence="ECO:0007829|PDB:1WEY"
FT STRAND 135..137
FT /evidence="ECO:0007829|PDB:1WEY"
FT CONFLICT Q9JHG6-2:2
FT /note="H -> D (in Ref. 1; AAF63486)"
FT /evidence="ECO:0000305"
FT CONFLICT Q9JHG6-2:6
FT /note="F -> Y (in Ref. 4; AAF72701)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 251 AA; 28137 MW; 09F47C73D847FB2B CRC64;
MEDGVAGPRL GEVAEAVEAR APRRVTLRPF APFSAAAEGD GGGGGDWSFI DCEMEEVDLQ
DLPSATIACH LDPRVFVDGL CRAKFESLFR TYDKDTTFQY FKSFKRVRIN FSNPLSAADA
RLRLHKTEFL GKEMKLYFAQ TLHIGSSHLA PPNPDKQFLI SPPASPPVGW KQVEDATPVI
NYDLLYAISK LGPGEKYELH AATDTTPSVV VHVCESDQEN EEEEEEMERM KRPKPKIIQT
RRPEYTPIHL S
