RCCR1_ORYSJ
ID RCCR1_ORYSJ Reviewed; 329 AA.
AC Q338P6; Q0IXZ5; Q94HB3; Q9AYE6;
DT 22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Red chlorophyll catabolite reductase 1, chloroplastic {ECO:0000303|PubMed:21807436};
DE Short=OsRCCR1 {ECO:0000303|PubMed:21807436};
DE Short=RCC reductase {ECO:0000305};
DE EC=1.3.7.12 {ECO:0000250|UniProtKB:Q8LDU4};
DE Flags: Precursor;
GN Name=RCCR1 {ECO:0000303|PubMed:21807436};
GN OrderedLocusNames=Os10g0389200 {ECO:0000312|EMBL:BAT10653.1},
GN LOC_Os10g25030 {ECO:0000312|EMBL:ABB47486.1};
GN ORFNames=OSJNBa0094J09.24 {ECO:0000312|EMBL:AAK13108.1},
GN OSJNBb0061I18.22 {ECO:0000312|EMBL:AAK52126.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12791992; DOI=10.1126/science.1083523;
RA Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S.,
RA Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D.,
RA Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M.,
RA Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F.,
RA Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M.,
RA Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R.,
RA Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F.,
RA Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D.,
RA Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R.,
RA Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S.,
RA Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S.,
RA Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R.,
RA Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M.,
RA Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E.,
RA Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT "In-depth view of structure, activity, and evolution of rice chromosome
RT 10.";
RL Science 300:1566-1569(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=21807436; DOI=10.1016/j.jplph.2011.05.026;
RA Tang Y., Li M., Chen Y., Wu P., Wu G., Jiang H.;
RT "Knockdown of OsPAO and OsRCCR1 cause different plant death phenotypes in
RT rice.";
RL J. Plant Physiol. 168:1952-1959(2011).
CC -!- FUNCTION: Catalyzes the key reaction of chlorophyll catabolism,
CC porphyrin macrocycle cleavage of pheophorbide a (pheide a) to a primary
CC fluorescent catabolite (pFCC). Works in a two-step reaction with
CC pheophorbide a oxygenase (PaO) by reducing the C20/C1 double bond of
CC the intermediate, RCC. Belongs to the chlorophyll catabolic enzymes
CC (CCEs) (By similarity). May play a role in senescence and response to
CC wounding (PubMed:21807436). {ECO:0000250|UniProtKB:Q8LDU4,
CC ECO:0000269|PubMed:21807436}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 oxidized [2Fe-2S]-[ferredoxin] + primary fluorescent
CC chlorophyll catabolite = 3 H(+) + red chlorophyll catabolite + 2
CC reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:24752, Rhea:RHEA-
CC COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:58716,
CC ChEBI:CHEBI:77670; EC=1.3.7.12;
CC Evidence={ECO:0000250|UniProtKB:Q8LDU4};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:24754;
CC Evidence={ECO:0000305};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC degradation. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves (PubMed:21807436). Expressed at
CC low levels in roots, stems, panicles and seeds (PubMed:21807436).
CC {ECO:0000269|PubMed:21807436}.
CC -!- INDUCTION: Induced by wounding and senescence in leaves.
CC {ECO:0000269|PubMed:21807436}.
CC -!- MISCELLANEOUS: Plants silencing RCCR1 exhibit lesion-mimic spots on
CC leaves. {ECO:0000269|PubMed:21807436}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK13108.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAK52126.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF26414.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; JN604886; AEW31179.1; -; mRNA.
DR EMBL; AC078839; AAK13108.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC079936; AAK52126.1; ALT_SEQ; Genomic_DNA.
DR EMBL; DP000086; ABB47486.1; -; Genomic_DNA.
DR EMBL; AP008216; BAF26414.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014966; BAT10653.1; -; Genomic_DNA.
DR RefSeq; XP_015614206.1; XM_015758720.1.
DR AlphaFoldDB; Q338P6; -.
DR SMR; Q338P6; -.
DR STRING; 4530.OS10T0389200-01; -.
DR PaxDb; Q338P6; -.
DR PRIDE; Q338P6; -.
DR EnsemblPlants; Os10t0389200-01; Os10t0389200-01; Os10g0389200.
DR GeneID; 4348519; -.
DR Gramene; Os10t0389200-01; Os10t0389200-01; Os10g0389200.
DR KEGG; osa:4348519; -.
DR eggNOG; ENOG502QSTY; Eukaryota.
DR HOGENOM; CLU_073200_0_0_1; -.
DR OMA; MHLTVWL; -.
DR OrthoDB; 1382571at2759; -.
DR UniPathway; UPA00674; -.
DR Proteomes; UP000000763; Chromosome 10.
DR Proteomes; UP000059680; Chromosome 10.
DR GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR GO; GO:0051743; F:red chlorophyll catabolite reductase activity; IBA:GO_Central.
DR GO; GO:0015996; P:chlorophyll catabolic process; IBA:GO_Central.
DR InterPro; IPR009439; RCC_reductase.
DR PANTHER; PTHR34685; PTHR34685; 1.
DR Pfam; PF06405; RCC_reductase; 1.
PE 2: Evidence at transcript level;
KW Chlorophyll catabolism; Chloroplast; Oxidoreductase; Plastid;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..50
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 51..329
FT /note="Red chlorophyll catabolite reductase 1,
FT chloroplastic"
FT /id="PRO_0000449544"
FT REGION 1..61
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..20
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 163
FT /ligand="red chlorophyll catabolite"
FT /ligand_id="ChEBI:CHEBI:58716"
FT /evidence="ECO:0000250|UniProtKB:Q8LDU4"
FT BINDING 216..218
FT /ligand="red chlorophyll catabolite"
FT /ligand_id="ChEBI:CHEBI:58716"
FT /evidence="ECO:0000250|UniProtKB:Q8LDU4"
FT BINDING 299
FT /ligand="red chlorophyll catabolite"
FT /ligand_id="ChEBI:CHEBI:58716"
FT /evidence="ECO:0000250|UniProtKB:Q8LDU4"
SQ SEQUENCE 329 AA; 35551 MW; 7CCF2633F7B1EFBF CRC64;
MLQLRSPPPA TSSPSSAVSF PTLAPRLLPL RRRRRGAGSQ LGGKTSSAVR ASSAAAPGAT
EPEVMVEVAH REVARALASL AEARLGARLL PSAVPPDVAE FRSGGGAGNA VGSLDVRRGA
PGSTIDFMLQ SSLHCKVPNG AIDITSLLIF LNASTDAPHF LMEFIQGSPT SIVVLLDLLP
RKDLALHPEY IERYYENTQV DKQREKVEEL PQARPYRSRS LFVRSTFSLT AILMSIDCGQ
GGEGTLEEIV RGQLATAARA LLQIWLDSCA DHTSEMEEGE RENMIKRDQI VRSKSIEVDL
TSNLPRMFGP DVADRVIAEI QKAFGVQEA
