RCEH_CERSP
ID RCEH_CERSP Reviewed; 260 AA.
AC P0C0Y7; P11846; Q9RFD9;
DT 24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Reaction center protein H chain;
DE AltName: Full=Photosynthetic reaction center H subunit;
GN Name=puhA;
OS Cereibacter sphaeroides (Rhodobacter sphaeroides).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Cereibacter.
OX NCBI_TaxID=1063;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3329732; DOI=10.1002/prot.340010405;
RA Williams J.C., Steiner L.A., Feher G.;
RT "Primary structure of the reaction center from Rhodopseudomonas
RT sphaeroides.";
RL Proteins 1:312-325(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Y;
RA Picaud M.;
RL Submitted (OCT-1991) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
RX PubMed=2036404; DOI=10.1021/bi00236a005;
RA Chang C.-H., El-Kabbani O., Tiede D., Norris J., Schiffer M.;
RT "Structure of the membrane-bound protein photosynthetic reaction center
RT from Rhodobacter sphaeroides.";
RL Biochemistry 30:5352-5360(1991).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
RC STRAIN=R-26;
RX PubMed=3054889; DOI=10.1073/pnas.85.22.8487;
RA Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C.;
RT "Structure of the reaction center from Rhodobacter sphaeroides R-26:
RT protein-cofactor (quinones and Fe2+) interactions.";
RL Proc. Natl. Acad. Sci. U.S.A. 85:8487-8491(1988).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
RC STRAIN=R-26;
RX PubMed=2819866; DOI=10.1073/pnas.84.17.6162;
RA Allen J.P., Feher G., Yeates T.O., Komiya H., Rees D.C.;
RT "Structure of the reaction center from Rhodobacter sphaeroides R-26: the
RT protein subunits.";
RL Proc. Natl. Acad. Sci. U.S.A. 84:6162-6166(1987).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS).
RX PubMed=9537989; DOI=10.1021/bi971717a;
RA McAuley-Hecht K.E., Fyfe P.K., Ridge J.P., Prince S.M., Hunter C.N.,
RA Isaacs N.W., Cogdell R.J., Jones M.R.;
RT "Structural studies of wild-type and mutant reaction centers from an
RT antenna-deficient strain of Rhodobacter sphaeroides: monitoring the optical
RT properties of the complex from bacterial cell to crystal.";
RL Biochemistry 37:4740-4750(1998).
CC -!- FUNCTION: The reaction center is a membrane-bound complex that mediates
CC the initial photochemical event in the electron transfer process of
CC photosynthesis.
CC -!- COFACTOR:
CC Name=a bacteriochlorophyll; Xref=ChEBI:CHEBI:38201;
CC Note=Binds 4 bacteriochlorophylls per trimer.;
CC -!- COFACTOR:
CC Name=a bacteriopheophytin; Xref=ChEBI:CHEBI:60411;
CC Note=Binds 2 bacteriopheophytins per trimer.;
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Note=Binds 1 Fe cation per trimer.;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Note=Binds 4 Mg(2+) ions per trimer.;
CC -!- COFACTOR:
CC Name=a ubiquinone; Xref=ChEBI:CHEBI:16389;
CC Note=Binds 2 ubiquinone per trimer.;
CC -!- SUBUNIT: Heterotrimer composed of subunits L, M, and H.
CC -!- SUBCELLULAR LOCATION: Cellular chromatophore membrane; Single-pass
CC membrane protein.
CC -!- SIMILARITY: Belongs to the reaction center PuhA family. {ECO:0000305}.
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DR EMBL; M18412; AAA26176.1; -; Genomic_DNA.
DR EMBL; X63378; CAA44976.1; -; Genomic_DNA.
DR PIR; A26538; A26538.
DR RefSeq; WP_002720455.1; NZ_WTFI01000017.1.
DR PDB; 1AIG; X-ray; 2.60 A; H/P=1-260.
DR PDB; 1AIJ; X-ray; 2.20 A; H/T=1-260.
DR PDB; 1DS8; X-ray; 2.50 A; H/T=1-260.
DR PDB; 1DV3; X-ray; 2.50 A; H/T=1-260.
DR PDB; 1DV6; X-ray; 2.50 A; H/T=1-260.
DR PDB; 1E14; X-ray; 2.70 A; H=1-260.
DR PDB; 1E6D; X-ray; 2.30 A; H=1-260.
DR PDB; 1F6N; X-ray; 2.80 A; H=1-260.
