ID   RCDA_ECOLI              Reviewed;         178 AA.
AC   P75811;
DT   27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   27-MAR-2002, sequence version 3.
DT   03-AUG-2022, entry version 150.
DE   RecName: Full=HTH-type transcriptional regulator RcdA;
DE   AltName: Full=Regulator of csgD;
GN   Name=rcdA; Synonyms=ybjK; OrderedLocusNames=b0846, JW5114;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   FUNCTION, DNA-BINDING, INDUCTION, AND GENE NAME.
RX   PubMed=23233451; DOI=10.1002/mbo3.42;
RA   Shimada T., Katayama Y., Kawakita S., Ogasawara H., Nakano M., Yamamoto K.,
RA   Ishihama A.;
RT   "A novel regulator RcdA of the csgD gene encoding the master regulator of
RT   biofilm formation in Escherichia coli.";
RL   MicrobiologyOpen 1:381-394(2012).
CC   -!- FUNCTION: Regulates the expression of a number of genes involved in
CC       biofilm formation and stress response. Target genes include six stress-
CC       response transcriptional regulators: csgD, which is a master regulator
CC       of biofilm formation, appY, sxy, ycgF, fimB and rcdA itself. This
CC       indicates that a large number of genes must be regulated indirectly via
CC       these transcriptional regulators. Acts by binding to the upstream
CC       region of its target genes. {ECO:0000269|PubMed:23233451}.
CC   -!- INDUCTION: Autoregulated. {ECO:0000269|PubMed:23233451}.
CC   -!- MISCELLANEOUS: Has a strong cooperative DNA-binding mode, leading to
CC       aggregation of RcdA-DNA complexes. {ECO:0000305|PubMed:23233451}.
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DR   EMBL; U00096; AAC73933.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35550.2; -; Genomic_DNA.
DR   PIR; F64822; F64822.
DR   RefSeq; NP_415367.1; NC_000913.3.
DR   RefSeq; WP_001295289.1; NZ_SSZK01000002.1.
DR   PDB; 5X5I; X-ray; 2.55 A; A/B/C/D/E/F/G/H=1-178.
DR   PDB; 7ACL; X-ray; 2.05 A; A/B=1-178.
DR   PDB; 7ACM; X-ray; 1.76 A; A/B=1-178.
DR   PDB; 7ACO; X-ray; 1.80 A; A/B=1-178.
DR   PDBsum; 5X5I; -.
DR   PDBsum; 7ACL; -.
DR   PDBsum; 7ACM; -.
DR   PDBsum; 7ACO; -.
DR   AlphaFoldDB; P75811; -.
DR   SMR; P75811; -.
DR   BioGRID; 4262832; 5.
DR   STRING; 511145.b0846; -.
DR   PaxDb; P75811; -.
DR   PRIDE; P75811; -.
DR   EnsemblBacteria; AAC73933; AAC73933; b0846.
DR   EnsemblBacteria; BAA35550; BAA35550; BAA35550.
DR   GeneID; 945473; -.
DR   KEGG; ecj:JW5114; -.
DR   KEGG; eco:b0846; -.
DR   PATRIC; fig|511145.12.peg.874; -.
DR   EchoBASE; EB3444; -.
DR   eggNOG; COG3226; Bacteria.
DR   HOGENOM; CLU_069356_21_2_6; -.
DR   InParanoid; P75811; -.
DR   OMA; SMTYHFD; -.
DR   PhylomeDB; P75811; -.
DR   BioCyc; EcoCyc:G6444-MON; -.
DR   PRO; PR:P75811; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:EcoliWiki.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:EcoliWiki.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEP:EcoCyc.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR   Pfam; PF00440; TetR_N; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF48498; SSF48498; 1.
DR   PROSITE; PS50977; HTH_TETR_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..178
FT                   /note="HTH-type transcriptional regulator RcdA"
FT                   /id="PRO_0000070634"
FT   DOMAIN          7..67
FT                   /note="HTH tetR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        30..49
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   HELIX           7..24
FT                   /evidence="ECO:0007829|PDB:7ACM"
FT   HELIX           26..28
FT                   /evidence="ECO:0007829|PDB:7ACM"
FT   HELIX           31..38
FT                   /evidence="ECO:0007829|PDB:7ACM"
FT   HELIX           42..48
FT                   /evidence="ECO:0007829|PDB:7ACM"
FT   HELIX           52..73
FT                   /evidence="ECO:0007829|PDB:7ACM"
FT   TURN            74..77
FT                   /evidence="ECO:0007829|PDB:7ACM"
FT   HELIX           81..93
FT                   /evidence="ECO:0007829|PDB:7ACM"
FT   HELIX           100..112
FT                   /evidence="ECO:0007829|PDB:7ACM"
FT   TURN            113..115
FT                   /evidence="ECO:0007829|PDB:7ACM"
FT   HELIX           117..134
FT                   /evidence="ECO:0007829|PDB:7ACM"
FT   TURN            135..137
FT                   /evidence="ECO:0007829|PDB:7ACM"
FT   HELIX           140..159
FT                   /evidence="ECO:0007829|PDB:7ACM"
FT   HELIX           166..177
FT                   /evidence="ECO:0007829|PDB:7ACM"
SQ   SEQUENCE   178 AA;  20307 MW;  B65B725AD11F45CB CRC64;
     MRRANDPQRR EKIIQATLEA VKLYGIHAVT HRKIATLAGV PLGSMTYYFS GIDELLLEAF
     SSFTEIMSRQ YQAFFSDVSD APGACQAITD MIYSSQVATP DNMELMYQLY ALASRKPLLK
     TVMQNWMQRS QQTLEQWFEP GTARALDAFI EGMTLHFVTD RKPLSREEIL RMVERVAG