RCA_ORYSJ
ID RCA_ORYSJ Reviewed; 466 AA.
AC P93431; A3CE17; B7EAB2; Q0IQU7; Q53MC4; Q53MC5; Q53NV9; Q7XXR6; Q9LRH9;
AC Q9LRI0;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2006, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic;
DE Short=RA;
DE Short=RuBisCO activase;
DE Flags: Precursor;
GN Name=RCA; Synonyms=RCAA1, RCAA2;
GN OrderedLocusNames=Os11g0707000, LOC_Os11g47970; ORFNames=OsJ_033539;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC STRAIN=cv. Tainung 67; TISSUE=Leaf;
RA To K.-Y., Suen D.-F., Cheng M.-C., Chen L.-F.O., Chen S.C.-G.;
RT "Cloning and sequencing of a rice cDNA encoding Rubisco activase.";
RL (er) Plant Gene Register PGR97-011(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Panicle;
RX PubMed=15659629; DOI=10.1105/tpc.104.028456;
RA Moriguchi K., Suzuki T., Ito Y., Yamazaki Y., Niwa Y., Kurata N.;
RT "Functional isolation of novel nuclear proteins showing a variety of
RT subnuclear localizations.";
RL Plant Cell 17:389-403(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Nipponbare;
RA Komatsu S., Zhang Z.;
RT "Oryza sativa OsrcaA2 mRNA for RuBisCO activase small isoform precursor.";
RL Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [7]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [10]
RP PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 49-56 AND 87-93, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=cv. Nipponbare;
RX PubMed=16758443; DOI=10.1002/pmic.200600043;
RA Nozu Y., Tsugita A., Kamijo K.;
RT "Proteomic analysis of rice leaf, stem and root tissues during growth
RT course.";
RL Proteomics 6:3665-3670(2006).
CC -!- FUNCTION: Activation of RuBisCO (ribulose-1,5-bisphosphate
CC carboxylase/oxygenase; EC 4.1.1.39) involves the ATP-dependent
CC carboxylation of the epsilon-amino group of lysine leading to a
CC carbamate structure.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=RCAA1;
CC IsoId=P93431-1; Sequence=Displayed;
CC Name=2; Synonyms=RCAA2;
CC IsoId=P93431-2; Sequence=VSP_019480, VSP_019481;
CC -!- SIMILARITY: Belongs to the RuBisCO activase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAX95285.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAX95414.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF28918.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U74321; AAC28134.1; -; mRNA.
DR EMBL; AB110180; BAC78572.1; -; mRNA.
DR EMBL; AB034698; BAA97583.1; -; mRNA.
DR EMBL; AB034748; BAA97584.1; -; mRNA.
DR EMBL; AC133008; AAX95414.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC137064; AAX95285.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC137064; AAX95286.1; -; Genomic_DNA.
DR EMBL; DP000010; ABA95522.1; -; Genomic_DNA.
DR EMBL; DP000010; ABA95523.1; -; Genomic_DNA.
DR EMBL; AP008217; BAF28918.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014967; BAT15415.1; -; Genomic_DNA.
DR EMBL; CM000148; EAZ19330.1; -; Genomic_DNA.
DR EMBL; AK064986; BAG89309.1; -; mRNA.
DR PIR; T04160; T04160.
DR RefSeq; XP_015616897.1; XM_015761411.1. [P93431-1]
DR AlphaFoldDB; P93431; -.
DR SMR; P93431; -.
DR BioGRID; 820261; 1.
DR IntAct; P93431; 1.
DR STRING; 4530.OS11T0707000-02; -.
DR PaxDb; P93431; -.
DR PRIDE; P93431; -.
DR EnsemblPlants; Os11t0707000-02; Os11t0707000-02; Os11g0707000. [P93431-2]
DR GeneID; 4351224; -.
DR Gramene; Os11t0707000-02; Os11t0707000-02; Os11g0707000. [P93431-2]
DR KEGG; osa:4351224; -.
DR eggNOG; KOG0651; Eukaryota.
DR InParanoid; P93431; -.
DR OMA; MEKFYWE; -.
DR OrthoDB; 655049at2759; -.
DR Proteomes; UP000000763; Chromosome 11.
DR Proteomes; UP000007752; Chromosome 11.
DR Proteomes; UP000059680; Chromosome 11.
DR Genevisible; P93431; OS.
DR GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0046863; F:ribulose-1,5-bisphosphate carboxylase/oxygenase activator activity; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR044960; RCA-like.
DR PANTHER; PTHR32429; PTHR32429; 1.
DR Pfam; PF00004; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Chloroplast; Direct protein sequencing;
KW Nucleotide-binding; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..48
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:16758443"
FT CHAIN 49..466
FT /note="Ribulose bisphosphate carboxylase/oxygenase
FT activase, chloroplastic"
FT /id="PRO_0000030238"
FT REGION 429..454
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 156..163
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VAR_SEQ 429..433
FT /note="QGAQQ -> SAPSS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764, ECO:0000303|Ref.1,
FT ECO:0000303|Ref.3"
FT /id="VSP_019480"
FT VAR_SEQ 434..466
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764, ECO:0000303|Ref.1,
FT ECO:0000303|Ref.3"
FT /id="VSP_019481"
FT CONFLICT 127
FT /note="A -> S (in Ref. 1; AAC28134)"
FT /evidence="ECO:0000305"
FT CONFLICT 190
FT /note="Missing (in Ref. 1; AAC28134)"
FT /evidence="ECO:0000305"
FT CONFLICT 385
FT /note="M -> I (in Ref. 1; AAC28134)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 466 AA; 51454 MW; 81AF76377BE148E1 CRC64;
MAAAFSSTVG APASTPTNFL GKKLKKQVTS AVNYHGKSSN INRFKVMAKE LDEGKQTDQD
RWKGLAYDIS DDQQDITRGK GFVDSLFQAP TGDGTHEAVL SSYEYLSQGL RTYDFDNTMG
GFYIAPAFMD KLVVHISKNF MTLPNIKVPL ILGIWGGKGQ GKSFQCELVF AKMGINPIMM
SAGELESGNA GEPAKLIRQR YREAADIIKK GKMCCLFIND LDAGAGRMGG TTQYTVNNQM
VNATLMNIAD NPTNVQLPGM YNKEDNPRVP IIVTGNDFST LYAPLIRDGR MEKFYWAPTR
DDRVGVCKGI FRTDNVPDED IVKIVDSFPG QSIDFFGALR ARVYDDEVRK WVSDTGVENI
GKRLVNSREG PPEFEQPKMT IEKLMEYGYM LVKEQENVKR VQLAEQYLSE AALGDANSDA
MKTGSFYGQG AQQAGNLPVP EGCTDPVAKN FDPTARSDDG SCLYTF