DR PDB; 1FNP; X-ray; 2.60 A; H=1-260.
DR PDB; 1FNQ; X-ray; 2.60 A; H=1-260.
DR PDB; 1JGW; X-ray; 2.80 A; H=1-260.
DR PDB; 1JGX; X-ray; 3.01 A; H=1-260.
DR PDB; 1JGY; X-ray; 2.70 A; H=1-260.
DR PDB; 1JGZ; X-ray; 2.70 A; H=1-260.
DR PDB; 1JH0; X-ray; 3.50 A; H=1-260.
DR PDB; 1K6L; X-ray; 3.10 A; H=1-260.
DR PDB; 1K6N; X-ray; 3.10 A; H=1-260.
DR PDB; 1KBY; X-ray; 2.50 A; H=1-260.
DR PDB; 1L9B; X-ray; 2.40 A; H=1-260.
DR PDB; 1L9J; X-ray; 3.25 A; H/T=1-260.
DR PDB; 1M3X; X-ray; 2.55 A; H=1-260.
DR PDB; 1MPS; X-ray; 2.55 A; H=1-260.
DR PDB; 1OGV; X-ray; 2.35 A; H=11-260.
DR PDB; 1PCR; X-ray; 2.65 A; H=1-260.
DR PDB; 1PSS; X-ray; 3.00 A; H=12-248.
DR PDB; 1PST; X-ray; 3.00 A; H=12-248.
DR PDB; 1QOV; X-ray; 2.10 A; H=1-260.
DR PDB; 1RG5; X-ray; 2.50 A; H=1-260.
DR PDB; 1RGN; X-ray; 2.80 A; H=1-260.
DR PDB; 1RQK; X-ray; 2.70 A; H=1-260.
DR PDB; 1RVJ; X-ray; 2.75 A; H=1-260.
DR PDB; 1RY5; X-ray; 2.10 A; H=1-260.
DR PDB; 1RZH; X-ray; 1.80 A; H=1-260.
DR PDB; 1RZZ; X-ray; 2.40 A; H/T=1-260.
DR PDB; 1S00; X-ray; 2.60 A; H/T=1-260.
DR PDB; 1UMX; X-ray; 2.80 A; H=1-260.
DR PDB; 1YF6; X-ray; 2.25 A; H=1-260.
DR PDB; 1YST; X-ray; 3.00 A; H=1-260.
DR PDB; 1Z9J; X-ray; 4.50 A; C=1-260.
DR PDB; 1Z9K; X-ray; 4.60 A; C=1-260.
DR PDB; 2BNP; X-ray; 2.70 A; C=1-260.
DR PDB; 2BNS; X-ray; 2.50 A; C=1-260.
DR PDB; 2BOZ; X-ray; 2.40 A; H=1-260.
DR PDB; 2GMR; X-ray; 2.50 A; H=1-260.
DR PDB; 2GNU; X-ray; 2.20 A; H=11-245.
DR PDB; 2HG3; X-ray; 2.70 A; H=1-260.
DR PDB; 2HG9; X-ray; 2.45 A; H=1-260.
DR PDB; 2HH1; X-ray; 2.55 A; H=1-260.
DR PDB; 2HHK; X-ray; 2.50 A; H=1-260.
DR PDB; 2HIT; X-ray; 2.75 A; H=1-260.
DR PDB; 2HJ6; X-ray; 3.00 A; H=1-260.
DR PDB; 2J8C; X-ray; 1.87 A; H=1-260.
DR PDB; 2J8D; X-ray; 2.07 A; H=1-260.
DR PDB; 2JIY; X-ray; 2.20 A; H=1-260.
DR PDB; 2JJ0; X-ray; 2.80 A; H=1-260.
DR PDB; 2RCR; X-ray; 3.10 A; H=1-260.
DR PDB; 2UWS; X-ray; 2.90 A; H=1-260.
DR PDB; 2UWT; X-ray; 2.50 A; H=1-260.
DR PDB; 2UWU; X-ray; 2.04 A; H=1-260.
DR PDB; 2UWV; X-ray; 2.13 A; H=1-260.
DR PDB; 2UWW; X-ray; 2.05 A; H=1-260.
DR PDB; 2UX3; X-ray; 2.50 A; H=1-260.
DR PDB; 2UX4; X-ray; 2.51 A; H=1-260.
DR PDB; 2UX5; X-ray; 2.21 A; H=1-260.
DR PDB; 2UXJ; X-ray; 2.25 A; H=1-260.
