RCEH_BLAVI
ID RCEH_BLAVI Reviewed; 258 AA.
AC P06008;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Reaction center protein H chain;
DE AltName: Full=Photosynthetic reaction center H subunit;
GN Name=puhA;
OS Blastochloris viridis (Rhodopseudomonas viridis).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Blastochloridaceae; Blastochloris.
OX NCBI_TaxID=1079;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FORMYLATION AT MET-1.
RC STRAIN=ATCC 19567 / DSM 133 / F;
RX PubMed=16453623; DOI=10.1002/j.1460-2075.1985.tb03835.x;
RA Michel H., Weyer K.A., Gruenberg H., Lottspeich F.;
RT "The `heavy' subunit of the photosynthetic reaction centre from
RT Rhodopseudomonas viridis: isolation of the gene, nucleotide and amino acid
RT sequence.";
RL EMBO J. 4:1667-1672(1985).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (3 ANGSTROMS).
RA Deisenhofer J., Epp O., Miki K., Huber R., Michel H.;
RT "Structure of the protein subunits in the photosynthetic reaction centre of
RT Rhodopseudomonas viridis at 3-A resolution.";
RL Nature 318:618-624(1985).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (3 ANGSTROMS).
RX PubMed=6392571; DOI=10.1016/s0022-2836(84)80011-x;
RA Deisenhofer J., Epp O., Miki K., Huber R., Michel H.;
RT "X-ray structure analysis of a membrane protein complex. Electron density
RT map at 3-A resolution and a model of the chromophores of the photosynthetic
RT reaction center from Rhodopseudomonas viridis.";
RL J. Mol. Biol. 180:385-398(1984).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS).
RC STRAIN=ATCC 19567 / DSM 133 / F;
RX PubMed=9351808; DOI=10.1016/s0969-2126(97)00285-2;
RA Lancaster C.R.D., Michel H.;
RT "The coupling of light-induced electron transfer and proton uptake as
RT derived from crystal structures of reaction centres from Rhodopseudomonas
RT viridis modified at the binding site of the secondary quinone, QB.";
RL Structure 5:1339-1359(1997).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).
RC STRAIN=ATCC 19567 / DSM 133 / F;
RX PubMed=10024457; DOI=10.1006/jmbi.1998.2532;
RA Lancaster C.R.D., Michel H.;
RT "Refined crystal structures of reaction centres from Rhodopseudomonas
RT viridis in complexes with the herbicide atrazine and two chiral atrazine
RT derivatives also lead to a new model of the bound carotenoid.";
RL J. Mol. Biol. 286:883-898(1999).
RN [6]
RP TOPOLOGY.
RX PubMed=2676514; DOI=10.1002/j.1460-2075.1989.tb08338.x;
RA Deisenhofer J., Michel H.;
RT "Nobel lecture. The photosynthetic reaction centre from the purple
RT bacterium Rhodopseudomonas viridis.";
RL EMBO J. 8:2149-2170(1989).
CC -!- FUNCTION: The reaction center is a membrane-bound complex that mediates
CC the initial photochemical event in the electron transfer process of
CC photosynthesis.
CC -!- COFACTOR:
CC Name=a bacteriochlorophyll; Xref=ChEBI:CHEBI:38201;
CC Note=Binds 4 bacteriochlorophylls per trimer.;
CC -!- COFACTOR:
CC Name=a bacteriopheophytin; Xref=ChEBI:CHEBI:60411;
CC Note=Binds 2 bacteriopheophytins per trimer.;
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Note=Binds 1 Fe cation per trimer.;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Note=Binds 4 Mg(2+) ions per trimer.;
CC -!- COFACTOR:
CC Name=a menaquinone; Xref=ChEBI:CHEBI:16374;
CC Note=Binds 1 menaquinone per trimer.;
CC -!- COFACTOR:
CC Name=a ubiquinone; Xref=ChEBI:CHEBI:16389;
CC Note=Binds 1 ubiquinone per trimer.;
CC -!- SUBUNIT: Heterotrimer composed of subunits L, M, and H.
CC -!- SUBCELLULAR LOCATION: Cellular chromatophore membrane; Single-pass
CC membrane protein.
CC -!- SIMILARITY: Belongs to the reaction center PuhA family. {ECO:0000305}.
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DR EMBL; X02659; CAA26495.1; -; Genomic_DNA.
DR PIR; A22841; A22841.
DR RefSeq; WP_055036342.1; NZ_LN907867.1.
DR PDB; 1DXR; X-ray; 2.00 A; H=1-258.
DR PDB; 1PRC; X-ray; 2.30 A; H=1-258.
DR PDB; 1R2C; X-ray; 2.86 A; H=1-258.
DR PDB; 1VRN; X-ray; 2.20 A; H=1-258.
DR PDB; 2I5N; X-ray; 1.96 A; H=1-258.
DR PDB; 2JBL; X-ray; 2.40 A; H=1-258.
DR PDB; 2PRC; X-ray; 2.45 A; H=1-258.
