RCBA_ECOLI
ID RCBA_ECOLI Reviewed; 69 AA.
AC P33230; P77683;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Double-strand break reduction protein {ECO:0000303|PubMed:22343303};
GN Name=rcbA {ECO:0000303|PubMed:22343303}; Synonyms=ydaC;
GN OrderedLocusNames=b1347, JW1341;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-62.
RC STRAIN=K12;
RX PubMed=8244937; DOI=10.1128/jb.175.23.7673-7682.1993;
RA Clark A.J., Sharma V., Brenowitz S., Chu C.C., Sandler S.J., Satin L.,
RA Templin A., Berger I., Cohen A.;
RT "Genetic and molecular analyses of the C-terminal region of the recE gene
RT from the Rac prophage of Escherichia coli K-12 reveal the recT gene.";
RL J. Bacteriol. 175:7673-7682(1993).
RN [5]
RP FUNCTION IN REDUCTION OF CHROMOSOME BREAKS, DISRUPTION PHENOTYPE, AND GENE
RP NAME.
RX PubMed=22343303; DOI=10.1128/jb.06390-11;
RA Felczak M.M., Kaguni J.M.;
RT "The rcbA gene product reduces spontaneous and induced chromosome breaks in
RT Escherichia coli.";
RL J. Bacteriol. 194:2152-2164(2012).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, ANTIBIOTIC RESISTANCE, AND MUTAGENESIS OF
RP MET-1 AND 9-GLU--ASN-69.
RC STRAIN=K12 / BW25113;
RX PubMed=24748661; DOI=10.1093/nar/gku279;
RA Guo Y., Quiroga C., Chen Q., McAnulty M.J., Benedik M.J., Wood T.K.,
RA Wang X.;
RT "RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system
RT in Escherichia coli.";
RL Nucleic Acids Res. 42:6448-6462(2014).
CC -!- FUNCTION: Helps to maintain the integrity of the chromosome by lowering
CC the steady-state level of double strand breaks (PubMed:22343303). This
CC region of DNA acts as an antitoxin to toxin RalR, a DNase, but it seems
CC to be sRNA RalA that has the antitoxin activity and not this putative
CC protein. Therefore the identity of this as a protein-coding gene has
CC been cast into doubt (PubMed:24748661). {ECO:0000269|PubMed:22343303,
CC ECO:0000269|PubMed:24748661}.
CC -!- INTERACTION:
CC P33230; P75728: ubiF; NbExp=2; IntAct=EBI-562744, EBI-562588;
CC -!- DISRUPTION PHENOTYPE: Mutants show increased numbers of double-strand
CC breaks (PubMed:22343303). The single ralR and double ralR-ralA mutant
CC have increased sensitivity to the antibiotic fosfomycin.
CC {ECO:0000269|PubMed:22343303, ECO:0000269|PubMed:24748661}.
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DR EMBL; U00096; AAC74429.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA14947.1; -; Genomic_DNA.
DR EMBL; L23927; AAA16181.1; -; Unassigned_DNA.
DR PIR; F64884; F64884.
DR RefSeq; NP_415863.1; NC_000913.3.
DR RefSeq; WP_000276809.1; NZ_CP047127.1.
DR AlphaFoldDB; P33230; -.
DR BioGRID; 4261948; 13.
DR DIP; DIP-11619N; -.
DR IntAct; P33230; 5.
DR STRING; 511145.b1347; -.
DR PaxDb; P33230; -.
DR PRIDE; P33230; -.
DR EnsemblBacteria; AAC74429; AAC74429; b1347.
DR EnsemblBacteria; BAA14947; BAA14947; BAA14947.
DR GeneID; 60668624; -.
DR GeneID; 66674825; -.
DR GeneID; 947504; -.
DR KEGG; ecj:JW1341; -.
DR KEGG; eco:b1347; -.
DR PATRIC; fig|1411691.4.peg.929; -.
DR EchoBASE; EB1846; -.
DR eggNOG; ENOG503488V; Bacteria.
DR HOGENOM; CLU_185969_0_0_6; -.
DR OMA; CKENPPA; -.
DR BioCyc; EcoCyc:EG11901-MON; -.
DR PRO; PR:P33230; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0006259; P:DNA metabolic process; IMP:EcoCyc.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR InterPro; IPR009572; DUF1187.
DR Pfam; PF06688; DUF1187; 1.
PE 1: Evidence at protein level;
KW Antibiotic resistance; Reference proteome.
FT CHAIN 1..69
FT /note="Double-strand break reduction protein"
FT /id="PRO_0000168901"
FT MUTAGEN 1
FT /note="M->T: No protein should be translated, DNA region
FT still acts as an antitoxin to RalR."
FT /evidence="ECO:0000269|PubMed:24748661"
FT MUTAGEN 9..69
FT /note="Missing: No protein should be translated, DNA region
FT still acts as an antitoxin to RalR."
FT /evidence="ECO:0000269|PubMed:24748661"
SQ SEQUENCE 69 AA; 7863 MW; D65D6454A7FBF4A6 CRC64;
MYKITATIEK EGGTPTNWTR YSKSKLTKSE CEKMLSGKKE AGVSREQKVK LINFNCEKLQ
SSRIALYSN