DR PDB; 2UXK; X-ray; 2.31 A; H=1-260.
DR PDB; 2UXL; X-ray; 2.88 A; H=1-260.
DR PDB; 2UXM; X-ray; 2.70 A; H=1-260.
DR PDB; 3DSY; X-ray; 3.00 A; H=1-260.
DR PDB; 3DTA; X-ray; 3.20 A; H=1-260.
DR PDB; 3DTR; X-ray; 3.10 A; H=1-260.
DR PDB; 3DTS; X-ray; 3.10 A; H=1-260.
DR PDB; 3DU2; X-ray; 3.10 A; H=1-260.
DR PDB; 3DU3; X-ray; 2.80 A; H=1-260.
DR PDB; 3DUQ; X-ray; 2.70 A; H=1-260.
DR PDB; 3I4D; X-ray; 2.01 A; H=1-260.
DR PDB; 3V3Y; X-ray; 2.80 A; H=10-250.
DR PDB; 3V3Z; X-ray; 2.90 A; H=10-250.
DR PDB; 3ZUM; X-ray; 2.50 A; H=1-260.
DR PDB; 3ZUW; X-ray; 2.31 A; H=1-260.
DR PDB; 4H99; X-ray; 2.97 A; H=11-250.
DR PDB; 4H9L; X-ray; 2.77 A; H=11-250.
DR PDB; 4HBH; X-ray; 2.93 A; H=11-250.
DR PDB; 4HBJ; X-ray; 2.74 A; H=11-250.
DR PDB; 4IN7; X-ray; 2.85 A; H=1-250.
DR PDB; 4LWY; X-ray; 2.90 A; H=1-260.
DR PDB; 4N7K; X-ray; 2.85 A; H=11-251.
DR PDB; 4RCR; X-ray; 2.80 A; H=1-260.
DR PDB; 4TQQ; X-ray; 2.50 A; H=11-250.
DR PDB; 4V9G; X-ray; 7.78 A; AH/BH=1-260.
DR PDB; 5LSE; X-ray; 2.50 A; H=1-260.
DR PDB; 5V33; X-ray; 3.49 A; H=11-250.
DR PDB; 6Z02; X-ray; 2.10 A; H=10-250.
DR PDB; 6Z1J; X-ray; 2.10 A; H=10-250.
DR PDB; 6Z27; X-ray; 2.10 A; H=1-250.
DR PDB; 7MH3; X-ray; 2.30 A; H=1-259.
DR PDB; 7MH4; X-ray; 2.48 A; H=1-259.
DR PDB; 7MH5; X-ray; 2.85 A; H=1-259.
DR PDB; 7MH8; X-ray; 2.75 A; H=1-259.
DR PDB; 7MH9; X-ray; 3.10 A; H=1-259.
DR PDBsum; 1AIG; -.
DR PDBsum; 1AIJ; -.
DR PDBsum; 1DS8; -.
DR PDBsum; 1DV3; -.
DR PDBsum; 1DV6; -.
DR PDBsum; 1E14; -.
DR PDBsum; 1E6D; -.
DR PDBsum; 1F6N; -.
DR PDBsum; 1FNP; -.
DR PDBsum; 1FNQ; -.
DR PDBsum; 1JGW; -.
DR PDBsum; 1JGX; -.
DR PDBsum; 1JGY; -.
DR PDBsum; 1JGZ; -.
DR PDBsum; 1JH0; -.
DR PDBsum; 1K6L; -.
DR PDBsum; 1K6N; -.
DR PDBsum; 1KBY; -.
DR PDBsum; 1L9B; -.
DR PDBsum; 1L9J; -.
DR PDBsum; 1M3X; -.
DR PDBsum; 1MPS; -.
DR PDBsum; 1OGV; -.
DR PDBsum; 1PCR; -.
DR PDBsum; 1PSS; -.
DR PDBsum; 1PST; -.
DR PDBsum; 1QOV; -.
DR PDBsum; 1RG5; -.
DR PDBsum; 1RGN; -.
DR PDBsum; 1RQK; -.
DR PDBsum; 1RVJ; -.
DR PDBsum; 1RY5; -.
DR PDBsum; 1RZH; -.
DR PDBsum; 1RZZ; -.
DR PDBsum; 1S00; -.
DR PDBsum; 1UMX; -.
DR PDBsum; 1YF6; -.
DR PDBsum; 1YST; -.
DR PDBsum; 1Z9J; -.
