ID   RCA_SPIOL               Reviewed;         472 AA.
AC   P10871; Q43327;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic;
DE            Short=RA;
DE            Short=RuBisCO activase;
DE   Flags: Precursor;
OS   Spinacia oleracea (Spinach).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX   NCBI_TaxID=3562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   TISSUE=Leaf;
RX   PubMed=3277181; DOI=10.1073/pnas.85.3.787;
RA   Werneke J.M., Zielinski R.E., Ogren W.L.;
RT   "Structure and expression of spinach leaf cDNA encoding
RT   ribulosebisphosphate carboxylase/oxygenase activase.";
RL   Proc. Natl. Acad. Sci. U.S.A. 85:787-791(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE, AND ALTERNATIVE SPLICING.
RX   PubMed=2535524; DOI=10.2307/3868990;
RA   Werneke J.M., Chatfield J.M., Ogren W.L.;
RT   "Alternative mRNA splicing generates the two ribulosebisphosphate
RT   carboxylase/oxygenase activase polypeptides in spinach and Arabidopsis.";
RL   Plant Cell 1:815-825(1989).
CC   -!- FUNCTION: Activation of RuBisCO (ribulose-1,5-bisphosphate
CC       carboxylase/oxygenase; EC 4.1.1.39) involves the ATP-dependent
CC       carboxylation of the epsilon-amino group of lysine leading to a
CC       carbamate structure.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1; Synonyms=45 kDa;
CC         IsoId=P10871-1; Sequence=Displayed;
CC       Name=2; Synonyms=41 kDa;
CC         IsoId=P10871-2; Sequence=VSP_005541;
CC   -!- SIMILARITY: Belongs to the RuBisCO activase family. {ECO:0000305}.
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DR   EMBL; J03610; AAA34038.1; -; mRNA.
DR   EMBL; S45033; AAD13840.1; -; Genomic_DNA.
DR   EMBL; S45033; AAD13841.1; -; Genomic_DNA.
DR   PIR; A31082; A31082.
DR   AlphaFoldDB; P10871; -.
DR   SMR; P10871; -.
DR   IntAct; P10871; 1.
DR   PRIDE; P10871; -.
DR   GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR044960; RCA-like.
DR   PANTHER; PTHR32429; PTHR32429; 1.
DR   Pfam; PF00004; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; ATP-binding; Chloroplast; Direct protein sequencing;
KW   Nucleotide-binding; Plastid; Transit peptide.
FT   TRANSIT         1..58
FT                   /note="Chloroplast"
FT   CHAIN           59..472
FT                   /note="Ribulose bisphosphate carboxylase/oxygenase
FT                   activase, chloroplastic"
FT                   /id="PRO_0000030241"
FT   REGION          448..472
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         163..170
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         436..472
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_005541"
FT   CONFLICT        129
FT                   /note="F -> L (in Ref. 1; AAA34038)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        253
FT                   /note="M -> L (in Ref. 1; AAA34038)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        352
FT                   /note="H -> D (in Ref. 1; AAA34038)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   472 AA;  51559 MW;  D6D04A3EF6E3489E CRC64;
     MATAVSTVGA ATRAPLNLNG SSAGASVPTS GFLGSSLKKH TNVRFPSSSR TTSMTVKAAE
     NEEKNTDKWA HLAKDFSDDQ LDIRRGKGMV DSLFQAPADA GTHVPIQSSF EYESQGLRKY
     DIDNMLGDFY IAPAFMDKLV VHITKNFLNL PNIKIPLILG VWGGKGQGKS FQCELVFAKL
     GINPIMMSAG ELESGNAGEP AKLIRQRYRE AADLIAKGKM CALFINDLEP GAGRMGGTTQ
     YTVNNQMVNA TLMNIADNPT NVQLPGMYNK QDNARVPIIV TGNDFSTLYA PLIRDGRMEK
     FYWAPTREDR IGVCTGIFKT DKVPAEHVVK LVDAFPGQSI DFFGALRARV YHDEVRKWVN
     SVGVDNVGKK LVNSKDGPPV FEQPEMTLQK LMEYGNMLVQ EQENVKRVQL ADQYMSSAAL
     GDANKDAIDR GTFFGKAAQQ VSLPVAQGCT DPEAKNYDPT ARSDDGSCTY NL