RCC1_DROME
ID RCC1_DROME Reviewed; 547 AA.
AC P25171; A4V1J1; Q9VRR5;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 2.
DT 03-AUG-2022, entry version 184.
DE RecName: Full=Regulator of chromosome condensation;
DE AltName: Full=Chromatin-binding protein Bj1;
DE AltName: Full=Regulator of chromosome condensation 1 ortholog;
GN Name=Rcc1; Synonyms=Bj1; ORFNames=CG10480;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2022188; DOI=10.1002/j.1460-2075.1991.tb08064.x;
RA Frasch M.;
RT "The maternally expressed Drosophila gene encoding the chromatin-binding
RT protein BJ1 is a homolog of the vertebrate gene Regulator of Chromatin
RT Condensation, RCC1.";
RL EMBO J. 10:1225-1236(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=2022190; DOI=10.1002/j.1460-2075.1991.tb08068.x;
RA Ohtsubo M., Yoshida T., Seino H., Nishitani H., Clark K.L.,
RA Sprague G.F. Jr., Frasch M., Nishimoto T.;
RT "Mutation of the hamster cell cycle gene RCC1 is complemented by the
RT homologous genes of Drosophila and S.cerevisiae.";
RL EMBO J. 10:1265-1273(1991).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=12121620; DOI=10.1016/s0960-9822(02)00934-x;
RA Trieselmann N., Wilde A.;
RT "Ran localizes around the microtubule spindle in vivo during mitosis in
RT Drosophila embryos.";
RL Curr. Biol. 12:1124-1129(2002).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-286; THR-512; SER-526 AND
RP THR-531, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Promotes the exchange of Ran-bound GDP by GTP. Involved in
CC the regulation of onset of chromosome condensation in the S phase.
CC Binds to chromatin. {ECO:0000269|PubMed:2022190}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Becomes dispersed
CC throughout the cytoplasm during mitosis. Is associated with chromatin
CC throughout mitosis.
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DR EMBL; X58530; CAA41417.1; -; Genomic_DNA.
DR EMBL; AE014296; AAF50726.1; -; Genomic_DNA.
DR EMBL; AE014296; AAF50727.1; -; Genomic_DNA.
DR EMBL; AE014296; AAN12235.1; -; Genomic_DNA.
DR EMBL; AY070540; AAL48011.1; -; mRNA.
DR PIR; S15028; S15028.
DR RefSeq; NP_523943.1; NM_079219.3.
DR RefSeq; NP_729118.1; NM_168151.3.
DR RefSeq; NP_729119.1; NM_168152.3.
DR PDB; 3MVD; X-ray; 2.90 A; K/L=2-422.
DR PDBsum; 3MVD; -.
DR AlphaFoldDB; P25171; -.
DR SMR; P25171; -.
DR BioGRID; 64129; 13.
DR DIP; DIP-17766N; -.
DR IntAct; P25171; 6.
DR STRING; 7227.FBpp0076782; -.
DR iPTMnet; P25171; -.
DR PaxDb; P25171; -.
DR PRIDE; P25171; -.
DR DNASU; 38669; -.
DR EnsemblMetazoa; FBtr0077074; FBpp0076782; FBgn0002638.
DR EnsemblMetazoa; FBtr0077075; FBpp0076783; FBgn0002638.
DR EnsemblMetazoa; FBtr0077076; FBpp0076784; FBgn0002638.
DR GeneID; 38669; -.
DR KEGG; dme:Dmel_CG10480; -.
DR CTD; 1104; -.
DR FlyBase; FBgn0002638; Rcc1.
DR VEuPathDB; VectorBase:FBgn0002638; -.
DR eggNOG; KOG1426; Eukaryota.
DR GeneTree; ENSGT00940000174254; -.
DR HOGENOM; CLU_005210_6_1_1; -.
DR InParanoid; P25171; -.
DR OMA; WSWGTND; -.
DR OrthoDB; 1062377at2759; -.
DR PhylomeDB; P25171; -.
DR Reactome; R-DME-9615933; Postmitotic nuclear pore complex (NPC) reformation.
DR SignaLink; P25171; -.
DR BioGRID-ORCS; 38669; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Rcc1; fly.
DR GenomeRNAi; 38669; -.
DR PRO; PR:P25171; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0002638; Expressed in cleaving embryo and 47 other tissues.
DR Genevisible; P25171; DM.
DR GO; GO:0000793; C:condensed chromosome; IDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; IDA:UniProtKB.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:FlyBase.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007417; P:central nervous system development; IDA:UniProtKB.