DR PDBsum; 1Z9K; -.
DR PDBsum; 2BNP; -.
DR PDBsum; 2BNS; -.
DR PDBsum; 2BOZ; -.
DR PDBsum; 2GMR; -.
DR PDBsum; 2GNU; -.
DR PDBsum; 2HG3; -.
DR PDBsum; 2HG9; -.
DR PDBsum; 2HH1; -.
DR PDBsum; 2HHK; -.
DR PDBsum; 2HIT; -.
DR PDBsum; 2HJ6; -.
DR PDBsum; 2J8C; -.
DR PDBsum; 2J8D; -.
DR PDBsum; 2JIY; -.
DR PDBsum; 2JJ0; -.
DR PDBsum; 2RCR; -.
DR PDBsum; 2UWS; -.
DR PDBsum; 2UWT; -.
DR PDBsum; 2UWU; -.
DR PDBsum; 2UWV; -.
DR PDBsum; 2UWW; -.
DR PDBsum; 2UX3; -.
DR PDBsum; 2UX4; -.
DR PDBsum; 2UX5; -.
DR PDBsum; 2UXJ; -.
DR PDBsum; 2UXK; -.
DR PDBsum; 2UXL; -.
DR PDBsum; 2UXM; -.
DR PDBsum; 3DSY; -.
DR PDBsum; 3DTA; -.
DR PDBsum; 3DTR; -.
DR PDBsum; 3DTS; -.
DR PDBsum; 3DU2; -.
DR PDBsum; 3DU3; -.
DR PDBsum; 3DUQ; -.
DR PDBsum; 3I4D; -.
DR PDBsum; 3V3Y; -.
DR PDBsum; 3V3Z; -.
DR PDBsum; 3ZUM; -.
DR PDBsum; 3ZUW; -.
DR PDBsum; 4H99; -.
DR PDBsum; 4H9L; -.
DR PDBsum; 4HBH; -.
DR PDBsum; 4HBJ; -.
DR PDBsum; 4IN7; -.
DR PDBsum; 4LWY; -.
DR PDBsum; 4N7K; -.
DR PDBsum; 4RCR; -.
DR PDBsum; 4TQQ; -.
DR PDBsum; 4V9G; -.
DR PDBsum; 5LSE; -.
DR PDBsum; 5V33; -.
DR PDBsum; 6Z02; -.
DR PDBsum; 6Z1J; -.
DR PDBsum; 6Z27; -.
DR PDBsum; 7MH3; -.
DR PDBsum; 7MH4; -.
DR PDBsum; 7MH5; -.
DR PDBsum; 7MH8; -.
DR PDBsum; 7MH9; -.
DR AlphaFoldDB; P0C0Y7; -.
DR SMR; P0C0Y7; -.
DR DIP; DIP-60491N; -.
DR IntAct; P0C0Y7; 2.
DR DrugBank; DB04147; Dodecyldimethylamine N-oxide.
DR DrugBank; DB08215; Terbutryn.
DR DrugBank; DB08690; Ubiquinone Q2.
DR TCDB; 3.E.2.1.1; the photosynthetic reaction center (prc) family.
DR GeneID; 57470609; -.
DR GeneID; 67447024; -.
DR OMA; YAFWIFF; -.
DR OrthoDB; 1044122at2; -.
DR EvolutionaryTrace; P0C0Y7; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030077; C:plasma membrane light-harvesting complex; IEA:InterPro.
DR GO; GO:0042717; C:plasma membrane-derived chromatophore membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042314; F:bacteriochlorophyll binding; IEA:UniProtKB-KW.
DR GO; GO:0045156; F:electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity; IEA:InterPro.
DR GO; GO:0019684; P:photosynthesis, light reaction; IEA:InterPro.
DR CDD; cd00226; PRCH; 1.
DR Gene3D; 3.90.50.10; -; 1.
DR Gene3D; 4.10.540.10; -; 1.
DR InterPro; IPR014747; Bac_photo_RC_H_C.
DR InterPro; IPR005652; Photo_RC_H.
DR InterPro; IPR015810; Photo_RC_H_N.
DR InterPro; IPR037097; Photo_RC_H_N_sf.
DR InterPro; IPR027275; PRC-brl_dom.
DR InterPro; IPR011033; PRC_barrel-like_sf.
DR Pfam; PF05239; PRC; 1.
DR Pfam; PF03967; PRCH; 1.
DR SUPFAM; SSF50346; SSF50346; 1.
