ID   RCCR_HORVU              Reviewed;         205 AA.
AC   Q9MTQ6;
DT   16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Red chlorophyll catabolite reductase;
DE            Short=RCC reductase;
DE            EC=1.3.7.12 {ECO:0000269|PubMed:12223836};
DE   AltName: Full=HvRCCR;
DE   Flags: Fragment;
GN   Name=rccR;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Express; TISSUE=Leaf;
RX   PubMed=10743659; DOI=10.1046/j.1365-313x.2000.00667.x;
RA   Wuethrich K.L., Bovet L., Hunziker P.E., Donnison I.S., Hoertensteiner S.;
RT   "Molecular cloning, functional expression and characterization of RCC
RT   reductase, involved in chlorophyll catabolism.";
RL   Plant J. 21:189-198(2000).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, CHARACTERIZATION, AND BIOPHYSICOCHEMICAL
RP   PROPERTIES.
RX   PubMed=12223836; DOI=10.1104/pp.115.2.677;
RA   Rodoni S., Vicentini F., Schellenberg M., Matile P., Hortensteiner S.;
RT   "Partial purification and characterization of red chlorophyll catabolite
RT   reductase, a stroma protein involved in chlorophyll breakdown.";
RL   Plant Physiol. 115:677-682(1997).
CC   -!- FUNCTION: Catalyzes the key reaction of chlorophyll catabolism,
CC       porphyrin macrocycle cleavage of pheophorbide a (pheide a) to a primary
CC       fluorescent catabolite (pFCC). Works in a two-step reaction with
CC       pheophorbide a oxygenase (PaO) by reducing the C20/C1 double bond of
CC       the intermediate, RCC. {ECO:0000269|PubMed:12223836}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 oxidized [2Fe-2S]-[ferredoxin] + primary fluorescent
CC         chlorophyll catabolite = 3 H(+) + red chlorophyll catabolite + 2
CC         reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:24752, Rhea:RHEA-
CC         COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:58716,
CC         ChEBI:CHEBI:77670; EC=1.3.7.12;
CC         Evidence={ECO:0000269|PubMed:12223836};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.6 mM for red chlorophyll catabolite
CC         {ECO:0000269|PubMed:12223836};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       degradation.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma.
CC   -!- TISSUE SPECIFICITY: In etiolated and green primary leaves. Low amount
CC       in roots.
CC   -!- DEVELOPMENTAL STAGE: Decreases during leaf senescence, but still
CC       present after 8 days of dark-induced senescence.
CC   -!- PTM: The N-terminus is blocked.
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DR   EMBL; AJ243066; CAB77705.1; -; mRNA.
DR   AlphaFoldDB; Q9MTQ6; -.
DR   SMR; Q9MTQ6; -.
DR   KEGG; ag:CAB77705; -.
DR   BRENDA; 1.3.7.12; 2687.
DR   SABIO-RK; Q9MTQ6; -.
DR   UniPathway; UPA00674; -.
DR   GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR   GO; GO:0051743; F:red chlorophyll catabolite reductase activity; IEA:InterPro.
DR   GO; GO:0015996; P:chlorophyll catabolic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR009439; RCC_reductase.
DR   PANTHER; PTHR34685; PTHR34685; 1.
DR   Pfam; PF06405; RCC_reductase; 1.
PE   1: Evidence at protein level;
KW   Chlorophyll catabolism; Chloroplast; NADP; Oxidoreductase; Plastid.
FT   CHAIN           <1..205
FT                   /note="Red chlorophyll catabolite reductase"
FT                   /id="PRO_0000097198"
FT   BINDING         39
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000305"
FT   BINDING         175
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000305"
FT   SITE            103
FT                   /note="Important for substrate stereospecificity"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   205 AA;  22868 MW;  835DC48C813D5AD7 CRC64;
     IDFMLQSSLH CKVPNGAIDI TSLFINLNAS TDAPHFIMEF IQGSPTSMVV LLDLLPRKDL
     ALHPEYIEKY YEDTEVDKQR KIIEQLPQAR PYLSPSLFVR SAFSPTAVFF TIDCGKGGEG
     TLEEIVHGHL ASVVKGILQI WLDTCASDAS EMEEGEREIM VKRDRTVRSK SIEVDLTANL
     PRMFGPDVSG RIIAEIRKAF GVQEG