DR PDB; 2WJM; X-ray; 1.95 A; H=1-258.
DR PDB; 2WJN; X-ray; 1.86 A; H=1-258.
DR PDB; 2X5U; X-ray; 3.00 A; H=1-258.
DR PDB; 2X5V; X-ray; 3.00 A; H=1-258.
DR PDB; 3D38; X-ray; 3.21 A; H=1-258.
DR PDB; 3G7F; X-ray; 2.50 A; H=1-258.
DR PDB; 3PRC; X-ray; 2.40 A; H=1-258.
DR PDB; 3T6D; X-ray; 1.95 A; H=1-258.
DR PDB; 3T6E; X-ray; 1.92 A; H=1-258.
DR PDB; 4AC5; X-ray; 8.20 A; H=1-258.
DR PDB; 4CAS; X-ray; 3.50 A; D=1-258.
DR PDB; 5M7J; X-ray; 3.50 A; D=2-258.
DR PDB; 5M7K; X-ray; 3.50 A; D=1-258.
DR PDB; 5M7L; X-ray; 3.60 A; D=1-258.
DR PDB; 5NJ4; X-ray; 2.40 A; H=1-258.
DR PDB; 5O4C; X-ray; 2.80 A; H=1-258.
DR PDB; 5O64; X-ray; 3.30 A; H=2-258.
DR PDB; 5PRC; X-ray; 2.35 A; H=1-258.
DR PDB; 6ET5; EM; 3.00 A; H=1-258.
DR PDB; 6PRC; X-ray; 2.30 A; H=1-258.
DR PDB; 6ZHW; X-ray; 2.80 A; H=1-258.
DR PDB; 6ZI4; X-ray; 2.80 A; H=1-258.
DR PDB; 6ZI5; X-ray; 2.80 A; H=1-258.
DR PDB; 6ZI6; X-ray; 2.80 A; H=1-258.
DR PDB; 6ZI9; X-ray; 2.80 A; H=1-258.
DR PDB; 6ZIA; X-ray; 2.80 A; H=1-258.
DR PDB; 6ZID; X-ray; 2.80 A; H=1-258.
DR PDB; 7PRC; X-ray; 2.65 A; H=1-258.
DR PDBsum; 1DXR; -.
DR PDBsum; 1PRC; -.
DR PDBsum; 1R2C; -.
DR PDBsum; 1VRN; -.
DR PDBsum; 2I5N; -.
DR PDBsum; 2JBL; -.
DR PDBsum; 2PRC; -.
DR PDBsum; 2WJM; -.
DR PDBsum; 2WJN; -.
DR PDBsum; 2X5U; -.
DR PDBsum; 2X5V; -.
DR PDBsum; 3D38; -.
DR PDBsum; 3G7F; -.
DR PDBsum; 3PRC; -.
DR PDBsum; 3T6D; -.
DR PDBsum; 3T6E; -.
DR PDBsum; 4AC5; -.
DR PDBsum; 4CAS; -.
DR PDBsum; 5M7J; -.
DR PDBsum; 5M7K; -.
DR PDBsum; 5M7L; -.
DR PDBsum; 5NJ4; -.
DR PDBsum; 5O4C; -.
DR PDBsum; 5O64; -.
DR PDBsum; 5PRC; -.
DR PDBsum; 6ET5; -.
DR PDBsum; 6PRC; -.
DR PDBsum; 6ZHW; -.
DR PDBsum; 6ZI4; -.
DR PDBsum; 6ZI5; -.
DR PDBsum; 6ZI6; -.
DR PDBsum; 6ZI9; -.
DR PDBsum; 6ZIA; -.
DR PDBsum; 6ZID; -.
DR PDBsum; 7PRC; -.
DR AlphaFoldDB; P06008; -.
DR SMR; P06008; -.
DR IntAct; P06008; 1.
DR STRING; 1079.BVIR_576; -.
DR DrugBank; DB07552; (2R)-2-{[4-chloro-6-(ethylamino)-1,3,5-triazin-2-yl]amino}-2-methylbutanenitrile.
DR DrugBank; DB07551; (2S)-2-{[4-chloro-6-(ethylamino)-1,3,5-triazin-2-yl]amino}-2-methylbutanenitrile.
DR DrugBank; DB07392; Atrazine.
DR DrugBank; DB04147; Dodecyldimethylamine N-oxide.
DR DrugBank; DB04464; N-Formylmethionine.
DR DrugBank; DB08215; Terbutryn.
DR DrugBank; DB08689; Ubiquinone Q1.
DR DrugBank; DB08690; Ubiquinone Q2.
DR OMA; YAFWIFF; -.
DR OrthoDB; 1044122at2; -.
DR EvolutionaryTrace; P06008; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030077; C:plasma membrane light-harvesting complex; IEA:InterPro.
DR GO; GO:0042717; C:plasma membrane-derived chromatophore membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042314; F:bacteriochlorophyll binding; IEA:UniProtKB-KW.