DR TIGRFAMs; TIGR01150; puhA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bacteriochlorophyll; Chlorophyll; Chromophore;
KW Electron transport; Membrane; Photosynthesis; Reaction center;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..260
FT /note="Reaction center protein H chain"
FT /id="PRO_0000090394"
FT TOPO_DOM 1..11
FT /note="Periplasmic"
FT TRANSMEM 12..31
FT /note="Helical"
FT TOPO_DOM 32..260
FT /note="Cytoplasmic"
FT TURN 6..8
FT /evidence="ECO:0007829|PDB:2J8D"
FT HELIX 12..34
FT /evidence="ECO:0007829|PDB:1RZH"
FT STRAND 37..39
FT /evidence="ECO:0007829|PDB:1RZH"
FT STRAND 40..43
FT /evidence="ECO:0007829|PDB:4RCR"
FT STRAND 47..49
FT /evidence="ECO:0007829|PDB:1RZH"
FT STRAND 50..52
FT /evidence="ECO:0007829|PDB:1YST"
FT STRAND 56..58
FT /evidence="ECO:0007829|PDB:1PSS"
FT STRAND 62..66
FT /evidence="ECO:0007829|PDB:1RZH"
FT HELIX 67..69
FT /evidence="ECO:0007829|PDB:1RZH"
FT STRAND 71..76
FT /evidence="ECO:0007829|PDB:1RZH"
FT STRAND 87..93
FT /evidence="ECO:0007829|PDB:1RZH"
FT STRAND 98..102
FT /evidence="ECO:0007829|PDB:1RZH"
FT HELIX 104..107
FT /evidence="ECO:0007829|PDB:1RZH"
FT HELIX 110..112
FT /evidence="ECO:0007829|PDB:1RZH"
FT STRAND 127..133
FT /evidence="ECO:0007829|PDB:1RZH"
FT HELIX 134..136
FT /evidence="ECO:0007829|PDB:1RZH"
FT STRAND 137..139
FT /evidence="ECO:0007829|PDB:1L9B"
FT STRAND 141..145
FT /evidence="ECO:0007829|PDB:1RZH"
FT HELIX 148..150
FT /evidence="ECO:0007829|PDB:1YST"
FT STRAND 152..155
FT /evidence="ECO:0007829|PDB:1RZH"
FT STRAND 156..158
FT /evidence="ECO:0007829|PDB:1FNP"
FT STRAND 160..170
FT /evidence="ECO:0007829|PDB:1RZH"
FT TURN 171..174
FT /evidence="ECO:0007829|PDB:1RZH"
FT STRAND 175..182
FT /evidence="ECO:0007829|PDB:1RZH"
FT STRAND 184..186
FT /evidence="ECO:0007829|PDB:4RCR"
FT STRAND 188..192
FT /evidence="ECO:0007829|PDB:1RZH"
FT HELIX 193..195
FT /evidence="ECO:0007829|PDB:1RZH"
FT STRAND 197..199
FT /evidence="ECO:0007829|PDB:2HG9"
FT STRAND 203..207
FT /evidence="ECO:0007829|PDB:1RZH"
FT HELIX 210..212
FT /evidence="ECO:0007829|PDB:1RZH"
FT TURN 213..215
FT /evidence="ECO:0007829|PDB:2UWU"
FT STRAND 220..223
FT /evidence="ECO:0007829|PDB:6Z1J"
FT HELIX 227..243
FT /evidence="ECO:0007829|PDB:1RZH"
FT HELIX 245..247
FT /evidence="ECO:0007829|PDB:1RZH"
FT STRAND 248..250
FT /evidence="ECO:0007829|PDB:2RCR"
FT HELIX 251..254
FT /evidence="ECO:0007829|PDB:2J8D"
SQ SEQUENCE 260 AA; 28035 MW; 7E0B913B63D4DEB0 CRC64;
MVGVTAFGNF DLASLAIYSF WIFLAGLIYY LQTENMREGY PLENEDGTPA ANQGPFPLPK
PKTFILPHGR GTLTVPGPES EDRPIALART AVSEGFPHAP TGDPMKDGVG PASWVARRDL
PELDGHGHNK IKPMKAAAGF HVSAGKNPIG LPVRGCDLEI AGKVVDIWVD IPEQMARFLE
VELKDGSTRL LPMQMVKVQS NRVHVNALSS DLFAGIPTIK SPTEVTLLEE DKICGYVAGG
LMYAAPKRKS VVAAMLAEYA
