RCEH_CERS4
ID RCEH_CERS4 Reviewed; 260 AA.
AC Q3J170; P11846; Q9RFD9;
DT 24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Reaction center protein H chain;
DE AltName: Full=Photosynthetic reaction center H subunit;
GN Name=puhA; OrderedLocusNames=RHOS4_18960; ORFNames=RSP_0291;
OS Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG
OS 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) (Rhodobacter
OS sphaeroides).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Cereibacter.
OX NCBI_TaxID=272943;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10648776; DOI=10.1093/nar/28.4.862;
RA Choudhary M., Kaplan S.;
RT "DNA sequence analysis of the photosynthesis region of Rhodobacter
RT sphaeroides 2.4.1.";
RL Nucleic Acids Res. 28:862-867(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203
RC / NCIMB 8253 / ATH 2.4.1.;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C.,
RA Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.;
RT "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1.";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The reaction center is a membrane-bound complex that mediates
CC the initial photochemical event in the electron transfer process of
CC photosynthesis. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=a bacteriochlorophyll; Xref=ChEBI:CHEBI:38201;
CC Evidence={ECO:0000250};
CC Note=Binds 4 bacteriochlorophylls per trimer. {ECO:0000250};
CC -!- COFACTOR:
CC Name=a bacteriopheophytin; Xref=ChEBI:CHEBI:60411;
CC Evidence={ECO:0000250};
CC Note=Binds 2 bacteriopheophytins per trimer. {ECO:0000250};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC Note=Binds 1 Fe cation per trimer. {ECO:0000250};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 4 Mg(2+) ions per trimer. {ECO:0000250};
CC -!- COFACTOR:
CC Name=a ubiquinone; Xref=ChEBI:CHEBI:16389; Evidence={ECO:0000250};
CC Note=Binds 2 ubiquinone per trimer. {ECO:0000250};
CC -!- SUBUNIT: Heterotrimer composed of subunits L, M, and H. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cellular chromatophore membrane; Single-pass
CC membrane protein.
CC -!- SIMILARITY: Belongs to the reaction center PuhA family. {ECO:0000305}.
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DR EMBL; AF195122; AAF24269.1; -; Genomic_DNA.
DR EMBL; CP000143; ABA79464.1; -; Genomic_DNA.
DR PIR; T50725; T50725.
DR RefSeq; WP_002720455.1; NZ_CP030271.1.
DR RefSeq; YP_353365.1; NC_007493.2.
DR PDB; 2WX5; X-ray; 2.63 A; H=1-260.
DR PDB; 4IN5; X-ray; 2.20 A; H=1-260.
DR PDB; 4IN6; X-ray; 2.70 A; H=1-260.
DR PDB; 4N7L; X-ray; 2.85 A; H=11-251.
DR PDB; 5LRI; X-ray; 2.40 A; H=1-260.
DR PDB; 7PIL; EM; 2.50 A; H=1-246.
DR PDBsum; 2WX5; -.
DR PDBsum; 4IN5; -.
DR PDBsum; 4IN6; -.
DR PDBsum; 4N7L; -.
DR PDBsum; 5LRI; -.
DR PDBsum; 7PIL; -.
DR AlphaFoldDB; Q3J170; -.
DR SMR; Q3J170; -.
DR STRING; 272943.RSP_0291; -.
DR DrugBank; DB04147; Dodecyldimethylamine N-oxide.
DR EnsemblBacteria; ABA79464; ABA79464; RSP_0291.
DR GeneID; 57470609; -.
DR GeneID; 67447024; -.
DR KEGG; rsp:RSP_0291; -.
DR PATRIC; fig|272943.9.peg.2235; -.
DR eggNOG; COG3861; Bacteria.
DR OMA; YAFWIFF; -.
DR PhylomeDB; Q3J170; -.
DR Proteomes; UP000002703; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030077; C:plasma membrane light-harvesting complex; IEA:InterPro.
DR GO; GO:0042717; C:plasma membrane-derived chromatophore membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042314; F:bacteriochlorophyll binding; IEA:UniProtKB-KW.