DR GO; GO:0006607; P:NLS-bearing protein import into nucleus; IMP:FlyBase.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; IDA:UniProtKB.
DR GO; GO:0050767; P:regulation of neurogenesis; IMP:FlyBase.
DR GO; GO:0046822; P:regulation of nucleocytoplasmic transport; IMP:FlyBase.
DR GO; GO:0007419; P:ventral cord development; HMP:FlyBase.
DR Gene3D; 2.130.10.30; -; 1.
DR InterPro; IPR009091; RCC1/BLIP-II.
DR InterPro; IPR000408; Reg_chr_condens.
DR Pfam; PF00415; RCC1; 4.
DR PRINTS; PR00633; RCCNDNSATION.
DR SUPFAM; SSF50985; SSF50985; 1.
DR PROSITE; PS00625; RCC1_1; 1.
DR PROSITE; PS00626; RCC1_2; 2.
DR PROSITE; PS50012; RCC1_3; 6.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell division; Cytoplasm;
KW Guanine-nucleotide releasing factor; Mitosis; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat.
FT CHAIN 1..547
FT /note="Regulator of chromosome condensation"
FT /id="PRO_0000206633"
FT REPEAT 41..91
FT /note="RCC1 1"
FT REPEAT 92..143
FT /note="RCC1 2"
FT REPEAT 145..195
FT /note="RCC1 3"
FT REPEAT 197..257
FT /note="RCC1 4"
FT REPEAT 260..310
FT /note="RCC1 5"
FT REPEAT 311..362
FT /note="RCC1 6"
FT REPEAT 363..416
FT /note="RCC1 7"
FT REPEAT 417..454
FT /note="Bj1 1"
FT REPEAT 455..489
FT /note="Bj1 2"
FT REPEAT 490..519
FT /note="Bj1 3"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 423..547
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 426..441
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 442..463
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 478..498
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 286
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 512
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 526
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 531
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 490
FT /note="V -> A (in Ref. 1; CAA41417)"
FT /evidence="ECO:0000305"
FT STRAND 42..49
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 63..69
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 74..81
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 83..90
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 95..99
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 102..104
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 115..118
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 128..133
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 135..142
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 147..151
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 156..163
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 168..175
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 180..185
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 187..194
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 199..203
FT /evidence="ECO:0007829|PDB:3MVD"
FT TURN 215..219
FT /evidence="ECO:0007829|PDB:3MVD"
FT TURN 223..229
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 233..235
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 243..249
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 252..257
FT /evidence="ECO:0007829|PDB:3MVD"
FT TURN 258..260
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 263..268
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 282..290
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 294..300
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 302..309
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 314..318
FT /evidence="ECO:0007829|PDB:3MVD"
FT HELIX 321..323
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 327..329
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 334..339
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 347..353
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 356..361
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 366..370
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 377..382
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 385..390
FT /evidence="ECO:0007829|PDB:3MVD"
FT TURN 394..398
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 401..406
FT /evidence="ECO:0007829|PDB:3MVD"
FT STRAND 408..416
FT /evidence="ECO:0007829|PDB:3MVD"
SQ SEQUENCE 547 AA; 58851 MW; 77D9744EBAF4597A CRC64;
MPRRKALTNN NNAGEAEQQP PKAKRARIAF HLELPKRRTV LGNVLVCGNG DVGQLGLGED
ILERKRLSPV AGIPDAVDIS AGGMHNLVLT KSGDIYSFGC NDEGALGRDT SEDGSESKPD
LIDLPGKALC ISAGDSHSAC LLEDGRVFAW GSFRDSHGNM GLTIDGNKRT PIDLMEGTVC
CSIASGADHL VILTTAGKVF TVGCAEQGQL GRLSERSISG EGRRGKRDLL RPTQLIITRA
KPFEAIWATN YCTFMRESQT QVIWATGLNN FKQLAHETKG KEFALTPIKT ELKDIRHIAG
GQHHTVILTT DLKCSVVGRP EYGRLGLGDV KDVVEKPTIV KKLTEKIVSV GCGEVCSYAV
TIDGKLYSWG SGVNNQLGVG DGDDELEPIV VVSKNTQGKH MLLASGGGQH AIFLVKADKQ
DQKENVPVKV SGSSSISKKD KTPPQDNVDK EAENVDKQEQ KENLPAKAST SSKKNKTPPQ
DNADKEAENV DKQEQKENLP AKASTSSKKI KTPPQDDAAE EVEEESAQEP TPKKAKKPAA
KRGGKKT