DR GO; GO:0045156; F:electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity; IEA:InterPro.
DR GO; GO:0019684; P:photosynthesis, light reaction; IEA:InterPro.
DR CDD; cd00226; PRCH; 1.
DR Gene3D; 3.90.50.10; -; 1.
DR Gene3D; 4.10.540.10; -; 1.
DR InterPro; IPR014747; Bac_photo_RC_H_C.
DR InterPro; IPR005652; Photo_RC_H.
DR InterPro; IPR015810; Photo_RC_H_N.
DR InterPro; IPR037097; Photo_RC_H_N_sf.
DR InterPro; IPR027275; PRC-brl_dom.
DR InterPro; IPR011033; PRC_barrel-like_sf.
DR Pfam; PF05239; PRC; 1.
DR Pfam; PF03967; PRCH; 1.
DR SUPFAM; SSF50346; SSF50346; 1.
DR TIGRFAMs; TIGR01150; puhA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bacteriochlorophyll; Chlorophyll; Chromophore;
KW Electron transport; Formylation; Membrane; Photosynthesis; Reaction center;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..258
FT /note="Reaction center protein H chain"
FT /id="PRO_0000090396"
FT TOPO_DOM 1..11
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:2676514"
FT TRANSMEM 12..30
FT /note="Helical"
FT TOPO_DOM 31..258
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:2676514"
FT MOD_RES 1
FT /note="N-formylmethionine"
FT /evidence="ECO:0000269|PubMed:16453623"
FT STRAND 2..5
FT /evidence="ECO:0007829|PDB:2X5U"
FT HELIX 7..9
FT /evidence="ECO:0007829|PDB:3T6D"
FT HELIX 12..30
FT /evidence="ECO:0007829|PDB:2WJN"
FT HELIX 32..35
FT /evidence="ECO:0007829|PDB:2WJN"
FT STRAND 38..40
FT /evidence="ECO:0007829|PDB:2WJN"
FT STRAND 46..48
FT /evidence="ECO:0007829|PDB:3T6D"
FT STRAND 51..53
FT /evidence="ECO:0007829|PDB:3T6E"
FT HELIX 56..60
FT /evidence="ECO:0007829|PDB:3T6E"
FT STRAND 66..69
FT /evidence="ECO:0007829|PDB:2WJN"
FT STRAND 75..79
FT /evidence="ECO:0007829|PDB:2WJN"
FT STRAND 90..96
FT /evidence="ECO:0007829|PDB:2WJN"
FT STRAND 101..105
FT /evidence="ECO:0007829|PDB:2WJN"
FT HELIX 107..110
FT /evidence="ECO:0007829|PDB:2WJN"
FT HELIX 113..115
FT /evidence="ECO:0007829|PDB:2WJN"
FT STRAND 122..124
FT /evidence="ECO:0007829|PDB:2WJN"
FT STRAND 130..136
FT /evidence="ECO:0007829|PDB:2WJN"
FT TURN 137..139
FT /evidence="ECO:0007829|PDB:2WJN"
FT STRAND 156..158
FT /evidence="ECO:0007829|PDB:2WJN"
FT STRAND 160..162
FT /evidence="ECO:0007829|PDB:2X5V"
FT STRAND 164..174
FT /evidence="ECO:0007829|PDB:2WJN"
FT TURN 175..178
FT /evidence="ECO:0007829|PDB:2WJN"
FT STRAND 179..187
FT /evidence="ECO:0007829|PDB:2WJN"
FT TURN 188..191
FT /evidence="ECO:0007829|PDB:2WJN"
FT STRAND 192..197
FT /evidence="ECO:0007829|PDB:2WJN"
FT HELIX 198..200
FT /evidence="ECO:0007829|PDB:2WJN"
FT STRAND 208..210
FT /evidence="ECO:0007829|PDB:1R2C"
FT HELIX 215..220
FT /evidence="ECO:0007829|PDB:2WJN"
FT STRAND 225..228
FT /evidence="ECO:0007829|PDB:2WJN"
FT HELIX 232..248
FT /evidence="ECO:0007829|PDB:2WJN"
FT HELIX 251..253
FT /evidence="ECO:0007829|PDB:2WJN"
SQ SEQUENCE 258 AA; 28499 MW; 280D7A94093AFA87 CRC64;
MYHGALAQHL DIAQLVWYAQ WLVIWTVVLL YLRREDRREG YPLVEPLGLV KLAPEDGQVY
ELPYPKTFVL PHGGTVTVPR RRPETRELKL AQTDGFEGAP LQPTGNPLVD AVGPASYAER
AEVVDATVDG KAKIVPLRVA TDFSIAEGDV DPRGLPVVAA DGVEAGTVTD LWVDRSEHYF
RYLELSVAGS ARTALIPLGF CDVKKDKIVV TSILSEQFAN VPRLQSRDQI TLREEDKVSA
YYAGGLLYAT PERAESLL