DR GO; GO:0045156; F:electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity; IEA:InterPro.
DR GO; GO:0019684; P:photosynthesis, light reaction; IEA:InterPro.
DR CDD; cd00226; PRCH; 1.
DR Gene3D; 3.90.50.10; -; 1.
DR Gene3D; 4.10.540.10; -; 1.
DR InterPro; IPR014747; Bac_photo_RC_H_C.
DR InterPro; IPR005652; Photo_RC_H.
DR InterPro; IPR015810; Photo_RC_H_N.
DR InterPro; IPR037097; Photo_RC_H_N_sf.
DR InterPro; IPR027275; PRC-brl_dom.
DR InterPro; IPR011033; PRC_barrel-like_sf.
DR Pfam; PF05239; PRC; 1.
DR Pfam; PF03967; PRCH; 1.
DR SUPFAM; SSF50346; SSF50346; 1.
DR TIGRFAMs; TIGR01150; puhA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bacteriochlorophyll; Chlorophyll; Chromophore;
KW Electron transport; Membrane; Photosynthesis; Reaction center;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..260
FT /note="Reaction center protein H chain"
FT /id="PRO_0000090395"
FT TOPO_DOM 1..11
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 12..31
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 32..260
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT CONFLICT 38
FT /note="E -> Q (in Ref. 1; AAF24269)"
FT /evidence="ECO:0000305"
FT CONFLICT 81
FT /note="E -> K (in Ref. 1; AAF24269)"
FT /evidence="ECO:0000305"
FT CONFLICT 107
FT /note="D -> N (in Ref. 1; AAF24269)"
FT /evidence="ECO:0000305"
FT HELIX 12..34
FT /evidence="ECO:0007829|PDB:4IN5"
FT STRAND 37..39
FT /evidence="ECO:0007829|PDB:4IN5"
FT STRAND 47..49
FT /evidence="ECO:0007829|PDB:4IN5"
FT STRAND 62..66
FT /evidence="ECO:0007829|PDB:4IN5"
FT TURN 67..70
FT /evidence="ECO:0007829|PDB:4IN5"
FT STRAND 71..76
FT /evidence="ECO:0007829|PDB:4IN5"
FT STRAND 87..93
FT /evidence="ECO:0007829|PDB:4IN5"
FT STRAND 98..102
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 104..107
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 110..112
FT /evidence="ECO:0007829|PDB:4IN5"
FT STRAND 127..133
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 134..136
FT /evidence="ECO:0007829|PDB:4IN5"
FT STRAND 141..145
FT /evidence="ECO:0007829|PDB:4IN5"
FT STRAND 152..155
FT /evidence="ECO:0007829|PDB:4IN5"
FT STRAND 160..170
FT /evidence="ECO:0007829|PDB:4IN5"
FT TURN 171..174
FT /evidence="ECO:0007829|PDB:4IN5"
FT STRAND 175..182
FT /evidence="ECO:0007829|PDB:4IN5"
FT STRAND 188..192
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 193..195
FT /evidence="ECO:0007829|PDB:4IN5"
FT STRAND 197..199
FT /evidence="ECO:0007829|PDB:2WX5"
FT STRAND 203..205
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 210..212
FT /evidence="ECO:0007829|PDB:4IN5"
FT TURN 213..215
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 227..243
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 245..247
FT /evidence="ECO:0007829|PDB:4IN5"
SQ SEQUENCE 260 AA; 28035 MW; 7E0B913B63D4DEB0 CRC64;
MVGVTAFGNF DLASLAIYSF WIFLAGLIYY LQTENMREGY PLENEDGTPA ANQGPFPLPK
PKTFILPHGR GTLTVPGPES EDRPIALART AVSEGFPHAP TGDPMKDGVG PASWVARRDL
PELDGHGHNK IKPMKAAAGF HVSAGKNPIG LPVRGCDLEI AGKVVDIWVD IPEQMARFLE
VELKDGSTRL LPMQMVKVQS NRVHVNALSS DLFAGIPTIK SPTEVTLLEE DKICGYVAGG
LMYAAPKRKS VVAAMLAEYA
